P53185 · VIR_YEAST
- ProteinProtein virilizer
- GeneVIR1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids909 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Component of the MIS complex, a complex that mediates N6-methyladenosine (m6A) methylation of meiotic mRNAs and is required for initiation of meiosis, progression through the meiotic divisions and sporulation (PubMed:36930734).
In the complex, performs a scaffolding role stabilizing the other complex members (PubMed:36930734).
In the complex, performs a scaffolding role stabilizing the other complex members (PubMed:36930734).
Miscellaneous
Present with 486 molecules/cell in log phase SD medium.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | mediator complex | |
Cellular Component | nucleolus | |
Cellular Component | RNA N6-methyladenosine methyltransferase complex | |
Cellular Component | transcription regulator complex | |
Biological Process | meiotic cell cycle | |
Biological Process | positive regulation of transcription by RNA polymerase II |
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein virilizer
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP53185
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Severely decreases N6-methyladenosine (m6A) levels following meiotic induction (PubMed:36930734).
Abnormal cell cycle arrest in meiotic interphase before premeiotic DNA replication (PubMed:36930734).
Increases the rate of degradation of KAR4, MUM2 and IME4 protein (PubMed:36930734).
Increases RME1 protein levels, and decreases IME1 transcript levels (PubMed:36930734).
Decreases RNA level of genes involved in translation and ribosome biogenesis, and early meiotic genes (PubMed:36930734).
Partially suppressed by disruption of RME1 (PubMed:36930734).
Abnormal cell cycle arrest in meiotic interphase before premeiotic DNA replication (PubMed:36930734).
Increases the rate of degradation of KAR4, MUM2 and IME4 protein (PubMed:36930734).
Increases RME1 protein levels, and decreases IME1 transcript levels (PubMed:36930734).
Decreases RNA level of genes involved in translation and ribosome biogenesis, and early meiotic genes (PubMed:36930734).
Partially suppressed by disruption of RME1 (PubMed:36930734).
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 27 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000096613 | 1-909 | Protein virilizer | |||
Sequence: MQKCAGHAPLVTAASRVSQDTVDALLQAILKAYHKLASIDSHINDPVEIAFKLINSFKYLPISGSSVKDFESELRELDVFSPLLQSAVTAANNSNIIWDLIAVLFAYISIHKQLHPLILHNLNIWKDFMADNDEETATTTDGDSMNFGVLSLLSIVQNFEEITPNLFEFLKLGLRSTLLKIWVAQWQRYDPSATNLINGDEKISSWITKDYQVDFFIITSLASTSSLEVLPSHYFVYKISKRISHFPNLIDPKLYRSAISTIMENGISDNGGGENSSDKIDPTDLSFHLQVLMEVIDHPELNYLQENRLILLLDIALNYLILVPTHCLHSNFGELGSTQSLASTLNIIQFLLSKFLINMGSISQLINQYNRKCITTNNINNNNINNNGVINGSTNTTSTTTTTITNNNNNSNNSSISNNNRKIDWTQSYQTRYQIPYWFEDSILPPIPPISKSLFTFDKNLDHESDSIMIVNDVLRCLNLTILLISKLLRDYDDLKINPLIQSSDDHSNEDNHVIIEQYMQLYLVPLFTSLLLAQQLKDRGQERDEGHKEKEENINLIGSSSVKKLFSQLIFFSSLKLCENLVIKEKNLALYHLIKFATKVSLDDLILQKISINLLNHLFFHQIRDGSDDDNLIKKLCLKNQLSFQALKDYITLWNDGSEVYNAFYKELFYEEQPKIKPIKLTTSDLLKLFPEDVQFVISTPPNTITSASTSDNCTSSQSAAQKNIENFTTLSKYDVYSSTSFIPSTSKNTNTNVSKQQQQPQNSTPCSSNRFLFNKSSLISQESNGSNNNSGTQGPGSMNESYSLDNSFNTTNTNMTRQPTTLTRATDAMTTAPTTPIPYKNTSGSSNNNLWIESPMTNFKGSTISKSTNKSKMVNTGKNYILGGHNKVKNNSRAQSIHIDDFENENN | ||||||
Modified residue | 856 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 898 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Component of the MIS (mRNA N6-methyladenosine (m6A) methylation) complex, at least composed of IME4, KAR4, MUM2, SLZ1, and VIR1 (PubMed:36930734).
Interacts with KAR4 (PubMed:36930734).
Interacts with SLZ1 (PubMed:36930734).
Interacts with MUM2 (PubMed:36930734).
Interacts with IME4 (PubMed:36930734).
Interacts with KAR4 (PubMed:36930734).
Interacts with SLZ1 (PubMed:36930734).
Interacts with MUM2 (PubMed:36930734).
Interacts with IME4 (PubMed:36930734).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P53185 | MUM2 P38236 | 3 | EBI-11537, EBI-11619 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 746-824 | Disordered | ||||
Sequence: STSKNTNTNVSKQQQQPQNSTPCSSNRFLFNKSSLISQESNGSNNNSGTQGPGSMNESYSLDNSFNTTNTNMTRQPTTL |
Sequence similarities
Belongs to the vir family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length909
- Mass (Da)102,371
- Last updated1996-10-01 v1
- Checksum5B1849B0F983175B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z72558 EMBL· GenBank· DDBJ | CAA96738.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z72557 EMBL· GenBank· DDBJ | CAA96737.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006941 EMBL· GenBank· DDBJ | DAA08064.1 EMBL· GenBank· DDBJ | Genomic DNA |