P52579 · IFRH_TOBAC
- ProteinIsoflavone reductase homolog A622
- GeneA622
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids310 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Involved in the biosynthesis of pyridine alkaloid natural products, leading mainly to the production of anabasine, anatabine, nicotine and nornicotine, effective deterrents against herbivores with antiparasitic and pesticide properties (neurotoxins); nornicotine serves as the precursor in the synthesis of the carcinogen compound N'-nitrosonornicotine (NNN) (PubMed:19002761, PubMed:31276744).
Probable NADPH-dependent oxidoreductase that binds NADPH in vitro. Does not seem to be involved in the NADPH-dependent reduction of phenylcoumaran benzylic ethers (PubMed:12369619).
Reductase that may be involved in a late step of tobacco alkaloid biosynthesis. Maybe involved in either the formation of a nicotinic acid-derived precursor or the final condensation reaction of tobacco alkaloids (PubMed:19002761).
Probable NADPH-dependent oxidoreductase that binds NADPH in vitro. Does not seem to be involved in the NADPH-dependent reduction of phenylcoumaran benzylic ethers (PubMed:12369619).
Reductase that may be involved in a late step of tobacco alkaloid biosynthesis. Maybe involved in either the formation of a nicotinic acid-derived precursor or the final condensation reaction of tobacco alkaloids (PubMed:19002761).
Miscellaneous
Root cells silencing A622 exhibit inhibition of cell growth, severely decreased formation of several alkaloids, and accumulation of nicotinic acid beta-N-glucoside and N-methylpyrrolinium cation.
Pathway
Alkaloid biosynthesis; nicotine biosynthesis.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | NADP binding | |
Molecular Function | oxidoreductase activity | |
Biological Process | alkaloid metabolic process | |
Biological Process | nicotine biosynthetic process | |
Biological Process | response to auxin | |
Biological Process | secondary metabolite biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameIsoflavone reductase homolog A622
- EC number
- Short namesNtA622
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Nicotianoideae > Nicotianeae > Nicotiana
Accessions
- Primary accessionP52579
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000204551 | 1-310 | Isoflavone reductase homolog A622 | |||
Sequence: MVVSEKSKILIIGGTGYIGKYLVETSAKSGHPTFALIRESTLKNPEKSKLIDTFKSYGVTLLFGDISNQESLLKAIKQVDVVISTVGGQQFTDQVNIIKAIKEAGNIKRFLPSEFGFDVDHARAIEPAASLFALKVRIRRMIEAEGIPYTYVICNWFADFFLPNLGQLEAKTPPRDKVVIFGDGNPKAIYVKEEDIATYTIEAVDDPRTLNKTLHMRPPANILSFNEIVSLWEDKIGKTLEKLYLSEEDILQIVQEGPLPLRTNLAICHSVFVNGDSANFEVQPPTGVEATELYPKVKYTTVDEFYNKFV |
Proteomic databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length310
- Mass (Da)34,654
- Last updated1996-10-01 v1
- Checksum03A7F3BB6D451885
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A9B4LJH3 | A0A9B4LJH3_TOBAC | LOC107784748 | 310 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D28505 EMBL· GenBank· DDBJ | BAA05866.1 EMBL· GenBank· DDBJ | mRNA | ||
KJ776595 EMBL· GenBank· DDBJ | AII71783.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KJ776596 EMBL· GenBank· DDBJ | AII71784.1 EMBL· GenBank· DDBJ | mRNA |