P58816 · MCRZ_METTM
- ProteinMethyl-coenzyme M reductase II subunit gamma
- GenemrtG
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids265 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.
Catalytic activity
- coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methaneThis reaction proceeds in the forward direction.
Cofactor
Note: Binds 2 coenzyme F430 non-covalently per MCR complex. Coenzyme F430 is a yellow nickel porphinoid.
Pathway
One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | coenzyme-B sulfoethylthiotransferase activity | |
Biological Process | methanogenesis |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMethyl-coenzyme M reductase II subunit gamma
- EC number
- Short namesMCR II gamma
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Methanomada group > Methanobacteria > Methanobacteriales > Methanobacteriaceae > Methanothermobacter
Accessions
- Primary accessionP58816
- Secondary accessions
Proteomes
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000147481 | 2-265 | Methyl-coenzyme M reductase II subunit gamma | |||
Sequence: SYKAQYTPGETQIAENRRKHMDPDYEFRKLREVSDEDLVKVLGHRNPGESYKSVHPPLDEMDFEEDIVRDMVEPIQGAKEGVRVRYIQFADSMYNAPAQPYDRARTYMWRYRGVDTGTLSGRQVIEMRELDLEGVSKELVETELFDPATTGIRGATVHGHSLRLDENGLMFDALQRYVFDEEKGHVVYVKDQVGRPLDEPVDMGQPLGEDELKKITTIYRKDNIAMRDDKEAIEVVENIHTGRTLGGFGMDVFKDDLRKRLGDD |
Proteomic databases
Expression
Developmental stage
There are two MCR complexes in this bacteria. MCR II is expressed in the early growth phase. Late growth cells contain mostly MCR I.
Interaction
Subunit
MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length265
- Mass (Da)30,535
- Last updated2010-11-30 v3
- ChecksumEA917838AE5A3CA9
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001710 EMBL· GenBank· DDBJ | ADL59092.1 EMBL· GenBank· DDBJ | Genomic DNA |