P57695 · GLRA1_BOVIN
- ProteinGlycine receptor subunit alpha-1
- GeneGLRA1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids457 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Channel opening is also triggered by taurine and beta-alanine. Channel characteristics depend on the subunit composition; heteropentameric channels are activated by lower glycine levels and display faster desensitization (By similarity).
Plays an important role in the down-regulation of neuronal excitability (PubMed:11178872).
Contributes to the generation of inhibitory postsynaptic currents. Channel activity is potentiated by ethanol (By similarity).
Potentiation of channel activity by intoxicating levels of ethanol contribute to the sedative effects of ethanol (By similarity).
Plays an important role in the down-regulation of neuronal excitability (PubMed:11178872).
Contributes to the generation of inhibitory postsynaptic currents. Channel activity is potentiated by ethanol (By similarity).
Potentiation of channel activity by intoxicating levels of ethanol contribute to the sedative effects of ethanol (By similarity).
Miscellaneous
The alpha subunit binds strychnine.
Catalytic activity
- chloride(in) = chloride(out)
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 220 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 222 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 230-235 | strychnine (UniProtKB | ChEBI) | ||||
Sequence: YNTGKF | ||||||
Binding site | 243 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Site | 289 | Important for obstruction of the ion pore in the closed conformation | ||||
Sequence: L |
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlycine receptor subunit alpha-1
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos
Accessions
- Primary accessionP57695
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Postsynaptic cell membrane ; Multi-pass membrane protein
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 29-250 | Extracellular | ||||
Sequence: ARSASKPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVSCNIFINSFGSIAETTMDYRVNIFLRQQWNDPRLAYNEYPDDSLDLDPSMLDSIWKPDLFFANEKGAHFHEITTDNKLLRISRNGNVLYSIRITLTLACPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQEQGAVQVADGLTLPQFILKEEKDLRYCTKHYNTGKFTCIEARFHLERQMGY | ||||||
Transmembrane | 251-272 | Helical; Name=1 | ||||
Sequence: YLIQMYIPSLLIVILSWISFWI | ||||||
Topological domain | 273-277 | Cytoplasmic | ||||
Sequence: NMDAA | ||||||
Transmembrane | 278-298 | Helical; Name=2 | ||||
Sequence: PARVGLGITTVLTMTTQSSGS | ||||||
Topological domain | 299-309 | Extracellular | ||||
Sequence: RASLPKVSYVK | ||||||
Transmembrane | 310-330 | Helical; Name=3 | ||||
Sequence: AIDIWMAVCLLFVFSALLEYA | ||||||
Topological domain | 331-425 | Cytoplasmic | ||||
Sequence: AVNFVSRQHKELLRFRRKRRHHKSPMLNLFQEDEAGEGRFNFSAYGMGPACLQAKDGISVKGANNSNTTNPPPAPSKSPEEMRKLFIQRAKKIDK | ||||||
Transmembrane | 426-446 | Helical; Name=4 | ||||
Sequence: ISRIGFPMAFLIFNMFYWIIY | ||||||
Topological domain | 447-457 | Extracellular | ||||
Sequence: KIVRREDVHNQ |
Keywords
- Cellular component
Phenotypes & Variants
Involvement in disease
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 49 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for signal, chain, glycosylation, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-28 | |||||
Sequence: MYSFNTLRLYLWETIVFFSLAASKEAEA | ||||||
Chain | PRO_0000000411 | 29-457 | Glycine receptor subunit alpha-1 | |||
Sequence: ARSASKPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVSCNIFINSFGSIAETTMDYRVNIFLRQQWNDPRLAYNEYPDDSLDLDPSMLDSIWKPDLFFANEKGAHFHEITTDNKLLRISRNGNVLYSIRITLTLACPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQEQGAVQVADGLTLPQFILKEEKDLRYCTKHYNTGKFTCIEARFHLERQMGYYLIQMYIPSLLIVILSWISFWINMDAAPARVGLGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRRHHKSPMLNLFQEDEAGEGRFNFSAYGMGPACLQAKDGISVKGANNSNTTNPPPAPSKSPEEMRKLFIQRAKKIDKISRIGFPMAFLIFNMFYWIIYKIVRREDVHNQ | ||||||
Glycosylation | 66 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 166↔180 | |||||
Sequence: CPMDLKNFPMDVQTC | ||||||
Disulfide bond | 226↔237 | |||||
Sequence: CTKHYNTGKFTC |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Detected on spinal cord neurons (at protein level). Detected in spinal cord.
Gene expression databases
Interaction
Subunit
Homopentamer (in vitro). Interacts with GLRB to form heteropentameric channels; this is probably the predominant form in vivo. Heteropentamer composed of two GLRA1 and three GLRB. Heteropentamer composed of three GLRA1 and two GLRB. Both homopentamers and heteropentamers form functional ion channels, but their characteristics are subtly different. Interacts with GLRB (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 391-410 | Disordered | ||||
Sequence: KGANNSNTTNPPPAPSKSPE |
Domain
The channel pore is formed by pentameric assembly of the second transmembrane domain from all five subunits. Channel opening is effected by an outward rotation of the transmembrane domains that increases the diameter of the pore.
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA1 sub-subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 2 isoforms produced by Alternative splicing.
P57695-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Namea
- Length457
- Mass (Da)52,614
- Last updated2000-12-08 v1
- Checksum0C60A814C8ADFDDE
P57695-2
- Nameb
- Differences from canonical
- 354-361: Missing
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_021141 | 354-361 | in isoform b | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF268375 EMBL· GenBank· DDBJ | AAG14346.1 EMBL· GenBank· DDBJ | mRNA | ||
AF268366 EMBL· GenBank· DDBJ | AAG41140.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268358 EMBL· GenBank· DDBJ | AAG41140.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268360 EMBL· GenBank· DDBJ | AAG41140.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268361 EMBL· GenBank· DDBJ | AAG41140.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268359 EMBL· GenBank· DDBJ | AAG41140.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268362 EMBL· GenBank· DDBJ | AAG41140.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268364 EMBL· GenBank· DDBJ | AAG41140.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268365 EMBL· GenBank· DDBJ | AAG41140.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268363 EMBL· GenBank· DDBJ | AAG41140.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268366 EMBL· GenBank· DDBJ | AAG41141.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268358 EMBL· GenBank· DDBJ | AAG41141.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268360 EMBL· GenBank· DDBJ | AAG41141.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268361 EMBL· GenBank· DDBJ | AAG41141.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268359 EMBL· GenBank· DDBJ | AAG41141.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268362 EMBL· GenBank· DDBJ | AAG41141.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268364 EMBL· GenBank· DDBJ | AAG41141.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268365 EMBL· GenBank· DDBJ | AAG41141.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF268363 EMBL· GenBank· DDBJ | AAG41141.1 EMBL· GenBank· DDBJ | Genomic DNA |