P52275 · TBB2_CAEEL

  • Protein
    Tubulin beta-2 chain
  • Gene
    tbb-2
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (By similarity).
Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (By similarity).
Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (By similarity).
Required for the normal dynamic behavior of the non-centrosomal microtubules in the epidermal syncytium (PubMed:31995031).
Involved in the redistribution of microtubule end-binding protein EB1/ebp-2 caused by wounding (PubMed:31995031).
Required to modulate expression in the epidermis of antimicrobial peptides, such as nlp-29, after wounding, or fungal infection (PubMed:31995031).

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

145050100150200250300350400450
TypeIDPosition(s)Description
Binding site11GTP (UniProtKB | ChEBI)
Binding site69GTP (UniProtKB | ChEBI)
Binding site69Mg2+ (UniProtKB | ChEBI)
Binding site138GTP (UniProtKB | ChEBI)
Binding site142GTP (UniProtKB | ChEBI)
Binding site143GTP (UniProtKB | ChEBI)
Binding site144GTP (UniProtKB | ChEBI)
Binding site204GTP (UniProtKB | ChEBI)
Binding site226GTP (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentmicrotubule
Cellular Componentspindle microtubule
Cellular Componenttubulin complex
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionmetal ion binding
Molecular Functionstructural constituent of cytoskeleton
Biological Processmeiotic spindle organization
Biological Processmicrotubule cytoskeleton organization
Biological Processmitotic cell cycle

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Tubulin beta-2 chain
  • Alternative names
    • Beta-2-tubulin

Gene names

    • Name
      tbb-2
    • ORF names
      C36E8.5

Organism names

  • Taxonomic identifier
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    P52275

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

RNAi-mediated knockdown abolishes recruitment of microtubule end-binding protein EB1/ebp-2 to a wound site (PubMed:31995031).
Almost completely abolishes induction of nlp-29 expression upon infection with Drechmeria coniospora (PubMed:31995031).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis404Partial reduction in ced-3-mediated cleavage; when associated with E-417 and E-435.
Mutagenesis417Partial reduction in ced-3-mediated cleavage; when associated with E-404 and E-435.
Mutagenesis435Partial reduction in ced-3-mediated cleavage; when associated with E-404 and E-417.
Mutagenesis439In sb26; no effect on thick filament organization in body wall muscles. Partially restores thick filament organization in a mel-26 (ct61sb4) mutant background.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000482871-450Tubulin beta-2 chain

Post-translational modification

Cleaved by caspase ced-3 in vitro.

Proteomic databases

Expression

Gene expression databases

Interaction

Subunit

Dimer of alpha and beta chains (By similarity).
A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nm (By similarity).
Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity (By similarity).
Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells (By similarity).

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region428-450Disordered
Compositional bias432-450Acidic residues

Sequence similarities

Belongs to the tubulin family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    450
  • Mass (Da)
    50,345
  • Last updated
    1996-10-01 v1
  • Checksum
    8F04D0D2AB165006
MREIVHVQAGQCGNQIGSKFWEVISDEHGIQPDGTFKGETDLQLERIDVYYNEANNGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICYRTLKLTNPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGTQAYRALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLISEYQQYQEATAEDDVDGYAEGEAGETYESEQ

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias432-450Acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z35597
EMBL· GenBank· DDBJ
CAA84648.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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