P53043 · PPT1_YEAST

Function

function

Protein phosphatase that specifically binds to and dephosphorylates the molecular chaperone Hsp90 (HSC82 and HSP82). Dephosphorylation positively regulates the Hsp90 chaperone machinery.

Miscellaneous

Present with 6990 molecules/cell in log phase SD medium.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 manganese ions per subunit.

Activity regulation

Stimulated by arachidonic acid and other unsaturated fatty acids, and by arachidoyl coenzyme A.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
34 mMp-nitrophenylphosphate

pH Dependence

Optimum pH is 7.8.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site249Mn2+ 1 (UniProtKB | ChEBI)
Binding site251Mn2+ 1 (UniProtKB | ChEBI)
Binding site278Mn2+ 1 (UniProtKB | ChEBI)
Binding site278Mn2+ 2 (UniProtKB | ChEBI)
Binding site310Mn2+ 2 (UniProtKB | ChEBI)
Active site311Proton donor
Binding site359Mn2+ 2 (UniProtKB | ChEBI)
Binding site434Mn2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentnucleus
Molecular Functionmetal ion binding
Molecular Functionmyosin phosphatase activity
Molecular Functionprotein serine/threonine phosphatase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Serine/threonine-protein phosphatase T
  • EC number
  • Short names
    PPT

Gene names

    • Name
      PPT1
    • ORF names
      G6347
    • Ordered locus names
      YGR123C

Organism names

Accessions

  • Primary accession
    P53043
  • Secondary accessions
    • D6VUQ5

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis311Loss of phosphatase activity.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 6 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000588981-513Serine/threonine-protein phosphatase T

Proteomic databases

PTM databases

Expression

Induction

Expression peaks in early log phase and decreases dramatically during the stationary phase (at protein level).

Interaction

Subunit

Interacts (via TPR repeats) with HSP82 (via C-terminal MEEVD pentapeptide).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P53043HSC82 P151083EBI-13796, EBI-8666
BINARY P53043HSP82 P028299EBI-13796, EBI-8659

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for repeat, region.

TypeIDPosition(s)Description
Repeat12-45TPR 1
Repeat46-79TPR 2
Repeat80-113TPR 3
Region188-513Catalytic

Domain

The TPR repeats mediate protein-protein interactions with substrate proteins, but also autoinhibit PPT1 phosphatase activity.

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    513
  • Mass (Da)
    57,995
  • Last updated
    1996-10-01 v1
  • Checksum
    6966DA22340A5793
MSTPTAADRAKALERKNEGNVFVKEKHFLKAIEKYTEAIDLDSTQSIYFSNRAFAHFKVDNFQSALNDCDEAIKLDPKNIKAYHRRALSCMALLEFKKARKDLNVLLKAKPNDPAATKALLTCDRFIREERFRKAIGGAENEAKISLCQTLNLSSFDANADLANYEGPKLEFEQLYDDKNAFKGAKIKNMSQEFISKMVNDLFLKGKYLPKKYVAAIISHADTLFRQEPSMVELENNSTPDVKISVCGDTHGQFYDVLNLFRKFGKVGPKHTYLFNGDFVDRGSWSCEVALLFYCLKILHPNNFFLNRGNHESDNMNKIYGFEDECKYKYSQRIFNMFAQSFESLPLATLINNDYLVMHGGLPSDPSATLSDFKNIDRFAQPPRDGAFMELLWADPQEANGMGPSQRGLGHAFGPDITDRFLRNNKLRKIFRSHELRMGGVQFEQKGKLMTVFSAPNYCDSQGNLGGVIHVVPGHGILQAGRNDDQNLIIETFEAVEHPDIKPMAYSNGGFGL

Sequence caution

The sequence CAA61596.1 differs from that shown. Reason: Frameshift

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X89417
EMBL· GenBank· DDBJ
CAA61596.1
EMBL· GenBank· DDBJ
Genomic DNA Frameshift
X83099
EMBL· GenBank· DDBJ
CAA58158.1
EMBL· GenBank· DDBJ
Genomic DNA
Z72908
EMBL· GenBank· DDBJ
CAA97134.1
EMBL· GenBank· DDBJ
Genomic DNA
AY558095
EMBL· GenBank· DDBJ
AAS56421.1
EMBL· GenBank· DDBJ
Genomic DNA
S39959
EMBL· GenBank· DDBJ
AAB22462.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006941
EMBL· GenBank· DDBJ
DAA08216.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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