P52839 · SOT12_ARATH
- ProteinCytosolic sulfotransferase 12
- GeneSOT12
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids326 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the stereospecific sulfate conjugation of 24-epibrassinosteroids. Preferred substrates are 24-epicathasterone and 6-deoxo-24-epicathasterone. Low activity with 22-deoxy-24-epiteasterone. No activity with 24-epimers catasterone and brassinolide. Sulfonates salicylic acid. May be involved in detoxification. Enhances plant response to pathogen infection and contributes to long distance signaling in systemic acquired resistance (SAR).
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
6.9 μM | 24-epicathasterone | |||||
1.9 μM | 6-deoxo-24-epicathasterone | |||||
3 μM | 17-beta-estradiol | |||||
1.1 μM | dehydroepiandrosterone | |||||
13 μM | pregnenolone | |||||
0.44 mM | salicylic acid | |||||
3 μM | 3'-phospho-5'-adenylyl sulfate |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
57 pmol/sec/mg | with 24-epicathasterone as substrate | ||||
4.4 pmol/sec/mg | with 24-epicathasterone as substrate | ||||
1.6 pmol/sec/mg | with 17-beta-estradiol as substrate | ||||
1.3 pmol/sec/mg | with dehydroepiandrosterone as substrate | ||||
5.9 pmol/sec/mg | with pregnenolone as substrate | ||||
2.67 pmol/sec/mg | with salicylic acid as substrate |
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 75-80 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | |||
Active site | 140 | Proton acceptor | |||
Binding site | 162 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | |||
Binding site | 170 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | |||
Binding site | 228 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | |||
Binding site | 290-292 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Golgi apparatus | |
Molecular Function | brassinosteroid sulfotransferase activity | |
Molecular Function | flavonoid sulfotransferase activity | |
Molecular Function | sulfotransferase activity | |
Biological Process | brassinosteroid metabolic process | |
Biological Process | defense response | |
Biological Process | response to salicylic acid | |
Biological Process | response to salt stress |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytosolic sulfotransferase 12
- EC number
- Short namesAtSOT12
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionP52839
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Hypersensitivity to NaCl and ABA in seed germination, and to salicylic acid (SA) in seedling growth.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 37 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000085181 | 1-326 | Cytosolic sulfotransferase 12 | ||
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in the aerial parts of seedlings, in roots, leaves and flowers. Not detected in stems and siliques.
Induction
Up-regulated by pathogens, methyljasmonate, salicylic acid, salt, osmotic stress, cold, auxin, cytokinin and abscisic acid treatments. Not induced by desiccation and ethylene treatment.
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Length326
- Mass (Da)37,139
- Last updated2003-01-10 v2
- Checksum085069A2EBD4E10A
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 85-93 | in Ref. 5; CAA80546 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z46823 EMBL· GenBank· DDBJ | CAA86850.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AC006836 EMBL· GenBank· DDBJ | AAD20078.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | AEC05746.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF375458 EMBL· GenBank· DDBJ | AAK53042.1 EMBL· GenBank· DDBJ | mRNA | ||
AY113050 EMBL· GenBank· DDBJ | AAM47358.1 EMBL· GenBank· DDBJ | mRNA | ||
Z23001 EMBL· GenBank· DDBJ | CAA80546.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |