P53934 · CARME_YEAST
- ProteinCarnosine N-methyltransferase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids400 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
N-methyltransferase that mediates the formation of anserine (beta-alanyl-N(Pi)-methyl-L-histidine) from carnosine. Also methylates other L-histidine-containing di- and tripeptides such as Gly-Gly-His, Gly-His and homocarnosine (GABA-His).
Miscellaneous
Present with 952 molecules/cell in log phase SD medium.
Catalytic activity
- carnosine + S-adenosyl-L-methionine = anserine + H+ + S-adenosyl-L-homocysteine
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
21.5 mM | carnosine | |||||
0.002 mM | S-adenosyl-L-methionine |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
7.89 nmol/min/mg | with carnosine as substrate |
kcat is 0.38 min-1 for carnosine. kcat is 0.74 min-1 for S-adenosyl-L-methionine.
pH Dependence
Optimum pH is 7.0-7.5.
Temperature Dependence
Optimum temperature is 30 degrees Celsius.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 108 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 111 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 146 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 167 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 235 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: F | ||||||
Binding site | 278 | carnosine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 286 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 309 | carnosine (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 385 | carnosine (UniProtKB | ChEBI) | ||||
Sequence: Y |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | carnosine N-methyltransferase activity | |
Molecular Function | S-adenosylmethionine-dependent methyltransferase activity | |
Biological Process | carnosine metabolic process | |
Biological Process | methylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCarnosine N-methyltransferase
- EC number
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP53934
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 42 | in strain: SK1 | ||||
Sequence: A → E | ||||||
Natural variant | 154 | in strain: SK1 | ||||
Sequence: V → M | ||||||
Natural variant | 317 | in strain: SK1 | ||||
Sequence: E → D |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 14 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000203441 | 1-400 | Carnosine N-methyltransferase | |||
Sequence: MDENEFDNQRENKAVARVIISFLKYEEYALKEIYNLRVKKWASISDRQKDMVPNYTKYLANLKAAIIENGKFFRSVAEYALQSISFEPGEIVQPNDLDMSKTCSLLTQVYREWSAEAISERNCLNSRLVPFLKTLSPPKADILIPGCGTGRLLVDLSRMGYNCEGNEFSYHMLLVSQYMLNAGLLQNQIIIYPFIHCFSHWKKIEDQLSPIKVPDIEAWSSNKGMGSMSICAGSFVDCYGRNQGTKISSHYTFSRRMQLSRAKAENSKDVVVTNFFIDTGSNILDYLDTIGHVLKPGGIWCNFGPLLYHFENDHGVETTYEVNPYSGFQDKINDYTPLMGLELSSDDIISIATNHLDFELIRRESGILCGYGRYAGPESCAMPGYMCHYWILKSNPTNES |
Proteomic databases
Structure
Sequence
- Sequence statusComplete
- Length400
- Mass (Da)45,497
- Last updated1996-10-01 v1
- Checksum6B6C051891F075EE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X85811 EMBL· GenBank· DDBJ | CAA59825.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DQ115393 EMBL· GenBank· DDBJ | AAZ22516.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z71368 EMBL· GenBank· DDBJ | CAA95968.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006947 EMBL· GenBank· DDBJ | DAA10454.1 EMBL· GenBank· DDBJ | Genomic DNA |