P53555 · BIOK_BACSU
- ProteinL-Lysine--8-amino-7-oxononanoate transaminase
- GenebioK
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids448 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the transfer of the alpha-amino group from L-lysine to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). B.subtilis is the only bacterium known to utilize L-lysine as an amino donor in the biosynthesis of DAPA.
Catalytic activity
- (8S)-8-amino-7-oxononanoate + L-lysine = (7R,8S)-7,8-diammoniononanoate + (S)-2-amino-6-oxohexanoate
Cofactor
Activity regulation
Inhibited by 7-keto-8-aminopelargonic acid at concentrations above 80 uM.
pH Dependence
Optimum pH is 8.6.
Pathway
Cofactor biosynthesis; biotin biosynthesis; 7,8-diaminononanoate from 8-amino-7-oxononanoate (Lys route).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 113-114 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | ||||
Sequence: GA | ||||||
Binding site | 146 | substrate | ||||
Sequence: Y | ||||||
Binding site | 251 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 315 | substrate | ||||
Sequence: G | ||||||
Binding site | 316-317 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | ||||
Sequence: HS | ||||||
Binding site | 410 | substrate | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | adenosylmethionine-8-amino-7-oxononanoate transaminase activity | |
Molecular Function | pyridoxal phosphate binding | |
Biological Process | biotin biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameL-Lysine--8-amino-7-oxononanoate transaminase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus
Accessions
- Primary accessionP53555
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000120361 | 1-448 | L-Lysine--8-amino-7-oxononanoate transaminase | |||
Sequence: MTHDLIEKSKKHLWLPFTQMKDYDENPLIIESGTGIKVKDINGKEYYDGFSSVWLNVHGHRKKELDDAIKKQLGKIAHSTLLGMTNVPATQLAETLIDISPKKLTRVFYSDSGAEAMEIALKMAFQYWKNIGKPEKQKFIAMKNGYHGDTIGAVSVGSIELFHHVYGPLMFESYKAPIPYVYRSESGDPDECRDQCLRELAQLLEEHHEEIAALSIESMVQGASGMIVMPEGYLAGVRELCTTYDVLMIVDEVATGFGRTGKMFACEHENVQPDLMAAGKGITGGYLPIAVTFATEDIYKAFYDDYENLKTFFHGHSYTGNQLGCAVALENLALFESENIVEQVAEKSKKLHFLLQDLHALPHVGDIRQLGFMCGAELVRSKETKEPYPADRRIGYKVSLKMRELGMLTRPLGDVIAFLPPLASTAEELSEMVAIMKQAIHEVTSLED | ||||||
Modified residue | 280 | N6-(pyridoxal phosphate)lysine | ||||
Sequence: K |
Proteomic databases
Structure
Sequence
- Sequence statusComplete
- Length448
- Mass (Da)50,112
- Last updated1996-10-01 v1
- Checksum38F104A93A74AA24
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U51868 EMBL· GenBank· DDBJ | AAB17458.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF008220 EMBL· GenBank· DDBJ | AAC00262.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL009126 EMBL· GenBank· DDBJ | CAB15001.1 EMBL· GenBank· DDBJ | Genomic DNA |