P57849 · THII_PASMU

Function

function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

Catalytic activity

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site183-184ATP (UniProtKB | ChEBI)
Binding site265ATP (UniProtKB | ChEBI)
Binding site287ATP (UniProtKB | ChEBI)
Binding site296ATP (UniProtKB | ChEBI)
Active site454Cysteine persulfide intermediate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uracil-4 sulfurtransferase activity
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process
Biological Processthiazole biosynthetic process
Biological ProcesstRNA 4-thiouridine biosynthesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA sulfurtransferase
  • EC number
  • Alternative names
    • Sulfur carrier protein ThiS sulfurtransferase
    • Thiamine biosynthesis protein ThiI
    • tRNA 4-thiouridine synthase

Gene names

    • Name
      thiI
    • Ordered locus names
      PM0535

Organism names

Accessions

  • Primary accession
    P57849

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00001548541-480tRNA sulfurtransferase
Disulfide bond344↔454Redox-active

Keywords

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain61-165THUMP
Domain404-479Rhodanese

Sequence similarities

Belongs to the ThiI family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    480
  • Mass (Da)
    54,428
  • Last updated
    2001-04-27 v1
  • Checksum
    C1A1761F825128DD
MKFVIKLFPEIMIKSESVRKRFVKILTGNIRNILTKHDETIAVVRHWDYIEVRSKKEENRPHLIELLGRIPGIHHFLEVDEKPFTTIHDIFEQTLQDIGTSLENKTFCVRVRRKGKHTFNSLDVERYVGGGLNQHIPSAKVQLSKPDVTVRIDIENDNMMLIKARHVGIGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWQRYSASHKVRFVAINFEGVVGEILEKVDNGQMGVVLKRMMVRAASRIAERFGIQAIVTGEALGQVSSQTLTNLRLIDEASESLVLRPLITHDKEQIIAMAKEIGTDDIAKSMPEFCGVISKNPTVKAIKAKIEQEERHFDFAVLESAVQNAQYLDIRQIAEQTEKEVVAVDTVAVLSAQDVILDIRSPEETDEKPLNMENVQLMPFYKLSSQFANLDQSKNYLLYCERGVMSKLQALYLKEQGFSNVKVFRQ

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE004439
EMBL· GenBank· DDBJ
AAK02619.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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