P52946 · PDX1_MOUSE

  • Protein
    Pancreas/duodenum homeobox protein 1
  • Gene
    Pdx1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'.

Features

Showing features for dna binding.

128420406080100120140160180200220240260280
TypeIDPosition(s)Description
DNA binding147-206Homeobox

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytosol
Cellular Componentnuclear speck
Cellular Componentnucleoplasm
Cellular Componentnucleus
Molecular Functionchromatin binding
Molecular FunctionDNA-binding transcription activator activity, RNA polymerase II-specific
Molecular FunctionDNA-binding transcription factor activity
Molecular FunctionDNA-binding transcription factor activity, RNA polymerase II-specific
Molecular Functionpromoter-specific chromatin binding
Molecular Functionprotein-containing complex binding
Molecular FunctionRNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Functionsequence-specific DNA binding
Biological Processanimal organ regeneration
Biological Processcell differentiation
Biological Processcell population proliferation
Biological Processdigestive tract development
Biological ProcessDNA-templated transcription
Biological Processendocrine pancreas development
Biological Processenteroendocrine cell differentiation
Biological Processepithelial cell proliferation
Biological Processexocrine pancreas development
Biological Processglucose homeostasis
Biological Processglucose mediated signaling pathway
Biological Processglucose metabolic process
Biological Processinsulin secretion
Biological Processintrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
Biological Processliver development
Biological Processmorphogenesis of embryonic epithelium
Biological Processnegative regulation of cell population proliferation
Biological Processnegative regulation of DNA-templated transcription
Biological Processnegative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
Biological Processnegative regulation of epithelial cell proliferation
Biological Processnegative regulation of transcription by RNA polymerase II
Biological Processnegative regulation of type B pancreatic cell apoptotic process
Biological Processpancreas development
Biological Processpositive regulation of cell population proliferation
Biological Processpositive regulation of DNA-templated transcription
Biological Processpositive regulation of insulin secretion
Biological Processpositive regulation of insulin secretion involved in cellular response to glucose stimulus
Biological Processpositive regulation of transcription by RNA polymerase II
Biological Processpositive regulation of type B pancreatic cell proliferation
Biological Processregulation of cell population proliferation
Biological Processregulation of gene expression
Biological Processregulation of transcription by RNA polymerase II
Biological Processresponse to alkaloid
Biological Processresponse to chlorate
Biological Processresponse to cytokine
Biological Processresponse to fatty acid
Biological Processresponse to glucocorticoid
Biological Processresponse to glucose
Biological Processresponse to iron(II) ion
Biological Processresponse to L-leucine
Biological Processresponse to nicotine
Biological Processresponse to vitamin
Biological Processresponse to xenobiotic stimulus
Biological Processsmoothened signaling pathway
Biological Processstem cell differentiation
Biological Processtranscription by RNA polymerase II
Biological Processtransdifferentiation
Biological Processtype B pancreatic cell apoptotic process
Biological Processtype B pancreatic cell differentiation
Biological Processtype B pancreatic cell proliferation

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Pancreas/duodenum homeobox protein 1
  • Alternative names
    • Insulin promoter factor 1 (IPF-1)
    • Islet/duodenum homeobox 1 (IDX-1)
    • Somatostatin-transactivating factor 1 (STF-1)

Gene names

    • Name
      Pdx1
    • Synonyms
      Ipf1

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P52946
  • Secondary accessions
    • Q3ZB03

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis152Displays a more cytosolic distribution.
Mutagenesis269Reduced phosphorylation by HIPK2.
Mutagenesis269Abnormal subnuclear localization upon glucose accumulation.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 21 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000491491-284Pancreas/duodenum homeobox protein 1
Modified residue152Phosphothreonine; by PASK
Modified residue269Phosphoserine; by HIPK2

Post-translational modification

Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration. Phosphorylated by HIPK2 on Ser-269 upon glucose accumulation. This phosphorylation mediates subnuclear localization shifting. Phosphorylation by PASK may lead to translocation into the cytosol.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).

Induction

Expression is repressed by FOXO1 in pancreatic beta-cells.

Developmental stage

At 8.5 dpc, detected in the gut epithelium from which the pancreatic buds are formed. Transient expression in pancreatic ducts, endocrine and acinar cells. Down-regulated around 10.5 dpc when expression becomes restricted to differentiated beta-cells.

Gene expression databases

Interaction

Subunit

Interacts with the basic helix-loop-helix domains of TCF3(E47) and NEUROD1 and with HMG-I(Y). Interacts with the methyltransferase SETD7 (By similarity).
Interacts with SPOP. Part of a PDX1:PBX1b:MEIS2b complex

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P52946Rb1 P134052EBI-7128945, EBI-971782
BINARY P52946Spop Q6ZWS85EBI-7128945, EBI-7128920

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, compositional bias, motif.

TypeIDPosition(s)Description
Region13-73Transactivation domain
Region30-116Disordered
Compositional bias37-51Pro residues
Motif119-124Antp-type hexapeptide
Motif198-204Nuclear localization signal
Region202-284Disordered

Domain

The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y).

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    284
  • Mass (Da)
    30,999
  • Last updated
    1996-10-01 v1
  • Checksum
    A57D04569D14E3C4
MNSEEQYYAATQLYKDPCAFQRGPVPEFSANPPACLYMGRQPPPPPPPQFTSSLGSLEQGSPPDISPYEVPPLASDDPAGAHLHHHLPAQLGLAHPPPGPFPNGTEPGGLEEPNRVQLPFPWMKSTKAHAWKGQWAGGAYTAEPEENKRTRTAYTRAQLLELEKEFLFNKYISRPRRVELAVMLNLTERHIKIWFQNRRMKWKKEEDKKRSSGTPSGGGGGEEPEQDCAVTSGEELLAVPPLPPPGGAVPPGVPAAVREGLLPSGLSVSPQPSSIAPLRPQEPR

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias37-51Pro residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X74342
EMBL· GenBank· DDBJ
CAA52389.1
EMBL· GenBank· DDBJ
mRNA
AK020261
EMBL· GenBank· DDBJ
BAB32045.1
EMBL· GenBank· DDBJ
mRNA
BC103572
EMBL· GenBank· DDBJ
AAI03573.1
EMBL· GenBank· DDBJ
mRNA
BC103581
EMBL· GenBank· DDBJ
AAI03582.1
EMBL· GenBank· DDBJ
mRNA
BC103582
EMBL· GenBank· DDBJ
AAI03583.1
EMBL· GenBank· DDBJ
mRNA
BC105642
EMBL· GenBank· DDBJ
AAI05643.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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