P59497 · RNT_BUCBP

Function

function

Trims short 3' overhangs of a variety of RNA species, leaving a one or two nucleotide 3' overhang. Responsible for the end-turnover of tRNA: specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds two Mg2+ per subunit. The active form of the enzyme binds two Mg2+ ions in its active site. The first Mg2+ forms only one salt bridge with the protein.

Features

Showing features for binding site, site, active site.

121720406080100120140160180200
TypeIDPosition(s)Description
Binding site23Mg2+ 1 (UniProtKB | ChEBI); catalytic
Binding site23Mg2+ 2 (UniProtKB | ChEBI); catalytic
Binding site25Mg2+ 2 (UniProtKB | ChEBI); catalytic
Site29Important for substrate binding and specificity
Site77Important for substrate binding and specificity
Site124Important for substrate binding and specificity
Site146Important for substrate binding and specificity
Active site181Proton donor/acceptor
Binding site181Mg2+ 2 (UniProtKB | ChEBI); catalytic
Binding site186Mg2+ 2 (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function3'-5' exonuclease activity
Molecular Functionmagnesium ion binding
Molecular Functionnucleic acid binding
Molecular FunctionRNA exonuclease activity, producing 5'-phosphomonoesters
Biological ProcessDNA replication proofreading
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease T
  • EC number
  • Alternative names
    • Exoribonuclease T
      (RNase T
      )

Gene names

    • Name
      rnt
    • Ordered locus names
      bbp_177

Organism names

Accessions

  • Primary accession
    P59497

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002089581-217Ribonuclease T

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain20-194Exonuclease

Sequence similarities

Belongs to the RNase T family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    217
  • Mass (Da)
    24,637
  • Last updated
    2003-04-04 v1
  • Checksum
    9B8E084D0643E87D
MCYSKINNSLRKRFRTFYPVVIDIETAGFNPETDAILEIAIITLKMNEFGLLEKEHLLHFHIQPFKGSRIDKKAIEFHGIDPFSPLRRAISEYEALYSIFNLIHKGIKSNNCTKSIIVAHNAIFDYNFLTAAITRTKIKNNPFHSFVIFDTATLSGLAVGQTVLARACKAIGLTFDNNQAHSALYDTQQTANLFCKIVNRWKTLGGWPPNDTRTIKL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE016826
EMBL· GenBank· DDBJ
AAO26909.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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