P56254 · KAB1_OLDAF
- ProteinKalata-B1
- GeneOAK1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids124 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Probably participates in a plant defense mechanism. Has antibiotic activity. Has a diuretic effect. Has a uterotonic effect in humans. Active against the Gram-positive S.aureus with a minimum inhibition concentration of approximately 0.2 microM. Relatively ineffective against Gram-negative bacteria such as E.coli and P.aeruginosa. Inhibitory effect on the growth and development of larvae from H.punctigera. The unmodified form has hemolytic activity, the oxidized form lacks hemolytic activity. If the protein is linearized, hemolytic activity is lost.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Biological Process | defense response to bacterium | |
Biological Process | killing of cells of another organism |
Keywords
- Molecular function
- Biological process
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameKalata-B1
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Gentianales > Rubiaceae > Rubioideae > Spermacoceae > Hedyotis-Oldenlandia complex > Oldenlandia
Accessions
- Primary accessionP56254
Phenotypes & Variants
Pharmaceutical
The uteroactive properties of Kalata have been discovered by African traditional medicine. It is used as an ingredient of a herbal tea to accelerate childbirth.
PTM/Processing
Features
Showing features for signal, propeptide, peptide, cross-link, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-22 | |||||
Sequence: MAKFTVCLLLCLLLAAFVGAFG | ||||||
Propeptide | PRO_0000006617 | 23-88 | ||||
Sequence: SELSDSHKTTLVNEIAEKMLQRKILDGVEATLVTDVAEKMFLRKMKAEAKTSETADQVFLKQLQLK | ||||||
Peptide | PRO_0000006618 | 89-117 | Kalata-B1 | |||
Sequence: GLPVCGETCVGGTCNTPGCTCSWPVCTRN | ||||||
Cross-link | 89↔117 | Cyclopeptide (Gly-Asn) | ||||
Sequence: GLPVCGETCVGGTCNTPGCTCSWPVCTRN | ||||||
Disulfide bond | 93↔107 | |||||
Sequence: CGETCVGGTCNTPGC | ||||||
Disulfide bond | 97↔109 | |||||
Sequence: CVGGTCNTPGCTC | ||||||
Disulfide bond | 102↔114 | |||||
Sequence: CNTPGCTCSWPVC | ||||||
Propeptide | PRO_0000006619 | 118-124 | ||||
Sequence: GLPSLAA |
Post-translational modification
Kalata-B1 is a cyclic peptide which occurs in three forms: with unmodified Trp-111, with Trp-111 oxidized to form oxindolylalanine and with Trp-111 oxidized to form N-formylkynurenine. Oxidation is enhanced by exposure to sunlight.
Keywords
- PTM
Expression
Tissue specificity
Leaves and stems. Lower in roots.
Structure
Family & Domains
Domain
The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.
Sequence similarities
Belongs to the cyclotide family. Moebius subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length124
- Mass (Da)13,271
- Last updated2001-12-05 v3
- Checksum4EAD1D69318FCCC9
Mass Spectrometry
Keywords
- Technical term