P52839 · SOT12_ARATH

Function

function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the stereospecific sulfate conjugation of 24-epibrassinosteroids. Preferred substrates are 24-epicathasterone and 6-deoxo-24-epicathasterone. Low activity with 22-deoxy-24-epiteasterone. No activity with 24-epimers catasterone and brassinolide. Sulfonates salicylic acid. May be involved in detoxification. Enhances plant response to pathogen infection and contributes to long distance signaling in systemic acquired resistance (SAR).

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
6.9 μM24-epicathasterone
1.9 μM6-deoxo-24-epicathasterone
3 μM17-beta-estradiol
1.1 μMdehydroepiandrosterone
13 μMpregnenolone
0.44 mMsalicylic acid
3 μM3'-phospho-5'-adenylyl sulfate
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
57 pmol/sec/mgwith 24-epicathasterone as substrate
4.4 pmol/sec/mgwith 24-epicathasterone as substrate
1.6 pmol/sec/mgwith 17-beta-estradiol as substrate
1.3 pmol/sec/mgwith dehydroepiandrosterone as substrate
5.9 pmol/sec/mgwith pregnenolone as substrate
2.67 pmol/sec/mgwith salicylic acid as substrate

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site75-803'-phosphoadenylyl sulfate (UniProtKB | ChEBI)
Active site140Proton acceptor
Binding site1623'-phosphoadenylyl sulfate (UniProtKB | ChEBI)
Binding site1703'-phosphoadenylyl sulfate (UniProtKB | ChEBI)
Binding site2283'-phosphoadenylyl sulfate (UniProtKB | ChEBI)
Binding site290-2923'-phosphoadenylyl sulfate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular ComponentGolgi apparatus
Molecular Functionbrassinosteroid sulfotransferase activity
Molecular Functionflavonoid sulfotransferase activity
Molecular Functionsulfotransferase activity
Biological Processbrassinosteroid metabolic process
Biological Processdefense response
Biological Processresponse to salicylic acid
Biological Processresponse to salt stress

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytosolic sulfotransferase 12
  • EC number
  • Short names
    AtSOT12
  • Alternative names
    • Sulfotransferase 1 (AtST1)

Gene names

    • Name
      SOT12
    • Synonyms
      RAR047, ST1
    • ORF names
      F19B11.21
    • Ordered locus names
      At2g03760

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    P52839
  • Secondary accessions
    • Q9SJW2

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Hypersensitivity to NaCl and ABA in seed germination, and to salicylic acid (SA) in seedling growth.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 37 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000851811-326Cytosolic sulfotransferase 12

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in the aerial parts of seedlings, in roots, leaves and flowers. Not detected in stems and siliques.

Induction

Up-regulated by pathogens, methyljasmonate, salicylic acid, salt, osmotic stress, cold, auxin, cytokinin and abscisic acid treatments. Not induced by desiccation and ethylene treatment.

Gene expression databases

Interaction

Subunit

Dimer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the sulfotransferase 1 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    326
  • Mass (Da)
    37,139
  • Last updated
    2003-01-10 v2
  • Checksum
    085069A2EBD4E10A
MSSSSSVPAYLGDEDLTQETRALISSLPKEKGWLVSEIYEFQGLWHTQAILQGILICQKRFEAKDSDIILVTNPKSGTTWLKALVFALLNRHKFPVSSSGNHPLLVTNPHLLVPFLEGVYYESPDFDFSSLPSPRLMNTHISHLSLPESVKSSSCKIVYCCRNPKDMFVSLWHFGKKLAPEETADYPIEKAVEAFCEGKFIGGPFWDHILEYWYASRENPNKVLFVTYEELKKQTEVEMKRIAEFLECGFIEEEEVREIVKLCSFESLSNLEVNKEGKLPNGIETKTFFRKGEIGGWRDTLSESLAEEIDRTIEEKFKGSGLKFSS

Sequence caution

The sequence CAA80546.1 differs from that shown. Reason: Erroneous initiation Extended N-terminus.
The sequence CAA86850.1 differs from that shown. Reason: Frameshift

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict85-93in Ref. 5; CAA80546

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z46823
EMBL· GenBank· DDBJ
CAA86850.1
EMBL· GenBank· DDBJ
mRNA Frameshift
AC006836
EMBL· GenBank· DDBJ
AAD20078.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002685
EMBL· GenBank· DDBJ
AEC05746.1
EMBL· GenBank· DDBJ
Genomic DNA
AF375458
EMBL· GenBank· DDBJ
AAK53042.1
EMBL· GenBank· DDBJ
mRNA
AY113050
EMBL· GenBank· DDBJ
AAM47358.1
EMBL· GenBank· DDBJ
mRNA
Z23001
EMBL· GenBank· DDBJ
CAA80546.1
EMBL· GenBank· DDBJ
mRNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

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