P56254 · KAB1_OLDAF

  • Protein
    Kalata-B1
  • Gene
    OAK1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Probably participates in a plant defense mechanism. Has antibiotic activity. Has a diuretic effect. Has a uterotonic effect in humans. Active against the Gram-positive S.aureus with a minimum inhibition concentration of approximately 0.2 microM. Relatively ineffective against Gram-negative bacteria such as E.coli and P.aeruginosa. Inhibitory effect on the growth and development of larvae from H.punctigera. The unmodified form has hemolytic activity, the oxidized form lacks hemolytic activity. If the protein is linearized, hemolytic activity is lost.

Caution

The oxidation forms of Trp-111 are subject of controversy and could be the artifactual results of sample handling.

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Biological Processdefense response to bacterium
Biological Processkilling of cells of another organism

Keywords

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Kalata-B1

Gene names

    • Name
      OAK1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Gentianales > Rubiaceae > Rubioideae > Spermacoceae > Hedyotis-Oldenlandia complex > Oldenlandia

Accessions

  • Primary accession
    P56254

Phenotypes & Variants

Pharmaceutical

The uteroactive properties of Kalata have been discovered by African traditional medicine. It is used as an ingredient of a herbal tea to accelerate childbirth.

PTM/Processing

Features

Showing features for signal, propeptide, peptide, cross-link, disulfide bond.

TypeIDPosition(s)Description
Signal1-22
PropeptidePRO_000000661723-88
PeptidePRO_000000661889-117Kalata-B1
Cross-link89↔117Cyclopeptide (Gly-Asn)
Disulfide bond93↔107
Disulfide bond97↔109
Disulfide bond102↔114
PropeptidePRO_0000006619118-124

Post-translational modification

Kalata-B1 is a cyclic peptide which occurs in three forms: with unmodified Trp-111, with Trp-111 oxidized to form oxindolylalanine and with Trp-111 oxidized to form N-formylkynurenine. Oxidation is enhanced by exposure to sunlight.

Keywords

Expression

Tissue specificity

Leaves and stems. Lower in roots.

Family & Domains

Domain

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similarities

Belongs to the cyclotide family. Moebius subfamily.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    124
  • Mass (Da)
    13,271
  • Last updated
    2001-12-05 v3
  • Checksum
    4EAD1D69318FCCC9
MAKFTVCLLLCLLLAAFVGAFGSELSDSHKTTLVNEIAEKMLQRKILDGVEATLVTDVAEKMFLRKMKAEAKTSETADQVFLKQLQLKGLPVCGETCVGGTCNTPGCTCSWPVCTRNGLPSLAA

Mass Spectrometry

Molecular mass is 2,892 Da. Determined by Electrospray.
Molecular mass is 2,982.4 Da. Determined by Electrospray.
Molecular mass is 2,908.4 Da. Determined by Electrospray. With oxindolylalanine.
Molecular mass is 2,924.4 Da. Determined by Electrospray. With N-formylkynurenine.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF393825
EMBL· GenBank· DDBJ
AAL05477.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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