P54679 · PMA1_DICDI
- ProteinProbable plasma membrane ATPase
- GenepatB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1058 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
P-type plasma membrane H+-ATPase (proton pump) (PubMed:9421912).
The proton gradient it generates drives the active transport of nutrients by H+ symport (PubMed:9421912).
The resulting external acidification and/or internal alkinization may mediate growth responses (PubMed:9421912).
The proton gradient it generates drives the active transport of nutrients by H+ symport (PubMed:9421912).
The resulting external acidification and/or internal alkinization may mediate growth responses (PubMed:9421912).
Catalytic activity
- ATP + H+(in) + H2O = ADP + 2 H+(out) + phosphateThis reaction proceeds in the forward direction.
CHEBI:30616 + H+ (in)CHEBI:15378+ CHEBI:15377 = CHEBI:456216 + 2 H+ (out)CHEBI:15378+ CHEBI:43474
Activity regulation
Acid pH levels increase its ATPase activity.
Features
Showing features for active site, binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | pathogen-containing vacuole | |
Cellular Component | plasma membrane | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | metal ion binding | |
Molecular Function | P-type proton-exporting transporter activity | |
Biological Process | proton export across plasma membrane | |
Biological Process | proton transmembrane transport | |
Biological Process | regulation of intracellular pH |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable plasma membrane ATPase
- EC number
- Short namesPAT2
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Amoebozoa > Evosea > Eumycetozoa > Dictyostelia > Dictyosteliales > Dictyosteliaceae > Dictyostelium
Accessions
- Primary accessionP54679
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-212 | Cytoplasmic | ||||
Sequence: MDNNQIPKNSPESSAINSAESSPKSNVSSDVLHENHHKEQQQLQQQLQQEQQQQQLPTTPQSEPTQRVNNNEEGIEMDRIAENSNINVPSDVGESSLKTISGYPSSKNTEAGSSSSGKKEEDYNYRSTWVPKLHQELFENPEVLESRRTKQRASNYRKTLEREKEGIKPLDNILEELKANANGLTKAEAQKRLEEVGPNAIPDVKRYPILEF | ||||||
Transmembrane | 213-232 | Helical | ||||
Sequence: LYFMWNPLSWTMEVAAIVSI | ||||||
Topological domain | 233-237 | Extracellular | ||||
Sequence: ALLDW | ||||||
Transmembrane | 238-258 | Helical | ||||
Sequence: VDFILICALLLLNATIGFIEE | ||||||
Topological domain | 259-387 | Cytoplasmic | ||||
Sequence: NTAGNAVEALKNSLVSQIRCMRDGEWVMLPSPDLVPGDVVMLKIGAIIPADCRVIEAEQVKIDQSSLTGESLPVTKKIGDEVYSGSAMKQGEAKCVVTATGVNTFFGRAANLVQETEGHGHLQVILRNI | ||||||
Transmembrane | 388-407 | Helical | ||||
Sequence: GLFCISFIAIWVLVELLVDF | ||||||
Topological domain | 408-425 | Extracellular | ||||
Sequence: LGYDGYCHGVGGGRCLPL | ||||||
Transmembrane | 426-447 | Helical | ||||
Sequence: NNALVLLVGGIPIAMPTVLSVT | ||||||
Topological domain | 448-783 | Cytoplasmic | ||||
Sequence: MAIGATQLSKKKAIVSRLASIEELAAMDILCSDKTGTLTLNILTVDEPLPVGDTPKEDIVFHAFLACSEGEDQDAIDKAISNYCRDTYPNVDYSGNEIVKHYPFNPEDKKAMGLVNANGKQFKTAKGAPQIILREADNYKQVGEAVEKEIENLADRGYRALGVSVSYDAPDFKVWHFEGLIPLFDPPRHDTEDTIKRALEMGVSVKMITGDQLAIAKETARRLGMGGNLFTIPYLENNDLGISEGEVIEMADGFAEMWPEHKYKVVDQLQKRKHVVGMTGDGVNDAPALKKAQIGIAVAGATDAARSVSDIVLTSSGLSVIIDAIISSRKIFQRMR | ||||||
Transmembrane | 784-805 | Helical | ||||
Sequence: NYVIYSVAATVRICTTFGILTV | ||||||
Topological domain | 806-810 | Extracellular | ||||
Sequence: AWNFK | ||||||
Transmembrane | 811-833 | Helical | ||||
Sequence: FPTIATVIIAILNDGTMLTISKD | ||||||
Topological domain | 834-849 | Cytoplasmic | ||||
Sequence: RVRARNEPDQWNLFEV | ||||||
Transmembrane | 850-870 | Helical | ||||
Sequence: FTMALCYGFYLVGSTIVFFAI | ||||||
Topological domain | 871-889 | Extracellular | ||||
Sequence: IHDGTWFHDAINLRILTDN | ||||||
Transmembrane | 890-910 | Helical | ||||
Sequence: ELRGLIYLQVSISGLATIFVS | ||||||
Topological domain | 911-922 | Cytoplasmic | ||||
Sequence: RSQGFSYFERPG | ||||||
Transmembrane | 923-943 | Helical | ||||
Sequence: NLVIFAFVMSQIVATFIGVYG | ||||||
Topological domain | 944-967 | Extracellular | ||||
Sequence: FRGYPHDSFSDNPDYPVHGTNFQG | ||||||
Transmembrane | 968-988 | Helical | ||||
Sequence: CGWGWAVCAWIWCFLWYIPMD | ||||||
Topological domain | 989-1058 | Cytoplasmic | ||||
Sequence: FIKLGVTYILRGKIEPINKDALRKIYGWFGKEIPKEATQVSHKVAEQQAKRDALHAQETHHKSVVTDNKV |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000046295 | 1-1058 | Probable plasma membrane ATPase | |||
Sequence: MDNNQIPKNSPESSAINSAESSPKSNVSSDVLHENHHKEQQQLQQQLQQEQQQQQLPTTPQSEPTQRVNNNEEGIEMDRIAENSNINVPSDVGESSLKTISGYPSSKNTEAGSSSSGKKEEDYNYRSTWVPKLHQELFENPEVLESRRTKQRASNYRKTLEREKEGIKPLDNILEELKANANGLTKAEAQKRLEEVGPNAIPDVKRYPILEFLYFMWNPLSWTMEVAAIVSIALLDWVDFILICALLLLNATIGFIEENTAGNAVEALKNSLVSQIRCMRDGEWVMLPSPDLVPGDVVMLKIGAIIPADCRVIEAEQVKIDQSSLTGESLPVTKKIGDEVYSGSAMKQGEAKCVVTATGVNTFFGRAANLVQETEGHGHLQVILRNIGLFCISFIAIWVLVELLVDFLGYDGYCHGVGGGRCLPLNNALVLLVGGIPIAMPTVLSVTMAIGATQLSKKKAIVSRLASIEELAAMDILCSDKTGTLTLNILTVDEPLPVGDTPKEDIVFHAFLACSEGEDQDAIDKAISNYCRDTYPNVDYSGNEIVKHYPFNPEDKKAMGLVNANGKQFKTAKGAPQIILREADNYKQVGEAVEKEIENLADRGYRALGVSVSYDAPDFKVWHFEGLIPLFDPPRHDTEDTIKRALEMGVSVKMITGDQLAIAKETARRLGMGGNLFTIPYLENNDLGISEGEVIEMADGFAEMWPEHKYKVVDQLQKRKHVVGMTGDGVNDAPALKKAQIGIAVAGATDAARSVSDIVLTSSGLSVIIDAIISSRKIFQRMRNYVIYSVAATVRICTTFGILTVAWNFKFPTIATVIIAILNDGTMLTISKDRVRARNEPDQWNLFEVFTMALCYGFYLVGSTIVFFAIIHDGTWFHDAINLRILTDNELRGLIYLQVSISGLATIFVSRSQGFSYFERPGNLVIFAFVMSQIVATFIGVYGFRGYPHDSFSDNPDYPVHGTNFQGCGWGWAVCAWIWCFLWYIPMDFIKLGVTYILRGKIEPINKDALRKIYGWFGKEIPKEATQVSHKVAEQQAKRDALHAQETHHKSVVTDNKV |
Keywords
- PTM
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-29 | Polar residues | ||||
Sequence: MDNNQIPKNSPESSAINSAESSPKSNVSS | ||||||
Region | 1-123 | Disordered | ||||
Sequence: MDNNQIPKNSPESSAINSAESSPKSNVSSDVLHENHHKEQQQLQQQLQQEQQQQQLPTTPQSEPTQRVNNNEEGIEMDRIAENSNINVPSDVGESSLKTISGYPSSKNTEAGSSSSGKKEEDY | ||||||
Compositional bias | 41-71 | Polar residues | ||||
Sequence: QQLQQQLQQEQQQQQLPTTPQSEPTQRVNNN | ||||||
Compositional bias | 83-113 | Polar residues | ||||
Sequence: NSNINVPSDVGESSLKTISGYPSSKNTEAGS |
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,058
- Mass (Da)117,268
- Last updated2007-12-04 v2
- Checksum12D39896E92F3A9F
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-29 | Polar residues | ||||
Sequence: MDNNQIPKNSPESSAINSAESSPKSNVSS | ||||||
Compositional bias | 41-71 | Polar residues | ||||
Sequence: QQLQQQLQQEQQQQQLPTTPQSEPTQRVNNN | ||||||
Compositional bias | 83-113 | Polar residues | ||||
Sequence: NSNINVPSDVGESSLKTISGYPSSKNTEAGS |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X98286 EMBL· GenBank· DDBJ | CAA66931.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AAFI02000047 EMBL· GenBank· DDBJ | EAL65988.1 EMBL· GenBank· DDBJ | Genomic DNA |