P53612 · PGTB2_MOUSE

  • Protein
    Geranylgeranyl transferase type-2 subunit beta
  • Gene
    Rabggtb
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of Rab proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX, such as RAB1A, RAB3A, RAB5A and RAB7A.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Activity regulation

The enzymatic reaction requires the aid of a Rab escort protein (also called component A), such as CHM.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site198-200geranylgeranyl diphosphate (UniProtKB | ChEBI)
Binding site240-243geranylgeranyl diphosphate (UniProtKB | ChEBI)
Binding site246Zn2+ (UniProtKB | ChEBI); catalytic
Binding site248Zn2+ (UniProtKB | ChEBI); catalytic
Binding site249geranylgeranyl diphosphate (UniProtKB | ChEBI)
Binding site249-252geranylgeranyl diphosphate (UniProtKB | ChEBI)
Binding site298Zn2+ (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Cellular ComponentRab-protein geranylgeranyltransferase complex
Molecular Functionisoprenoid binding
Molecular FunctionRab geranylgeranyltransferase activity
Molecular Functionsmall GTPase binding
Molecular Functionzinc ion binding
Biological Processprotein geranylgeranylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Geranylgeranyl transferase type-2 subunit beta
  • EC number
  • Alternative names
    • Geranylgeranyl transferase type II subunit beta (GGTase-II-beta)
    • Rab geranyl-geranyltransferase subunit beta (Rab GG transferase beta; Rab GGTase beta)
    • Rab geranylgeranyltransferase subunit beta
    • Type II protein geranyl-geranyltransferase subunit beta

Gene names

    • Name
      Rabggtb

Organism names

  • Taxonomic identifier
  • Strain
    • CD-1
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P53612
  • Secondary accessions
    • A2RTE6

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00001197731-339Geranylgeranyl transferase type-2 subunit beta
Modified residue11Phosphothreonine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Ubiquitous. Detected in all the major organs in adult animals.

Induction

Increased dramatically by cycloheximide (CHX) treatment within a short time (as early as 2 hours). Actinomycin D was used to determine the half-life, CHX treatment resulted in a dramatic increase of the half-life from 8 hours to greater than 12 hours.

Developmental stage

Specific expression was elevated in mid-gestation stages, particularly developing liver and spinal cord.

Gene expression databases

Interaction

Subunit

Heterotrimer composed of RABGGTA, RABGGTB and CHM; within this trimer, RABGGTA and RABGGTB form the catalytic component B, while CHM (component A) mediates peptide substrate binding (PubMed:21520375).
The Rab GGTase dimer (RGGT) interacts with CHM (component A) prior to Rab protein binding; the association is stabilized by geranylgeranyl pyrophosphate (GGpp) (By similarity).
The CHM:RGGT:Rab complex is destabilized by GGpp (By similarity).
Interaction of RABGGTB with prenylated PTP4A2 precludes its association with RABGGTA and inhibits enzyme activity (By similarity).
Interacts with CHODL (PubMed:18161010).
Interacts with non-phosphorylated form of RAB8A; phosphorylation of RAB8A at 'Thr-72' disrupts this interaction (By similarity).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P53612Chodl Q9CXM02EBI-9104297, EBI-13948582
View interactors in UniProtKB
View CPX-2920 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for repeat.

TypeIDPosition(s)Description
Repeat28-69PFTB 1
Repeat76-117PFTB 2
Repeat124-165PFTB 3
Repeat172-213PFTB 4
Repeat220-261PFTB 5
Repeat268-310PFTB 6

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    339
  • Mass (Da)
    37,803
  • Last updated
    2011-07-27 v2
  • Checksum
    4765A39E5392B8B5
MGSLLFSWKGTQQKDVTIKSDAPDTLLLEKHADYIASYGSKKDDYEYCMSEYLRMSGVYWGLTVMDLMGQLHRMNREEILVFIKSCQHECGGISASIGHDPHLLYTLSAVQILTLYDSVHVINVDKVVAYVQSLQKEDGSFAGDIWGEIDTRFSFCAVATLALLGKLDAINVEKAIEFVLSCMNFDGGFGCRPGSESHAGQIYCCTGFLAITSQLHQVNSDLLGWWLCERQLPSGGLNGRPEKLPDVCYSWWVLASLKIIGRLHWIDREKLRSFILACQDEETGGFADRPGDMVDPFHTLFGIAGLSLLGEEQIKPVSPVFCMPEEVLQRVNVQPELVS

Computationally mapped potential isoform sequences

There are 9 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
Q9JIF6Q9JIF6_MOUSERabggtb153
Q3TVF4Q3TVF4_MOUSERabggtb331
Q6PFA1Q6PFA1_MOUSERabggtb132
A0A0G2JG79A0A0G2JG79_MOUSERabggtb205
A0A0G2JFP7A0A0G2JFP7_MOUSERabggtb59
A0A0G2JGH5A0A0G2JGH5_MOUSERabggtb80
A0A0G2JGU4A0A0G2JGU4_MOUSERabggtb152
A0A0G2JDK3A0A0G2JDK3_MOUSERabggtb149
A0A0G2JF29A0A0G2JF29_MOUSERabggtb162

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict230in Ref. 1; AAB01502
Sequence conflict236in Ref. 1; AAB01502
Sequence conflict241in Ref. 1; AAB01502
Sequence conflict267-268in Ref. 1; AAB01502

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U12922
EMBL· GenBank· DDBJ
AAB01502.1
EMBL· GenBank· DDBJ
mRNA
CH466532
EMBL· GenBank· DDBJ
EDL11902.1
EMBL· GenBank· DDBJ
Genomic DNA
BC132473
EMBL· GenBank· DDBJ
AAI32474.1
EMBL· GenBank· DDBJ
mRNA
BC138547
EMBL· GenBank· DDBJ
AAI38548.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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