P53068 · APC10_YEAST

Function

function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication. DOC1 is required, together with the coactivators CDH1 and CDC20, for recognition and binding of the substrates.

Miscellaneous

Present with 1364 molecules/cell in log phase SD medium.

Pathway

Protein modification; protein ubiquitination.

GO annotations

AspectTerm
Cellular Componentanaphase-promoting complex
Cellular Componentmitochondrion
Molecular Functionenzyme regulator activity
Biological Processanaphase-promoting complex-dependent catabolic process
Biological Processcell division
Biological Processchromatin organization
Biological Processprotein K11-linked ubiquitination
Biological Processprotein ubiquitination
Biological Processregulation of meiotic cell cycle
Biological Processregulation of mitotic cell cycle

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Anaphase-promoting complex subunit DOC1
  • Alternative names
    • Destruction of cyclin B protein 1

Gene names

    • Name
      DOC1
    • Synonyms
      APC10
    • ORF names
      HRC283
    • Ordered locus names
      YGL240W

Organism names

Accessions

  • Primary accession
    P53068
  • Secondary accessions
    • D6VV95

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis94In DOC1-1; G2/M cell cycle arrest at 37 degrees Celsius.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001740161-250Anaphase-promoting complex subunit DOC1

Proteomic databases

PTM databases

Interaction

Subunit

The APC/C is composed of at least 13 subunits that stay tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5, APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P53068APC1 P5388610EBI-2603, EBI-29017
BINARY P53068CDC16 P0979814EBI-2603, EBI-4208
BINARY P53068CDC27 P380425EBI-2603, EBI-4249

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain60-246DOC

Sequence similarities

Belongs to the APC10 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    250
  • Mass (Da)
    28,777
  • Last updated
    2004-11-09 v2
  • Checksum
    F3C58B1BB986442D
MDPIGINKVLDHLAPSELIKPVKSCHNKPSVLVLDDRIVDAATKDLYVNGFQEEIQYQNPTPENLQHMFHQGIEILDSARMINVTHLALWKPSSFKLGNPVDFALDDNYDTFWQSDGGQPHQLDIMFSKRMDICVMAIFFSMIADESYAPSLVKVYAGHSPSDARFYKMLEVRNVNGWVALRFLDNREDDQLLKCQFIRLLFPVNHENGKDTHLRGIRLYVPSNEPHQDTHEWAQTLPETNNVFQDAILR

Sequence caution

The sequence CAA89016.1 differs from that shown. Reason: Erroneous initiation
The sequence CAA96959.1 differs from that shown. Reason: Erroneous initiation

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z49149
EMBL· GenBank· DDBJ
CAA89016.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
Z72762
EMBL· GenBank· DDBJ
CAA96959.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
Z72761
EMBL· GenBank· DDBJ
CAA96958.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006941
EMBL· GenBank· DDBJ
DAA07879.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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