P53612 · PGTB2_MOUSE
- ProteinGeranylgeranyl transferase type-2 subunit beta
- GeneRabggtb
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids339 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of Rab proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX, such as RAB1A, RAB3A, RAB5A and RAB7A.
Catalytic activity
- geranylgeranyl diphosphate + L-cysteinyl-[protein] = diphosphate + S-geranylgeranyl-L-cysteinyl-[protein]
Cofactor
Note: Binds 1 zinc ion per subunit.
Activity regulation
The enzymatic reaction requires the aid of a Rab escort protein (also called component A), such as CHM.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 198-200 | geranylgeranyl diphosphate (UniProtKB | ChEBI) | ||||
Sequence: HAG | ||||||
Binding site | 240-243 | geranylgeranyl diphosphate (UniProtKB | ChEBI) | ||||
Sequence: RPEK | ||||||
Binding site | 246 | Zn2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 248 | Zn2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: C | ||||||
Binding site | 249 | geranylgeranyl diphosphate (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 249-252 | geranylgeranyl diphosphate (UniProtKB | ChEBI) | ||||
Sequence: YSWW | ||||||
Binding site | 298 | Zn2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Rab-protein geranylgeranyltransferase complex | |
Molecular Function | isoprenoid binding | |
Molecular Function | Rab geranylgeranyltransferase activity | |
Molecular Function | small GTPase binding | |
Molecular Function | zinc ion binding | |
Biological Process | protein geranylgeranylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGeranylgeranyl transferase type-2 subunit beta
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP53612
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000119773 | 1-339 | Geranylgeranyl transferase type-2 subunit beta | |||
Sequence: MGSLLFSWKGTQQKDVTIKSDAPDTLLLEKHADYIASYGSKKDDYEYCMSEYLRMSGVYWGLTVMDLMGQLHRMNREEILVFIKSCQHECGGISASIGHDPHLLYTLSAVQILTLYDSVHVINVDKVVAYVQSLQKEDGSFAGDIWGEIDTRFSFCAVATLALLGKLDAINVEKAIEFVLSCMNFDGGFGCRPGSESHAGQIYCCTGFLAITSQLHQVNSDLLGWWLCERQLPSGGLNGRPEKLPDVCYSWWVLASLKIIGRLHWIDREKLRSFILACQDEETGGFADRPGDMVDPFHTLFGIAGLSLLGEEQIKPVSPVFCMPEEVLQRVNVQPELVS | ||||||
Modified residue | 11 | Phosphothreonine | ||||
Sequence: T |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Ubiquitous. Detected in all the major organs in adult animals.
Induction
Increased dramatically by cycloheximide (CHX) treatment within a short time (as early as 2 hours). Actinomycin D was used to determine the half-life, CHX treatment resulted in a dramatic increase of the half-life from 8 hours to greater than 12 hours.
Developmental stage
Specific expression was elevated in mid-gestation stages, particularly developing liver and spinal cord.
Gene expression databases
Interaction
Subunit
Heterotrimer composed of RABGGTA, RABGGTB and CHM; within this trimer, RABGGTA and RABGGTB form the catalytic component B, while CHM (component A) mediates peptide substrate binding (PubMed:21520375).
The Rab GGTase dimer (RGGT) interacts with CHM (component A) prior to Rab protein binding; the association is stabilized by geranylgeranyl pyrophosphate (GGpp) (By similarity).
The CHM:RGGT:Rab complex is destabilized by GGpp (By similarity).
Interaction of RABGGTB with prenylated PTP4A2 precludes its association with RABGGTA and inhibits enzyme activity (By similarity).
Interacts with CHODL (PubMed:18161010).
Interacts with non-phosphorylated form of RAB8A; phosphorylation of RAB8A at 'Thr-72' disrupts this interaction (By similarity).
The Rab GGTase dimer (RGGT) interacts with CHM (component A) prior to Rab protein binding; the association is stabilized by geranylgeranyl pyrophosphate (GGpp) (By similarity).
The CHM:RGGT:Rab complex is destabilized by GGpp (By similarity).
Interaction of RABGGTB with prenylated PTP4A2 precludes its association with RABGGTA and inhibits enzyme activity (By similarity).
Interacts with CHODL (PubMed:18161010).
Interacts with non-phosphorylated form of RAB8A; phosphorylation of RAB8A at 'Thr-72' disrupts this interaction (By similarity).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P53612 | Chodl Q9CXM0 | 2 | EBI-9104297, EBI-13948582 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Repeat | 28-69 | PFTB 1 | ||||
Sequence: LEKHADYIASYGSKKDDYEYCMSEYLRMSGVYWGLTVMDLMG | ||||||
Repeat | 76-117 | PFTB 2 | ||||
Sequence: REEILVFIKSCQHECGGISASIGHDPHLLYTLSAVQILTLYD | ||||||
Repeat | 124-165 | PFTB 3 | ||||
Sequence: VDKVVAYVQSLQKEDGSFAGDIWGEIDTRFSFCAVATLALLG | ||||||
Repeat | 172-213 | PFTB 4 | ||||
Sequence: VEKAIEFVLSCMNFDGGFGCRPGSESHAGQIYCCTGFLAITS | ||||||
Repeat | 220-261 | PFTB 5 | ||||
Sequence: SDLLGWWLCERQLPSGGLNGRPEKLPDVCYSWWVLASLKIIG | ||||||
Repeat | 268-310 | PFTB 6 | ||||
Sequence: REKLRSFILACQDEETGGFADRPGDMVDPFHTLFGIAGLSLLG |
Sequence similarities
Belongs to the protein prenyltransferase subunit beta family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length339
- Mass (Da)37,803
- Last updated2011-07-27 v2
- Checksum4765A39E5392B8B5
Computationally mapped potential isoform sequences
There are 9 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q9JIF6 | Q9JIF6_MOUSE | Rabggtb | 153 | ||
Q3TVF4 | Q3TVF4_MOUSE | Rabggtb | 331 | ||
Q6PFA1 | Q6PFA1_MOUSE | Rabggtb | 132 | ||
A0A0G2JG79 | A0A0G2JG79_MOUSE | Rabggtb | 205 | ||
A0A0G2JFP7 | A0A0G2JFP7_MOUSE | Rabggtb | 59 | ||
A0A0G2JGH5 | A0A0G2JGH5_MOUSE | Rabggtb | 80 | ||
A0A0G2JGU4 | A0A0G2JGU4_MOUSE | Rabggtb | 152 | ||
A0A0G2JDK3 | A0A0G2JDK3_MOUSE | Rabggtb | 149 | ||
A0A0G2JF29 | A0A0G2JF29_MOUSE | Rabggtb | 162 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 230 | in Ref. 1; AAB01502 | ||||
Sequence: R → A | ||||||
Sequence conflict | 236 | in Ref. 1; AAB01502 | ||||
Sequence: G → A | ||||||
Sequence conflict | 241 | in Ref. 1; AAB01502 | ||||
Sequence: P → R | ||||||
Sequence conflict | 267-268 | in Ref. 1; AAB01502 | ||||
Sequence: DR → VS |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U12922 EMBL· GenBank· DDBJ | AAB01502.1 EMBL· GenBank· DDBJ | mRNA | ||
CH466532 EMBL· GenBank· DDBJ | EDL11902.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC132473 EMBL· GenBank· DDBJ | AAI32474.1 EMBL· GenBank· DDBJ | mRNA | ||
BC138547 EMBL· GenBank· DDBJ | AAI38548.1 EMBL· GenBank· DDBJ | mRNA |