P54001 · P4HA1_RAT
- ProteinProlyl 4-hydroxylase subunit alpha-1
- GeneP4ha1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids534 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Catalytic activity
- 2-oxoglutarate + L-prolyl-[collagen] + O2 = CO2 + succinate + trans-4-hydroxy-L-prolyl-[collagen]
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Fe2+ ion per subunit.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | endoplasmic reticulum lumen | |
Cellular Component | procollagen-proline 4-dioxygenase complex | |
Molecular Function | identical protein binding | |
Molecular Function | iron ion binding | |
Molecular Function | L-ascorbic acid binding | |
Molecular Function | procollagen-proline 4-dioxygenase activity | |
Biological Process | collagen fibril organization |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProlyl 4-hydroxylase subunit alpha-1
- EC number
- Short names4-PH alpha-1
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionP54001
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Chemistry
PTM/Processing
Features
Showing features for signal, chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-17 | |||||
Sequence: MIWGVLMMGILLPQCSA | ||||||
Chain | PRO_0000022725 | 18-534 | Prolyl 4-hydroxylase subunit alpha-1 | |||
Sequence: HPGFFTSIGQMTDLIHNEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLILKDMSDGFISNLTIQRQYFPNDEDQVGAAKALFRLQDTYNLDTNTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALMQLEEGEMSTVDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLVYFEYIMSKEKDANKSASGDQSDQKTTPKKKGIAVDYLPERQKYEMLCRGEGIKMTPRRQKRLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLSRATVHDPETGKLTTAQYRVSKSAWLSGYEDPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFRELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE | ||||||
Glycosylation | 113 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 259 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Heterotetramer of two alpha-1 chains and two beta chains (P4HB)(the beta chain is the multi-functional PDI), where P4HB plays the role of a structural subunit; this tetramer catalyzes the formation of 4-hydroxyproline in collagen.
Protein-protein interaction databases
Chemistry
Structure
Family & Domains
Features
Showing features for repeat, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Repeat | 205-238 | TPR | ||||
Sequence: VSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEH | ||||||
Domain | 411-519 | Fe2OG dioxygenase | ||||
Sequence: TAEELQVANYGVGGQYEPHFDFARKDEPDAFRELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHE |
Sequence similarities
Belongs to the P4HA family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length534
- Mass (Da)60,898
- Last updated2005-09-13 v2
- ChecksumC44659978281FF30
Computationally mapped potential isoform sequences
There are 5 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8I6AP51 | A0A8I6AP51_RAT | P4ha1 | 525 | ||
A0A8I6ASU1 | A0A8I6ASU1_RAT | P4ha1 | 534 | ||
A0A8L2QWJ8 | A0A8L2QWJ8_RAT | P4ha1 | 528 | ||
A0A8I6A688 | A0A8I6A688_RAT | P4ha1 | 454 | ||
A0A8I5ZKB2 | A0A8I5ZKB2_RAT | P4ha1 | 481 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 265-267 | in Ref. 1; CAA55546 | ||||
Sequence: DQS → ERA |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X78949 EMBL· GenBank· DDBJ | CAA55546.1 EMBL· GenBank· DDBJ | mRNA | ||
BC078703 EMBL· GenBank· DDBJ | AAH78703.1 EMBL· GenBank· DDBJ | mRNA |