P56913 · NUOF2_RHIME

Function

function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
FMN (UniProtKB | Rhea| CHEBI:58210 )

Note: Binds 1 FMN.
[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site53-62NAD+ (UniProtKB | ChEBI)
Binding site165-212FMN (UniProtKB | ChEBI)
Binding site342[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site345[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site348[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site388[4Fe-4S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentrespiratory chain complex I
Molecular Function4 iron, 4 sulfur cluster binding
Molecular FunctionFMN binding
Molecular Functionmetal ion binding
Molecular FunctionNAD binding
Molecular FunctionNADH dehydrogenase (ubiquinone) activity
Molecular Functionquinone binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADH-quinone oxidoreductase subunit F 2
  • EC number
  • Alternative names
    • NADH dehydrogenase I subunit F 2
    • NDH-1 subunit F 2

Gene names

    • Name
      nuoF2
    • ORF names
      SMa1525
    • Ordered locus names
      RA0829

Encoded on

  • Plasmid pSymA (megaplasmid 1)

Organism names

Accessions

  • Primary accession
    P56913

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001185771-421NADH-quinone oxidoreductase subunit F 2

Keywords

Structure

Family & Domains

Sequence similarities

Belongs to the complex I 51 kDa subunit family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    421
  • Mass (Da)
    45,504
  • Last updated
    2001-11-02 v2
  • MD5 Checksum
    565731CDD82F5389C5F8E628F874833F
MFEPVLLRNVDVPDGHLLSTYEAGGGYRALRKALGEYTPDEIVELVKESNLRGRGGAGFPTGMKWSFVPKAAGKPKYLCCNADEGEPGTFKDRIIMERDPHQLIEGLAVSAYAIGAETAYVYIRGEYVTAIRRMEQAIAEAHENGYLGIGILGSGFNFMVHIHRGAGAYICGEETAMLESLEGKRAQPRLKPPFPAVAGLYASPTVINNVETLACVPHIVMRGSAWFRGIGPDRSPGPKLYCLSGQVRKPGLYELPMGISLRELVEEHAGGPLPGRKVKAVIPGGVSAPVIPEGELEVGMDFDSLTAAGSMLGSAGVVVIDDSTCMVKLATRIIEFFHHESCGKCTPCREGLDWTVKVLRRIEAGEGETGDLEQLEMLCKGIFGNTFCALGDGAAMGLRAALKHFRAEFVAHIEERRCPFH

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict149in Ref. 1; CAB51634
Sequence conflict155in Ref. 1; CAB51634
Sequence conflict407in Ref. 1; CAB51634

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AJ245399
EMBL· GenBank· DDBJ
CAB51634.1
EMBL· GenBank· DDBJ
Genomic DNA
AE006469
EMBL· GenBank· DDBJ
AAK65487.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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