P53039 · YIP1_YEAST
- ProteinProtein transport protein YIP1
- GeneYIP1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids248 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Required for fusion of ER-derived vesicles with the Golgi during ER-to-Golgi protein transport, probably by mediating correct membrane localization of YPT1.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | COPII-coated ER to Golgi transport vesicle | |
Cellular Component | endoplasmic reticulum | |
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | Golgi membrane | |
Cellular Component | trans-Golgi network | |
Biological Process | endoplasmic reticulum to Golgi vesicle-mediated transport | |
Biological Process | protein transport | |
Biological Process | vesicle fusion with Golgi apparatus | |
Biological Process | vesicle-mediated transport |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameProtein transport protein YIP1
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP53039
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Golgi apparatus membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-110 | Cytoplasmic | ||||
Sequence: MSFYNTSNNANNGGGFYQPSAQFAVPQGSMSFQNTVGSSNTGNDNNLGVAPDPLPVGILHALSTKGYPHEPPLLEEIGINFDHIITKTKMVLIPIRFGSGVPQEILNDSD | ||||||
Transmembrane | 111-131 | Helical | ||||
Sequence: LAGPLIFFLLFGLFLLMAGKV | ||||||
Topological domain | 132 | Lumenal | ||||
Sequence: H | ||||||
Transmembrane | 133-153 | Helical | ||||
Sequence: FGYIYGVALFGTISLHNLSKL | ||||||
Topological domain | 154-172 | Cytoplasmic | ||||
Sequence: MSNNDTSTQTNLQFFNTAS | ||||||
Transmembrane | 173-193 | Helical | ||||
Sequence: ILGYCFLPLCFLSLLGIFHGL | ||||||
Topological domain | 194-196 | Lumenal | ||||
Sequence: NNT | ||||||
Transmembrane | 197-217 | Helical | ||||
Sequence: TGYVVSVLFVIWSTWTSSGFL | ||||||
Topological domain | 218-227 | Cytoplasmic | ||||
Sequence: NSLLQLQNAR | ||||||
Transmembrane | 228-248 | Helical | ||||
Sequence: LLIAYPLLIFYSVFALMVIFV |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 70 | In YIP1-41; lethal; abolishes binding to YIF1, YPT1 and YPT31. In YIP1-40; temperature sensitive; reduces binding to YPT31, and inhibits ER vesicle budding; when associated with A-130. | ||||
Sequence: E → G | ||||||
Mutagenesis | 70 | In YIP1-4; temperature sensitive; reduces binding to YIF1, YPT1 and YPT31. In YIP1-44; lethal; when associated with E-130. | ||||
Sequence: E → K | ||||||
Mutagenesis | 70 | In YIP1-42; temperature sensitive; abolishes binding to YPT1 and YPT31, and inhibits ER vesicle budding. | ||||
Sequence: E → V | ||||||
Mutagenesis | 76 | In YIP1-6; lethal; reduces binding to YIF1. | ||||
Sequence: E → K | ||||||
Mutagenesis | 81 | In YIP1-9; lethal. | ||||
Sequence: F → Y | ||||||
Mutagenesis | 114 | In YIP1-1; blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius; when associated with E-129. | ||||
Sequence: P → L | ||||||
Mutagenesis | 129 | In YIP1-1; blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius; when associated with L-114. | ||||
Sequence: G → E | ||||||
Mutagenesis | 130 | In YIP1-40; temperature sensitive; reduces binding to YPT31, and inhibits ER vesicle budding; when associated with G-70. | ||||
Sequence: K → A | ||||||
Mutagenesis | 130 | In YIP1-44; lethal; when associated with K-70. | ||||
Sequence: K → E | ||||||
Mutagenesis | 153-155 | In YIP1-12; abolishes binding to YPT1 and YPT31. | ||||
Sequence: LMS → GGG | ||||||
Mutagenesis | 175 | In YIP1-2; abolishes binding to YPT1 and YPT31, reduces binding to YIF1, blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius. | ||||
Sequence: G → E | ||||||
Mutagenesis | 177 | In YIP1-18; abolishes binding to YIF1, YPT1 and YPT31. | ||||
Sequence: C → S | ||||||
Mutagenesis | 180 | In YIP1-19; lethal; abolishes binding to YPT1 and YPT31, and reduces binding to YIF1. | ||||
Sequence: P → G |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 4 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000066269 | 1-248 | Protein transport protein YIP1 | |||
Sequence: MSFYNTSNNANNGGGFYQPSAQFAVPQGSMSFQNTVGSSNTGNDNNLGVAPDPLPVGILHALSTKGYPHEPPLLEEIGINFDHIITKTKMVLIPIRFGSGVPQEILNDSDLAGPLIFFLLFGLFLLMAGKVHFGYIYGVALFGTISLHNLSKLMSNNDTSTQTNLQFFNTASILGYCFLPLCFLSLLGIFHGLNNTTGYVVSVLFVIWSTWTSSGFLNSLLQLQNARLLIAYPLLIFYSVFALMVIFV |
Proteomic databases
Interaction
Subunit
Component of the YIP1-YIF1 complex, composed of at least YIF1, YIP1 and YOS1. The complex interacts with the ER to Golgi SNAREs BOS1 and SEC22. Interacts with the prenylated form of the Rab GTPases YPT1 and YPT31. Interacts with the YIP1 family members YIP4 and YIP5. Interacts with SNX3, YIP3 and YOP1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P53039 | ATG20 Q07528 | 2 | EBI-25295, EBI-36894 | |
BINARY | P53039 | VPS17 P32913 | 2 | EBI-25295, EBI-20366 | |
BINARY | P53039 | YIF1 P53845 | 5 | EBI-25295, EBI-28230 | |
BINARY | P53039 | YPT35 P38815 | 3 | EBI-25295, EBI-24665 |
Protein-protein interaction databases
Miscellaneous
Structure
Sequence
- Sequence statusComplete
- Length248
- Mass (Da)27,080
- Last updated1996-10-01 v1
- Checksum64372B4823A0BC5E
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X97342 EMBL· GenBank· DDBJ | CAA66031.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z72957 EMBL· GenBank· DDBJ | CAA97198.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006941 EMBL· GenBank· DDBJ | DAA08267.1 EMBL· GenBank· DDBJ | Genomic DNA |