P54144 · AMT11_ARATH
- ProteinAmmonium transporter 1 member 1
- GeneAMT1-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids501 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
High affinity ammonium transporter probably involved in ammonium uptake from the soil, long-distance transport to the shoots and re-uptake of apoplastic ammonium that derives from photorespiration in shoots. Contributes with AMT1-3 to the overall ammonium uptake capacity in roots under nitrogen-deficiency conditions.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
22 μM | ammonium chloride | 6.1 | external |
Measured in yeast knockout mutant YCW012.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | ammonium transmembrane transporter complex | |
Cellular Component | plasma membrane | |
Cellular Component | plasmodesma | |
Molecular Function | ammonium channel activity | |
Biological Process | ammonium transmembrane transport | |
Biological Process | lateral root branching | |
Biological Process | lateral root formation | |
Biological Process | protein polymerization |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameAmmonium transporter 1 member 1
- Short namesAtAMT1;1
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionP54144
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 8-28 | Helical | ||||
Sequence: LAVLLGPNATAAANYICGQLG | ||||||
Transmembrane | 46-66 | Helical | ||||
Sequence: LLFSAYLVFSMQLGFAMLCAG | ||||||
Transmembrane | 81-101 | Helical | ||||
Sequence: VLDAAAGGLFYYLFGYAFAFG | ||||||
Transmembrane | 128-148 | Helical | ||||
Sequence: FLYQWAFAIAAAGITSGSIAE | ||||||
Transmembrane | 152-172 | Helical | ||||
Sequence: FVAYLIYSSFLTGFVYPVVSH | ||||||
Transmembrane | 199-219 | Helical | ||||
Sequence: FAGSGVVHMVGGIAGLWGALI | ||||||
Transmembrane | 243-263 | Helical | ||||
Sequence: LVVLGTFLLWFGWYGFNPGSF | ||||||
Transmembrane | 333-353 | Helical | ||||
Sequence: VVEPWAAIICGFVAALVLLGC | ||||||
Transmembrane | 366-386 | Helical | ||||
Sequence: LEAAQLHGGCGAWGLIFTALF | ||||||
Transmembrane | 419-439 | Helical | ||||
Sequence: LIQIIVITGWVSATMGTLFFI |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
No effect on ammonium uptake. Higher expression of AMT1-2; AMT1-3 and AMT2-1.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 11 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000224180 | 1-501 | Ammonium transporter 1 member 1 | |||
Sequence: MSCSATDLAVLLGPNATAAANYICGQLGDVNNKFIDTAFAIDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAFGSPSNGFIGKHYFGLKDIPTASADYSNFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFVYPVVSHWFWSVDGWASPFRTDGDLLFSTGAIDFAGSGVVHMVGGIAGLWGALIEGPRLGRFDNGGRAIALRGHSASLVVLGTFLLWFGWYGFNPGSFNKILVTYETGTYNGQWSAVGRTAVTTTLAGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITGGCSVVEPWAAIICGFVAALVLLGCNKLAEKLKYDDPLEAAQLHGGCGAWGLIFTALFAQEKYLNQIYGNKPGRPHGLFMGGGGKLLGAQLIQIIVITGWVSATMGTLFFILKKMKLLRISSEDEMAGMDMTRHGGFAYMYFDDDESHKAIQLRRVEPRSPSPSGANTTPTPV | ||||||
Modified residue | 460 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 475 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 488 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 490 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 492 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Highly expressed in roots. Expressed in root tips, root hairs, root epidermis, rhizodermis, cortex and pericycle. Expressed in leaves epidermal and mesophyll cells.
Induction
By nitrogen deprivation in roots and nitrogen supply in leaves.
Gene expression databases
Interaction
Subunit
Self interacts. Interacts with the receptor protein kinases CEPR2, At2g28990 and PAM74.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P54144 | AMT1-3 Q9SQH9 | 3 | EBI-16426081, EBI-16716530 |
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length501
- Mass (Da)53,577
- Last updated1996-10-01 v1
- Checksum070BAF8228302BFF
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X75879 EMBL· GenBank· DDBJ | CAA53473.1 EMBL· GenBank· DDBJ | mRNA | ||
AL049656 EMBL· GenBank· DDBJ | CAB41109.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL161536 EMBL· GenBank· DDBJ | CAB78393.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002687 EMBL· GenBank· DDBJ | AEE83287.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY037219 EMBL· GenBank· DDBJ | AAK59819.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AY113167 EMBL· GenBank· DDBJ | AAM47470.1 EMBL· GenBank· DDBJ | mRNA | Frameshift |