P54830 · PTN5_MOUSE
- ProteinTyrosine-protein phosphatase non-receptor type 5
- GenePtpn5
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids541 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.
Catalytic activity
- H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 437 | substrate | ||||
Sequence: D | ||||||
Active site | 472 | Phosphocysteine intermediate | ||||
Sequence: C | ||||||
Binding site | 472-478 | substrate | ||||
Sequence: CSAGIGR | ||||||
Binding site | 516 | substrate | ||||
Sequence: Q |
GO annotations
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTyrosine-protein phosphatase non-receptor type 5
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP54830
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Isoform STEP61
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Isoform STEP46
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 64-84 | Helical | ||||
Sequence: LCLFAASQFLLACGVLWLSGH | ||||||
Transmembrane | 122-142 | Helical | ||||
Sequence: LLLVSLTVSLVIVTTLVWHLL |
Keywords
- Cellular component
Phenotypes & Variants
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 21 variants from UniProt as well as other sources including ClinVar and dbSNP.
Chemistry
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000094756 | 1-541 | Tyrosine-protein phosphatase non-receptor type 5 | |||
Sequence: MCCSERLLGLPQPVEMEAPDEAEGLPSKQKEMPPPPPPSPPSEPAQKLPPQGAGSHSLTVRSSLCLFAASQFLLACGVLWLSGHGHSWLQNTTDLISSSLTVLNHLGPVAWLGSGTWGIPSLLLVSLTVSLVIVTTLVWHLLKAPPEPPAPLPPEDRRQSVSRQPSFTYSEWMEEKVEDDFLDLDAVPETPVFDCVMDIKPETDPASLTVKSMGLQERRGSNVSLTLDMCTPGCNEEGFGYLVSPREESAHEYLLSASRVLRAEELHEKALDPFLLQAEFFEIPMNFVDPKEYDIPGLVRKNRYKTILPNPHSRVRLTSPDPEDPLSSYINANYIRGYSGEEKVYIATQGPIVSTVADFWRMVWQERTPIIVMITNIEEMNEKCTEYWPEEQVVHDGVEITVQKVIHTEDYRLRLISLRRGTEERSLKHYWFTSWPDQKTPDRAPPLLHLVREVEEAAQQEGPHCSPIIVHCSAGIGRTGCFIATSICCQQLRREGVVDILKTTCQLRQDRGGMIQTCEQYQFVHHAMSLYEKQLSLQSSE | ||||||
Modified residue | 221 | Phosphoserine; by PKA | ||||
Sequence: S | ||||||
Modified residue | 231 | Phosphothreonine; by MAPK | ||||
Sequence: T | ||||||
Modified residue | 244 | Phosphoserine; by MAPK | ||||
Sequence: S |
Post-translational modification
Phosphorylation at Ser-221 by PKA deactivates PTPN5. Phosphorylation at Thr-231 and Ser-244 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase (By similarity).
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
XENO | P54830-1 | MAPK14 Q16539-1 | 6 | EBI-16067443, EBI-15834191 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-55 | Disordered | ||||
Sequence: MCCSERLLGLPQPVEMEAPDEAEGLPSKQKEMPPPPPPSPPSEPAQKLPPQGAGS | ||||||
Compositional bias | 29-47 | Pro residues | ||||
Sequence: QKEMPPPPPPSPPSEPAQK | ||||||
Domain | 276-531 | Tyrosine-protein phosphatase | ||||
Sequence: LQAEFFEIPMNFVDPKEYDIPGLVRKNRYKTILPNPHSRVRLTSPDPEDPLSSYINANYIRGYSGEEKVYIATQGPIVSTVADFWRMVWQERTPIIVMITNIEEMNEKCTEYWPEEQVVHDGVEITVQKVIHTEDYRLRLISLRRGTEERSLKHYWFTSWPDQKTPDRAPPLLHLVREVEEAAQQEGPHCSPIIVHCSAGIGRTGCFIATSICCQQLRREGVVDILKTTCQLRQDRGGMIQTCEQYQFVHHAMSLY |
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 4 isoforms produced by Alternative splicing. Additional isoforms seem to exist.
P54830-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameSTEP61
- Length541
- Mass (Da)60,815
- Last updated2009-02-10 v2
- Checksum31A0D2C9139F90A4
P54830-2
- NameSTEP46
- Differences from canonical
- 1-172: Missing
P54830-3
- NameSTEP38
- NoteLacks the catalytic domain.
P54830-4
- NameSTEP20
- NoteLacks the catalytic domain.
Features
Showing features for alternative sequence, compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_005126 | 1-172 | in isoform STEP20 and isoform STEP46 | |||
Sequence: Missing | ||||||
Compositional bias | 29-47 | Pro residues | ||||
Sequence: QKEMPPPPPPSPPSEPAQK | ||||||
Alternative sequence | VSP_005127 | 337-346 | in isoform STEP38 and isoform STEP20 | |||
Sequence: GYSGEEKVYI → VCSSIPRAFH | ||||||
Alternative sequence | VSP_005128 | 347-541 | in isoform STEP38 and isoform STEP20 | |||
Sequence: Missing | ||||||
Sequence conflict | 426 | in Ref. 1; AAA73574 | ||||
Sequence: S → T |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U28217 EMBL· GenBank· DDBJ | AAA73574.1 EMBL· GenBank· DDBJ | mRNA | ||
U28216 EMBL· GenBank· DDBJ | AAA73573.1 EMBL· GenBank· DDBJ | mRNA | ||
S80329 EMBL· GenBank· DDBJ | AAB35656.2 EMBL· GenBank· DDBJ | mRNA | ||
AK038416 EMBL· GenBank· DDBJ | BAC29993.1 EMBL· GenBank· DDBJ | mRNA | ||
CH466603 EMBL· GenBank· DDBJ | EDL22958.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC079592 EMBL· GenBank· DDBJ | AAH79592.1 EMBL· GenBank· DDBJ | mRNA |