P52920 · NBP35_YEAST

Function

function

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Required for biogenesis and export of both ribosomal subunits, which may reflect a role in assembly of the Fe/S clusters in RLI1, a protein which performs rRNA processing and ribosome export.

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )
Note: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NBP35 and two labile, bridging clusters between subunits of the NBP35-CFD1 heterotetramer.

Features

Showing features for binding site.

132850100150200250300
TypeIDPosition(s)Description
Binding site27[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site41[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site44[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site50[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site80-87ATP (UniProtKB | ChEBI)
Binding site253[4Fe-4S] cluster 2 (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site256[4Fe-4S] cluster 2 (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentcytosolic [4Fe-4S] assembly scaffold complex
Cellular Componentiron-sulfur cluster assembly complex
Cellular Componentnucleus
Molecular Function4 iron, 4 sulfur cluster binding
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionATP-dependent FeS chaperone activity
Molecular Functioniron ion binding
Molecular Functioniron-sulfur cluster binding
Biological Processiron-sulfur cluster assembly
Biological ProcesstRNA wobble uridine modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytosolic Fe-S cluster assembly factor NBP35
  • Alternative names
    • Nucleotide-binding protein 35

Gene names

    • Name
      NBP35
    • Ordered locus names
      YGL091C

Organism names

Accessions

  • Primary accession
    P52920
  • Secondary accessions
    • D6VU54

Proteomes

Organism-specific databases

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis27Supports growth, albeit at a lower rate.
Mutagenesis41Loss of function.
Mutagenesis44Loss of function.
Mutagenesis50Loss of function.
Mutagenesis86Lethal.
Mutagenesis234Does not impair function.
Mutagenesis253Loss fo function and disrupts heterotetramer formation.
Mutagenesis256Loss of function and disrupts heterotetramer formation.
Mutagenesis295Does not impair function.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001849471-328Cytosolic Fe-S cluster assembly factor NBP35

Proteomic databases

PTM databases

Interaction

Subunit

Heterotetramer of 2 NBP35 and 2 CFD1 chains.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntAct
BINARY P52920CFD1 P4055811EBI-11880, EBI-24924

Complex viewer

View interactors in UniProtKB
View CPX-385 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    328
  • Mass (Da)
    35,253
  • Last updated
    1996-10-01 v1
  • Checksum
    4B2EADFD0555BCAA
MTEILPHVNDEVLPAEYELNQPEPEHCPGPESDMAGKSDACGGCANKEICESLPKGPDPDIPLITDNLSGIEHKILVLSGKGGVGKSTFAAMLSWALSADEDLQVGAMDLDICGPSLPHMLGCIKETVHESNSGWTPVYVTDNLATMSIQYMLPEDDSAIIWRGSKKNLLIKKFLKDVDWDKLDYLVIDTPPGTSDEHISINKYMRESGIDGALVVTTPQEVALLDVRKEIDFCKKAGINILGLVENMSGFVCPNCKGESQIFKATTGGGEALCKELGIKFLGSVPLDPRIGKSCDMGESFLDNYPDSPASSAVLNVVEALRDAVGDV

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict58in Ref. 1; CAA64779

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X95533
EMBL· GenBank· DDBJ
CAA64779.1
EMBL· GenBank· DDBJ
Genomic DNA
Z72613
EMBL· GenBank· DDBJ
CAA96797.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006941
EMBL· GenBank· DDBJ
DAA08015.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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