P55353 · FCL_SINFN
- ProteinGDP-L-fucose synthase
- Genefcl
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids314 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.
Catalytic activity
- GDP-beta-L-fucose + NADP+ = GDP-4-dehydro-alpha-D-rhamnose + H+ + NADPH
Pathway
Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 2/2.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 15-21 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: GHKGMVG | ||||||
Binding site | 109-112 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: LGSS | ||||||
Site | 111 | Important for catalytic activity | ||||
Sequence: S | ||||||
Site | 113 | Important for catalytic activity | ||||
Sequence: C | ||||||
Active site | 140 | Proton donor/acceptor | ||||
Sequence: Y | ||||||
Binding site | 144 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 167-170 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: PTNL | ||||||
Binding site | 183 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 191 | substrate | ||||
Sequence: K | ||||||
Binding site | 206 | substrate | ||||
Sequence: W | ||||||
Binding site | 213 | substrate | ||||
Sequence: R | ||||||
Binding site | 273 | substrate | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | GDP-L-fucose synthase activity | |
Molecular Function | isomerase activity | |
Molecular Function | NADP+ binding | |
Biological Process | 'de novo' GDP-L-fucose biosynthetic process | |
Biological Process | nodulation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGDP-L-fucose synthase
- EC number
- Alternative names
Gene names
Encoded on
- Plasmid sym pNGR234a
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Rhizobiaceae > Sinorhizobium/Ensifer group > Sinorhizobium
Accessions
- Primary accessionP55353
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000174360 | 1-314 | GDP-L-fucose synthase | |||
Sequence: MPMYLLDGKRIWVAGHKGMVGSAIIRSLASEDCEVIVADRQKLDLTRQEEVEKFLLKEKPHAVIMAAAKVGGILANDTMPADFIYQNLIMEANVIEGSFRSGVEKLLFLGSSCIYPKYAAQPIREEALLTGPLEPTNEWYAIAKIAGIKLCQAYRKQYGANFISAMPTNLYGPRDKFDLNSSHVVPALIRKAHEAKIKDLGCLSIWGSGTPTRDFLYSEDCSDALVFLLKHYSETEHINIGSGGEISIIELAHIVCRVVGFKGDIVFDTSKPDGTPRKLLSSERLVSMGWRPKTSLELGLAKSYESFVSNVADN |
Structure
Sequence
- Sequence statusComplete
- Length314
- Mass (Da)34,674
- Last updated1997-11-01 v1
- Checksum2E571D298AA98110
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U00090 EMBL· GenBank· DDBJ | AAB91603.1 EMBL· GenBank· DDBJ | Genomic DNA |