P53355 · DAPK1_HUMAN
- ProteinDeath-associated protein kinase 1
- GeneDAPK1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1430 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]
Cofactor
Activity regulation
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 19-27 | ATP (UniProtKB | ChEBI) | ||||
Sequence: LGSGQFAVV | ||||||
Binding site | 42 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 94-96 | ATP (UniProtKB | ChEBI) | ||||
Sequence: ELV | ||||||
Binding site | 100 | ATP (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 139 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 161 | ATP (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameDeath-associated protein kinase 1
- EC number
- Short namesDAP kinase 1
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionP53355
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Isoform 1
Isoform 2
Keywords
- Cellular component
Disease & Variants
Features
Showing features for mutagenesis, natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 42 | Loss of activity, apoptotic function and of autophosphorylation. | ||||
Sequence: K → A | ||||||
Mutagenesis | 289 | Loss of phosphorylation and significant increase in proapoptotic activity. | ||||
Sequence: S → A | ||||||
Mutagenesis | 289 | Reduction in proapoptotic activity. | ||||
Sequence: S → E | ||||||
Mutagenesis | 308 | Elevated Ca2+-calmodulin binding and Ca2+-calmodulin-independent kinase activity. Increases apoptotic activity. | ||||
Sequence: S → A | ||||||
Mutagenesis | 308 | Reduced Ca2+-calmodulin binding and Ca2+-calmodulin-independent kinase activity. Decreases apoptotic activity. | ||||
Sequence: S → D | ||||||
Mutagenesis | 313 | Minimal effect on activity. | ||||
Sequence: S → A | ||||||
Natural variant | VAR_033235 | 416 | in dbSNP:rs12343465 | |||
Sequence: V → I | ||||||
Natural variant | VAR_040420 | 461 | ||||
Sequence: A → S | ||||||
Natural variant | VAR_040421 | 519 | ||||
Sequence: S → A | ||||||
Natural variant | VAR_040422 | 540 | in dbSNP:rs56327474 | |||
Sequence: C → Y | ||||||
Natural variant | VAR_060693 | 591 | in dbSNP:rs36214022 | |||
Sequence: P → L | ||||||
Natural variant | VAR_060694 | 622 | in dbSNP:rs36215047 | |||
Sequence: I → M | ||||||
Natural variant | VAR_040423 | 941 | ||||
Sequence: M → T | ||||||
Natural variant | VAR_040424 | 977 | ||||
Sequence: R → W | ||||||
Natural variant | VAR_040425 | 978 | ||||
Sequence: K → N | ||||||
Natural variant | VAR_040426 | 993 | ||||
Sequence: Y → C | ||||||
Natural variant | VAR_040427 | 994 | ||||
Sequence: D → E | ||||||
Natural variant | VAR_040428 | 1005 | ||||
Sequence: E → Q | ||||||
Natural variant | VAR_040429 | 1007 | ||||
Sequence: D → Y | ||||||
Natural variant | VAR_040430 | 1008 | ||||
Sequence: L → P | ||||||
Natural variant | VAR_040431 | 1010 | in dbSNP:rs371784492 | |||
Sequence: R → C | ||||||
Natural variant | VAR_040432 | 1018 | ||||
Sequence: T → A | ||||||
Natural variant | VAR_040433 | 1272 | in dbSNP:rs56169226 | |||
Sequence: M → I | ||||||
Natural variant | VAR_040434 | 1346 | in dbSNP:rs1056719 | |||
Sequence: S → N | ||||||
Natural variant | VAR_040435 | 1405 | in dbSNP:rs36220450 | |||
Sequence: G → V |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 1,642 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000085910 | 1-1430 | UniProt | Death-associated protein kinase 1 | |||
Sequence: MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDTKQETLANVSAVNYEFEDEYFSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLLGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILQLLIKRGSRIDVQDKGGSNAVYWAARHGHVDTLKFLSENKCPLDVKDKSGEMALHVAARYGHADVAQLLCSFGSNPNIQDKEEETPLHCAAWHGYYSVAKALCEAGCNVNIKNREGETPLLTASARGYHDIVECLAEHGADLNACDKDGHIALHLAVRRCQMEVIKTLLSQGCFVDYQDRHGNTPLHVACKDGNMPIVVALCEANCNLDISNKYGRTPLHLAANNGILDVVRYLCLMGASVEALTTDGKTAEDLARSEQHEHVAGLLARLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGKTTLVESLKCGLLRSFFRRRRPRLSSTNSSRFPPSPLASKPTVSVSINNLYPGCENVSVRSRSMMFEPGLTKGMLEVFVAPTHHPHCSADDQSTKAIDIQNAYLNGVGDFSVWEFSGNPVYFCCYDYFAANDPTSIHVVVFSLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLQVVLVATHADIMNVPRPAGGEFGYDKDTSLLKEIRNRFGNDLHISNKLFVLDAGASGSKDMKVLRNHLQEIRSQIVSVCPPMTHLCEKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEEDLRRIAQQLHSTGEINIMQSETVQDVLLLDPRWLCTNVLGKLLSVETPRALHHYRGRYTVEDIQRLVPDSDVEELLQILDAMDICARDLSSGTMVDVPALIKTDNLHRSWADEEDEVMVYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWIHQQSTEGDADIRLWVNGCKLANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCLLLDSVCSTIENVMATTLPGLLTVKHYLSPQQLREHHEPVMIYQPRDFFRAQTLKETSLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLTRRKLSRLLDPPDPLGKDWCLLAMNLGLPDLVAKYNTSNGAPKDFLPSPLHALLREWTTYPESTVGTLMSKLRELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSR | |||||||
Modified residue | 289 | UniProt | Phosphoserine; by RPS6KA1 and RPS6KA3 | ||||
Sequence: S | |||||||
Modified residue | 308 | UniProt | Phosphoserine; by autocatalysis | ||||
Sequence: S | |||||||
Modified residue | 319 | UniProt | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 319 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 321 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 324 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 326 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue | 333 | UniProt | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 333 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 366 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue | 734 | UniProt | Phosphoserine; by MAPK1 | ||||
Sequence: S | |||||||
Modified residue | 1115 | UniProt | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Induction
Gene expression databases
Organism-specific databases
Interaction
Subunit
Interacts (via death domain) with MAPK1 and MAPK3 (PubMed:15616583).
Interacts with MAP1B (via N-terminus) (PubMed:18195017).
Interacts with PRKD1 in an oxidative stress-regulated manner (PubMed:17703233).
Interacts with PIN1, PDCD6, BECN1, TSC2 and STX1A (PubMed:12730201, PubMed:16132846, PubMed:18974095, PubMed:19180116, PubMed:21497122).
Interacts (via kinase domain) with DAPK3 (via kinase domain) (PubMed:15367680).
Interacts with GRINB (By similarity).
Interacts (via death domain) with UNC5B (via death domain) (PubMed:15729359).
Interacts with UNC5C (via death domain) (PubMed:27068745).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P53355 | BAG2 O95816 | 3 | EBI-358616, EBI-355275 | |
BINARY | P53355 | BECN1 Q14457 | 4 | EBI-358616, EBI-949378 | |
BINARY | P53355 | CALM3 P0DP25 | 6 | EBI-358616, EBI-397435 | |
BINARY | P53355 | DAPK1 P53355 | 6 | EBI-358616, EBI-358616 | |
BINARY | P53355 | KLHL20 Q9Y2M5 | 15 | EBI-358616, EBI-714379 | |
BINARY | P53355 | LIMA1 Q9UHB6 | 2 | EBI-358616, EBI-351479 | |
BINARY | P53355 | LRRK1 Q38SD2 | 2 | EBI-358616, EBI-1050422 | |
BINARY | P53355 | LRRK2 Q5S007 | 2 | EBI-358616, EBI-5323863 | |
BINARY | P53355 | MAPK3 P27361 | 5 | EBI-358616, EBI-73995 | |
BINARY | P53355 | PDCD6 O75340 | 3 | EBI-358616, EBI-352915 | |
BINARY | P53355 | PKM P14618 | 3 | EBI-358616, EBI-353408 | |
BINARY | P53355 | PKM P14618-1 | 2 | EBI-358616, EBI-4304679 | |
BINARY | P53355 | PPP2R2A P63151 | 3 | EBI-358616, EBI-1048931 | |
BINARY | P53355 | STUB1 Q9UNE7 | 2 | EBI-358616, EBI-357085 |
Protein-protein interaction databases
Chemistry
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 13-275 | Protein kinase | ||||
Sequence: YDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDTKQETLANVSAVNYEFEDEYFSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWI | ||||||
Region | 267-334 | Calmodulin-binding | ||||
Sequence: QDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSD | ||||||
Region | 292-301 | Autoinhibitory domain | ||||
Sequence: NMEKFKKFAA | ||||||
Repeat | 378-407 | ANK 1 | ||||
Sequence: HGTPPLLIAAGCGNIQILQLLIKRGSRIDV | ||||||
Repeat | 411-440 | ANK 2 | ||||
Sequence: GGSNAVYWAARHGHVDTLKFLSENKCPLDV | ||||||
Repeat | 444-473 | ANK 3 | ||||
Sequence: SGEMALHVAARYGHADVAQLLCSFGSNPNI | ||||||
Repeat | 477-506 | ANK 4 | ||||
Sequence: EEETPLHCAAWHGYYSVAKALCEAGCNVNI | ||||||
Repeat | 510-539 | ANK 5 | ||||
Sequence: EGETPLLTASARGYHDIVECLAEHGADLNA | ||||||
Repeat | 543-572 | ANK 6 | ||||
Sequence: DGHIALHLAVRRCQMEVIKTLLSQGCFVDY | ||||||
Repeat | 576-605 | ANK 7 | ||||
Sequence: HGNTPLHVACKDGNMPIVVALCEANCNLDI | ||||||
Repeat | 609-638 | ANK 8 | ||||
Sequence: YGRTPLHLAANNGILDVVRYLCLMGASVEA | ||||||
Domain | 681-955 | Roc | ||||
Sequence: TQNLQPRIKLKLFGHSGSGKTTLVESLKCGLLRSFFRRRRPRLSSTNSSRFPPSPLASKPTVSVSINNLYPGCENVSVRSRSMMFEPGLTKGMLEVFVAPTHHPHCSADDQSTKAIDIQNAYLNGVGDFSVWEFSGNPVYFCCYDYFAANDPTSIHVVVFSLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLQVVLVATHADIMNVPRPAGGEFGYDKDTSLLKEIRNRFGNDLHISNKLFVLDAGASGSKDMKVLRNHLQEIRSQI | ||||||
Repeat | 875-904 | ANK 9 | ||||
Sequence: KLKNPLQVVLVATHADIMNVPRPAGGEFGY | ||||||
Repeat | 1162-1196 | ANK 10 | ||||
Sequence: EGDADIRLWVNGCKLANRGAELLVLLVNHGQGIEV | ||||||
Domain | 1312-1396 | Death | ||||
Sequence: KLSRLLDPPDPLGKDWCLLAMNLGLPDLVAKYNTSNGAPKDFLPSPLHALLREWTTYPESTVGTLMSKLRELGRRDAADFLLKAS |
Domain
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 4 isoforms produced by Alternative splicing.
P53355-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- SynonymsAlpha
- Length1,430
- Mass (Da)160,046
- Last updated2011-01-11 v6
- ChecksumE2C4246E7C78A6D2
P53355-2
- Name2
- Synonymss-DAPK-1
P53355-3
- Name3
- SynonymsBeta
- Differences from canonical
- 1430-1430: R → RRNSHVWNPTV
P53355-4
- Name4
- Differences from canonical
- 805-870: Missing
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F8WCQ3 | F8WCQ3_HUMAN | DAPK1 | 188 |
Sequence caution
Features
Showing features for alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_042053 | 1-446 | in isoform 2 | |||
Sequence: Missing | ||||||
Sequence conflict | 490 | in Ref. 3; BAC87163 | ||||
Sequence: Y → H | ||||||
Alternative sequence | VSP_042054 | 742-783 | in isoform 2 | |||
Sequence: VSVSINNLYPGCENVSVRSRSMMFEPGLTKGMLEVFVAPTHH → GRNLHAGPVSPAGVGFRTLSFQGLGGKGVVFGSLGLYWTLWP | ||||||
Alternative sequence | VSP_042055 | 784-1430 | in isoform 2 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_054478 | 805-870 | in isoform 4 | |||
Sequence: Missing | ||||||
Sequence conflict | 1217 | in Ref. 4; CAH18690 | ||||
Sequence: S → G | ||||||
Alternative sequence | VSP_042056 | 1430 | in isoform 3 | |||
Sequence: R → RRNSHVWNPTV |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X76104 EMBL· GenBank· DDBJ | CAA53712.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AK127855 EMBL· GenBank· DDBJ | BAC87163.1 EMBL· GenBank· DDBJ | mRNA | ||
CR749834 EMBL· GenBank· DDBJ | CAH18690.1 EMBL· GenBank· DDBJ | mRNA | ||
DQ436495 EMBL· GenBank· DDBJ | ABD96827.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL160279 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL161787 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL591852 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH471089 EMBL· GenBank· DDBJ | EAW62727.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC113660 EMBL· GenBank· DDBJ | AAI13661.1 EMBL· GenBank· DDBJ | mRNA | ||
BC143733 EMBL· GenBank· DDBJ | AAI43734.1 EMBL· GenBank· DDBJ | mRNA | ||
BC143759 EMBL· GenBank· DDBJ | AAI43760.1 EMBL· GenBank· DDBJ | mRNA | ||
BT006935 EMBL· GenBank· DDBJ | AAP35581.1 EMBL· GenBank· DDBJ | mRNA | Sequence problems. |