Molecular cloning, sequencing, and heterologous expression of the vaoA gene from Penicillium simplicissimum CBS 170.90 encoding vanillyl-alcohol oxidase.Benen J.A.E., Sanchez-Torres P., Wagemaker M.J.M., Fraaije M.W., van Berkel W.J.H., Visser J.View abstractCited forNUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 6-30 AND 130-148StrainATCC 90172 / CBS 170.90 / PAZ 1CategoriesSequencesSourceUniProtKB reviewed (Swiss-Prot)PubMedEurope PMCJ. Biol. Chem. 273:7865-7872 (1998)Cited in1
Crystallization and preliminary X-ray analysis of the flavoenzyme vanillyl-alcohol oxidase from Penicillium simplicissimum.Mattevi A., Fraaije M.W., Coda A., van Berkel W.J.H.View abstractCited forX-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)StrainATCC 90172 / CBS 170.90 / PAZ 1CategoriesStructureSourceUniProtKB reviewed (Swiss-Prot)PubMedEurope PMCProteins 27:601-603 (1997)Cited in11
Covalent flavinylation is essential for efficient redox catalysis in vanillyl-alcohol oxidase.Fraaije M.W., van den Heuvel R.H.H., van Berkel W.J.H., Mattevi A.View abstractCited forX-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF MUTANTSStrainATCC 90172 / CBS 170.90 / PAZ 1CategoriesPhenotypes & Variants, StructureSourceUniProtKB reviewed (Swiss-Prot)PubMedEurope PMCJ. Biol. Chem. 274:35514-35520 (1999)Cited in1
Asp-170 is crucial for the redox properties of vanillyl-alcohol oxidase.van den Heuvel R.H.H., Fraaije M.W., Mattevi A., van Berkel W.J.H.View abstractCited forX-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF D170S MUTANTCategoriesPhenotypes & Variants, StructureSourceUniProtKB reviewed (Swiss-Prot)PubMedEurope PMCJ. Biol. Chem. 275:14799-14808 (2000)Cited in1
Inversion of stereospecificity of vanillyl-alcohol oxidase.van den Heuvel R.H.H., Fraaije M.W., Ferrer M., Mattevi A., van Berkel W.J.H.View abstractCited forX-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) OF D170S/T457E MUTANTCategoriesPhenotypes & Variants, StructureSourceUniProtKB reviewed (Swiss-Prot)PubMedEurope PMCProc. Natl. Acad. Sci. U.S.A. 97:9455-9460 (2000)Cited in1
Structural analysis of flavinylation in vanillyl-alcohol oxidase.Fraaije M.W., van den Heuvel R.H.H., van Berkel W.J.H., Mattevi A.View abstractCited forX-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF MUTANTSStrainATCC 90172 / CBS 170.90 / PAZ 1CategoriesPhenotypes & Variants, StructureSourceUniProtKB reviewed (Swiss-Prot)PubMedEurope PMCJ. Biol. Chem. 275:38654-38658 (2000)Cited in1
Subcellular localization of vanillyl-alcohol oxidase in Penicillium simplicissimum.Fraaije M.W., Sjollema K.A., Veenhuis M., van Berkel W.J.H.View abstractCited forSUBCELLULAR LOCATIONCategoriesSubcellular LocationSourceUniProtKB reviewed (Swiss-Prot)PubMedEurope PMCFEBS Lett. 422:65-68 (1998)Cited in1
Purification and characterization of vanillyl-alcohol oxidase from Penicillium simplicissimum. A novel aromatic alcohol oxidase containing covalently bound FAD.de Jong E., van Berkel W.J.H., van der Zwan R.P., de Bont J.A.M.View abstractCited forCHARACTERIZATIONStrainATCC 90172 / CBS 170.90 / PAZ 1SourceUniProtKB reviewed (Swiss-Prot)PubMedEurope PMCEur. J. Biochem. 208:651-657 (1992)Cited in1
Substrate specificity of flavin-dependent vanillyl-alcohol oxidase from Penicillium simplicissimum. Evidence for the production of 4- hydroxycinnamyl alcohols from 4-allylphenols.Fraaije M.W., Veeger C., van Berkel W.J.H.View abstractCited forSUBSTRATE SPECIFICITYStrainATCC 90172 / CBS 170.90 / PAZ 1CategoriesFunctionSourceUniProtKB reviewed (Swiss-Prot)PubMedEurope PMCEur. J. Biochem. 234:271-277 (1995)Cited in1
Crystal structures and inhibitor binding in the octameric flavoenzyme vanillyl-alcohol oxidase: the shape of the active-site cavity controls substrate specificity.Mattevi A., Fraaije M.W., Mozzarelli A., Olivi L., Coda A., van Berkel W.J.View abstractCategoriesStructureSourcePDB: 1AHU, PDB: 1AHV, PDB: 1AHZ, PDB: 1VAO, PDB: 2VAOPubMedEurope PMCStructure 5:907-920 (1997)Mapped to1
Two tyrosine residues, Tyr-108 and Tyr-503, are responsible for the deprotonation of phenolic substrates in vanillyl-alcohol oxidase.Ewing T.A., Nguyen Q.T., Allan R.C., Gygli G., Romero E., Binda C., Fraaije M.W., Mattevi A., van Berkel W.J.H.View abstractCategoriesStructureSourcePDB: 5MXJ, PDB: 5MXUPubMedEurope PMCJ Biol Chem 292:14668-14679 (2017)Mapped to1
Laboratory-evolved vanillyl-alcohol oxidase produces natural vanillin.van den Heuvel R.H., van den Berg W.A., Rovida S., van Berkel W.J.View abstractCategoriesStructureSourcePDB: 1W1J, PDB: 1W1K, PDB: 1W1L, PDB: 1W1MPubMedEurope PMCJ Biol Chem 279:33492-33500 (2004)Mapped to1