P52920 · NBP35_YEAST
- ProteinCytosolic Fe-S cluster assembly factor NBP35
- GeneNBP35
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids328 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Required for biogenesis and export of both ribosomal subunits, which may reflect a role in assembly of the Fe/S clusters in RLI1, a protein which performs rRNA processing and ribosome export.
Cofactor
Note: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NBP35 and two labile, bridging clusters between subunits of the NBP35-CFD1 heterotetramer.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 27 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 41 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 44 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 50 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 80-87 | ATP (UniProtKB | ChEBI) | |||
Binding site | 253 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
Binding site | 256 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | cytosolic [4Fe-4S] assembly scaffold complex | |
Cellular Component | iron-sulfur cluster assembly complex | |
Cellular Component | nucleus | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | ATP-dependent FeS chaperone activity | |
Molecular Function | iron ion binding | |
Molecular Function | iron-sulfur cluster binding | |
Biological Process | iron-sulfur cluster assembly | |
Biological Process | tRNA wobble uridine modification |
Keywords
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytosolic Fe-S cluster assembly factor NBP35
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP52920
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 27 | Supports growth, albeit at a lower rate. | |||
Mutagenesis | 41 | Loss of function. | |||
Mutagenesis | 44 | Loss of function. | |||
Mutagenesis | 50 | Loss of function. | |||
Mutagenesis | 86 | Lethal. | |||
Mutagenesis | 234 | Does not impair function. | |||
Mutagenesis | 253 | Loss fo function and disrupts heterotetramer formation. | |||
Mutagenesis | 256 | Loss of function and disrupts heterotetramer formation. | |||
Mutagenesis | 295 | Does not impair function. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000184947 | 1-328 | Cytosolic Fe-S cluster assembly factor NBP35 | ||
Proteomic databases
PTM databases
Interaction
Subunit
Heterotetramer of 2 NBP35 and 2 CFD1 chains.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | P52920 | CFD1 P40558 | 11 | EBI-11880, EBI-24924 |
Complex viewer
Protein-protein interaction databases
Miscellaneous
Structure
Sequence
- Sequence statusComplete
- Length328
- Mass (Da)35,253
- Last updated1996-10-01 v1
- Checksum4B2EADFD0555BCAA
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 58 | in Ref. 1; CAA64779 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X95533 EMBL· GenBank· DDBJ | CAA64779.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z72613 EMBL· GenBank· DDBJ | CAA96797.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006941 EMBL· GenBank· DDBJ | DAA08015.1 EMBL· GenBank· DDBJ | Genomic DNA |