P53934 · CARME_YEAST

Function

function

N-methyltransferase that mediates the formation of anserine (beta-alanyl-N(Pi)-methyl-L-histidine) from carnosine. Also methylates other L-histidine-containing di- and tripeptides such as Gly-Gly-His, Gly-His and homocarnosine (GABA-His).

Miscellaneous

Present with 952 molecules/cell in log phase SD medium.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
21.5 mMcarnosine
0.002 mMS-adenosyl-L-methionine
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
7.89 nmol/min/mgwith carnosine as substrate
kcat is 0.38 min-1 for carnosine. kcat is 0.74 min-1 for S-adenosyl-L-methionine.

pH Dependence

Optimum pH is 7.0-7.5.

Temperature Dependence

Optimum temperature is 30 degrees Celsius.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site108S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site111S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site146S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site167S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site235S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site278carnosine (UniProtKB | ChEBI)
Binding site286S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site309carnosine (UniProtKB | ChEBI)
Binding site385carnosine (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functioncarnosine N-methyltransferase activity
Molecular FunctionS-adenosylmethionine-dependent methyltransferase activity
Biological Processcarnosine metabolic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carnosine N-methyltransferase
  • EC number

Gene names

    • ORF names
      N2227
    • Ordered locus names
      YNL092W

Organism names

Accessions

  • Primary accession
    P53934
  • Secondary accessions
    • D6W188
    • Q45TZ4

Proteomes

Organism-specific databases

Phenotypes & Variants

Features

Showing features for natural variant.

TypeIDPosition(s)Description
Natural variant42in strain: SK1
Natural variant154in strain: SK1
Natural variant317in strain: SK1

Variants

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The viewer provides 14 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002034411-400Carnosine N-methyltransferase

Proteomic databases

Interaction

Protein-protein interaction databases

Miscellaneous

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    400
  • Mass (Da)
    45,497
  • Last updated
    1996-10-01 v1
  • Checksum
    6B6C051891F075EE
MDENEFDNQRENKAVARVIISFLKYEEYALKEIYNLRVKKWASISDRQKDMVPNYTKYLANLKAAIIENGKFFRSVAEYALQSISFEPGEIVQPNDLDMSKTCSLLTQVYREWSAEAISERNCLNSRLVPFLKTLSPPKADILIPGCGTGRLLVDLSRMGYNCEGNEFSYHMLLVSQYMLNAGLLQNQIIIYPFIHCFSHWKKIEDQLSPIKVPDIEAWSSNKGMGSMSICAGSFVDCYGRNQGTKISSHYTFSRRMQLSRAKAENSKDVVVTNFFIDTGSNILDYLDTIGHVLKPGGIWCNFGPLLYHFENDHGVETTYEVNPYSGFQDKINDYTPLMGLELSSDDIISIATNHLDFELIRRESGILCGYGRYAGPESCAMPGYMCHYWILKSNPTNES

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X85811
EMBL· GenBank· DDBJ
CAA59825.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ115393
EMBL· GenBank· DDBJ
AAZ22516.1
EMBL· GenBank· DDBJ
Genomic DNA
Z71368
EMBL· GenBank· DDBJ
CAA95968.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006947
EMBL· GenBank· DDBJ
DAA10454.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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