P58733 · IPYR_PSEAO
- ProteinInorganic pyrophosphatase
- Geneppa
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids180 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Hydrolyzes PPi generated in anabolic reactions.
Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.
Catalytic activity
- diphosphate + H2O = H+ + 2 phosphate
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
2.9 μM | PPi |
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 28 | substrate | ||||
Sequence: K | ||||||
Binding site | 42 | substrate | ||||
Sequence: R | ||||||
Binding site | 54 | substrate | ||||
Sequence: Y | ||||||
Binding site | 66 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 71 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 71 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 102 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 139 | substrate | ||||
Sequence: Y |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | inorganic diphosphate phosphatase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | phosphate-containing compound metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameInorganic pyrophosphatase
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageBacteria > Cyanobacteriota > Cyanophyceae > Pseudanabaenales > Pseudanabaenaceae > Pseudanabaena
Accessions
- Primary accessionP58733
- Secondary accessions
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000137519 | 1-180 | Inorganic pyrophosphatase | |||
Sequence: MDLSRIPPQPKAGILNVLIEIPAGSKNKYEFDKDLNAFALDRVLYSSVQYPYDYGFVPITNNLADDGDPLDGMVIMVPPTFPGVATARPIGMLQMVDGGDRDEKFLCVPAKDPRYTYVKSANDLAGHRLDEIFEFFRSYKNLFKKPTEFFGWKGDVAGLPLVEECVKNYYKTYCKNDHGK |
Interaction
Subunit
Homohexamer.
Structure
Sequence
- Sequence statusComplete
- Length180
- Mass (Da)20,332
- Last updated2002-02-11 v1
- ChecksumF2A84DC1A9038A42
Keywords
- Technical term