P55073 · IOD3_HUMAN
- ProteinThyroxine 5-deiodinase
- GeneDIO3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids304 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.
Catalytic activity
- 3,3',5'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
4.3 nM | L-thyroxine |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
1.4 pmol/min/mg |
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 170 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endosome membrane | |
Cellular Component | intracellular membrane-bounded organelle | |
Cellular Component | plasma membrane | |
Molecular Function | thyroxine 5'-deiodinase activity | |
Molecular Function | thyroxine 5-deiodinase activity | |
Biological Process | brown fat cell proliferation | |
Biological Process | hormone biosynthetic process | |
Biological Process | positive regulation of multicellular organism growth | |
Biological Process | response to hypoxia | |
Biological Process | retinal cone cell apoptotic process | |
Biological Process | retinal cone cell development | |
Biological Process | thyroid hormone catabolic process | |
Biological Process | thyroid hormone metabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThyroxine 5-deiodinase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionP55073
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Single-pass type II membrane protein
Endosome membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Topological domain | 1-44 | Cytoplasmic | |||
Transmembrane | 45-67 | Helical; Signal-anchor for type II membrane protein | |||
Topological domain | 68-304 | Extracellular | |||
Keywords
- Cellular component
Disease & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 170 | Complete loss of activity. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 346 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000154323 | 1-304 | Thyroxine 5-deiodinase | ||
Proteomic databases
PTM databases
Structure
Sequence
- Sequence statusComplete
- Length304
- Mass (Da)33,947
- Last updated2014-05-14 v4
- MD5 Checksum40A1B8AA6D633FC8314F9F4F210148D3
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8V8TPY2 | A0A8V8TPY2_HUMAN | DIO3 | 131 |
Sequence caution
Features
Showing features for sequence conflict, non-standard residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 29 | in Ref. 1; AAB35616 | |||
Sequence conflict | 79 | in Ref. 1; AAB35616 | |||
Non-standard residue | 170 | Selenocysteine | |||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
S79854 EMBL· GenBank· DDBJ | AAB35616.2 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AL049836 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC017717 EMBL· GenBank· DDBJ | AAH17717.2 EMBL· GenBank· DDBJ | mRNA | Different initiation |