P52332 · JAK1_MOUSE

  • Protein
    Tyrosine-protein kinase JAK1
  • Gene
    Jak1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor as well as interleukin (IL)-10 receptor. Kinase partner for the type I interferon receptor IFNAR2. In response to interferon-binding to IFNAR1-IFNAR2 heterodimer, phosphorylates and activates its binding partner IFNAR2, creating docking sites for STAT proteins. Directly phosphorylates STAT proteins but also activates STAT signaling through the transactivation of other JAK kinases associated with signaling receptors.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Mn2+ was used in the in vitro kinase assay but Mg2+ is likely to be the in vivo cofactor.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site880-888ATP (UniProtKB | ChEBI)
Binding site907ATP (UniProtKB | ChEBI)
Active site1002Proton acceptor

GO annotations

AspectTerm
Cellular Componentcytoskeleton
Cellular Componentcytosol
Cellular Componentendomembrane system
Cellular Componentmembrane
Molecular FunctionATP binding
Molecular FunctionCCR5 chemokine receptor binding
Molecular Functiongrowth hormone receptor binding
Molecular Functionmetal ion binding
Molecular Functionnon-membrane spanning protein tyrosine kinase activity
Molecular Functionprotein tyrosine kinase activity
Biological Processcell differentiation
Biological Processcell surface receptor signaling pathway via JAK-STAT
Biological Processcellular response to interleukin-3
Biological Processcellular response to interleukin-7
Biological Processcytokine-mediated signaling pathway
Biological Processenzyme-linked receptor protein signaling pathway
Biological Processgrowth hormone receptor signaling pathway via JAK-STAT
Biological Processinterleukin-2-mediated signaling pathway
Biological Processintracellular signal transduction
Biological Processpositive regulation of receptor signaling pathway via STAT
Biological Processresponse to antibiotic

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Tyrosine-protein kinase JAK1
  • EC number
  • Alternative names
    • Janus kinase 1 (JAK-1)

Gene names

    • Name
      Jak1

Organism names

  • Taxonomic identifier
  • Strain
    • OF1
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P52332
  • Secondary accessions
    • Q62126

Proteomes

Organism-specific databases

Subcellular Location

Endomembrane system ; Peripheral membrane protein
Note: Wholly intracellular, possibly membrane associated.

Keywords

Phenotypes & Variants

PTM/Processing

Features

Showing features for modified residue, chain.

TypeIDPosition(s)Description
Modified residue1N-acetylmethionine
ChainPRO_00000881091-1153Tyrosine-protein kinase JAK1
Modified residue3Phosphotyrosine
Modified residue228Phosphoserine
Modified residue1033Phosphotyrosine; by autocatalysis
Modified residue1034Phosphotyrosine; by autocatalysis

Post-translational modification

Ubiquitinated by RNF125; leading to its degradation by the proteasome.
Autophosphorylated. Phosphorylated on tyrosine residues in response to interferon gamma signaling. Dephosphorylation of Tyr-1034 and Tyr-1034 by PTPN2 negatively regulates cytokine-mediated signaling.

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Interacts with IL31RA. Interacts with IFNAR2. Interacts with IFNGR1. Interacts with JAKMIP1. Interacts with SHB. Interacts (via N-terminus) with IL2RB and IL10RA (via its cytoplasmic domain) (By similarity).
Interacts with FER (PubMed:12738762).

Protein-protein interaction databases

Chemistry

Miscellaneous

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain34-420FERM
Domain439-542SH2
Domain582-854Protein kinase 1
Domain874-1152Protein kinase 2

Domain

The FERM domain mediates interaction with JAKMIP1.
The protein kinase 1 domain is also called the pseudokinase domain and has a regulatory role through the transactivation of other JAK kinases associated with signaling receptors.
The protein kinase 2 domain is the catalytically active domain.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,153
  • Mass (Da)
    133,367
  • Last updated
    1996-10-01 v1
  • Checksum
    0B9816AEA5B3868B
MQYLNIKEDCNAMAFCAKMRSFKKTEVKQVVPEPGVEVTFYLLDREPLRLGSGEYTAEELCIRAAQECSISPLCHNLFALYDESTKLWYAPNRIITVDDKTSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKRVPEATPLLDASSLEYLFAQGQYDLIKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVHDLKVKYLATLETSTLTKHYGAEIFETSMLLISSENELSRCHSNDSGNVLYEVMVTGNLGIQWRQKPNVVPVEKEKNKLKRKKLEYNKHKKDDERNKLREEWNNFSYFPEITHIVIKESVVSINKQDNKNMELKLSSREEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEVLGGQKQFKNFQIEVQKGRYSLHGSMDHFPSLRDLMNHLKKQILRTDNISFVLKRCCQPKPREISNLLVATKKAQEWQPVYSMSQLSFDRILKKDIIQGEHLGRGTRTHIYSGTLLDYKDEEGIAEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDALTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSDIGPFIKLSDPGIPVSVLTRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKQPTTEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICMEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAIQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLIQCKFYIASDVWSFGVTLHELLTYCDSDFSPMALFLKMIGPTHGQMTVTRLVKTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTTFQNLIEGFEALLK

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
B1ASP2B1ASP2_MOUSEJak11153
B1ASP3B1ASP3_MOUSEJak118

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict999in Ref. 3 and 4
Sequence conflict1068-1069in Ref. 3 and 4

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
S63728
EMBL· GenBank· DDBJ
AAB27517.2
EMBL· GenBank· DDBJ
mRNA
M33425
EMBL· GenBank· DDBJ
AAA40016.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp