P58131 · RPOC1_EUGLO
- ProteinDNA-directed RNA polymerase subunit beta'
- GenerpoC1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids575 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Mg2+ ion per subunit.
Note: Binds 1 Zn2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 64 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 66 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 85 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 88 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 440 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 442 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 444 | Mg2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | DNA-directed RNA polymerase complex | |
Cellular Component | plastid | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | metal ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta'
- EC number
- Alternative names
Gene names
Encoded on
- Non-photosynthetic plastid
Organism names
- Strain
- Taxonomic lineageEukaryota > Discoba > Euglenozoa > Euglenida > Spirocuta > Euglenophyceae > Euglenales > Euglenaceae > Euglena
Accessions
- Primary accessionP58131
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000067862 | 1-575 | DNA-directed RNA polymerase subunit beta' | ||
Interaction
Subunit
In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Family & Domains
Sequence similarities
Belongs to the RNA polymerase beta' chain family. RpoC1 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length575
- Mass (Da)67,075
- Last updated2001-06-01 v1
- MD5 Checksum9D7EFB7282ECA388CF71D477C26F6D3B
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ294725 EMBL· GenBank· DDBJ | CAC24618.1 EMBL· GenBank· DDBJ | Genomic DNA |