P53112 · PEX14_YEAST
- ProteinPeroxisomal membrane protein PEX14
- GenePEX14
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids341 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 or PEX21 receptors (PubMed:20154681, PubMed:9094717).
The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm (PubMed:20154681).
Mechanistically, PEX5 (or PEX21) receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix (PubMed:20154681).
The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm (PubMed:20154681).
Mechanistically, PEX5 (or PEX21) receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix (PubMed:20154681).
Miscellaneous
Present with 2570 molecules/cell in log phase SD medium.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | peroxisomal importomer complex | |
Cellular Component | peroxisomal membrane | |
Molecular Function | protein transmembrane transporter activity | |
Molecular Function | protein-macromolecule adaptor activity | |
Molecular Function | signaling receptor binding | |
Biological Process | protein import into peroxisome matrix, docking |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended namePeroxisomal membrane protein PEX14
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP53112
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Peroxisome membrane ; Peripheral membrane protein
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 87-90 | Loss of interaction with SH3 domain of PEX13. No effect on membrane association. | ||||
Sequence: PTLP → ATLA |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 1 variant from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylserine | ||||
Sequence: S | ||||||
Chain | PRO_0000058329 | 2-341 | Peroxisomal membrane protein PEX14 | |||
Sequence: SDVVSKDRKALFDSAVSFLKDESIKDAPLLKKIEFLKSKGLTEKEIEIAMKEPKKDGIVGDEVSKKIGSTENRASQDMYLYEAMPPTLPHRDWKDYFVMATATAGLLYGAYEVTRRYVIPNILPEAKSKLEGDKKEIDDQFSKIDTVLNAIEAEQAEFRKKESETLKELSDTIAELKQALVQTTRSREKIEDEFRIVKLEVVNMQNTIDKFVSDNDGMQELNNIQKEMESLKSLMNNRMESGNAQDNRLFSISPNGIPGIDTIPSASEILAKMGMQEESDKEKENGSDANKDDNAVPAWKKAREQTIDSNASIPEWQKNTAANEISVPDWQNGQVEDSIP | ||||||
Modified residue | 313 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Interacts with PEX13 (via SH3 domain); forming the PEX13-PEX14 docking complex (PubMed:10087260, PubMed:12453410, PubMed:20154681).
Interacts with PEX5 (via WxxxF/Y motifs) (PubMed:20154681, PubMed:9094717).
Interacts with PEX7 (PubMed:9094717).
Interacts with PEX9 (PubMed:27678487).
Interacts with PEX5 (via WxxxF/Y motifs) (PubMed:20154681, PubMed:9094717).
Interacts with PEX7 (PubMed:9094717).
Interacts with PEX9 (PubMed:27678487).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P53112 | FRQ1 Q06389 | 3 | EBI-13212, EBI-11946 | |
BINARY | P53112 | PEX13 P80667 | 18 | EBI-13212, EBI-13206 | |
BINARY | P53112 | PEX17 P40155 | 15 | EBI-13212, EBI-13218 | |
BINARY | P53112 | PEX2 P32800 | 11 | EBI-13212, EBI-13160 | |
BINARY | P53112 | PEX5 P35056 | 9 | EBI-13212, EBI-13170 | |
BINARY | P53112 | PEX6 P33760 | 6 | EBI-13212, EBI-13178 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for motif, compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 86-94 | SH3-binding | ||||
Sequence: PPTLPHRDW | ||||||
Compositional bias | 276-300 | Basic and acidic residues | ||||
Sequence: MQEESDKEKENGSDANKDDNAVPAW | ||||||
Region | 276-341 | Disordered | ||||
Sequence: MQEESDKEKENGSDANKDDNAVPAWKKAREQTIDSNASIPEWQKNTAANEISVPDWQNGQVEDSIP | ||||||
Compositional bias | 306-341 | Polar residues | ||||
Sequence: QTIDSNASIPEWQKNTAANEISVPDWQNGQVEDSIP |
Sequence similarities
Belongs to the peroxin-14 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length341
- Mass (Da)38,420
- Last updated1996-10-01 v1
- ChecksumB714D7DA045E2DCB
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 276-300 | Basic and acidic residues | ||||
Sequence: MQEESDKEKENGSDANKDDNAVPAW | ||||||
Compositional bias | 306-341 | Polar residues | ||||
Sequence: QTIDSNASIPEWQKNTAANEISVPDWQNGQVEDSIP |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z48618 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
Z72675 EMBL· GenBank· DDBJ | CAA96864.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY558503 EMBL· GenBank· DDBJ | AAS56829.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006941 EMBL· GenBank· DDBJ | DAA07959.1 EMBL· GenBank· DDBJ | Genomic DNA |