P52026 · DPO1_GEOSE
- ProteinDNA polymerase I
- GenepolA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids876 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
In addition to polymerase activity, the recombinant enzyme has strand displacement and 5'-3' exonuclease activity, but lacks proofreading 3'-5' exonuclease activity.
Miscellaneous
The enzyme from this organism does not have any 3'-5' exonuclease activity. The subtilisin large fragment (residues 289-876) has wild-type polymerase activity but no 5'-3' exonuclease activity.
Catalytic activity
- a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)
Temperature Dependence
Optimum temperature is 65 degrees Celsius.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 3'-5' exonuclease activity | |
Molecular Function | 5'-3' exonuclease activity | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed DNA polymerase activity | |
Biological Process | DNA-templated DNA replication | |
Biological Process | double-strand break repair |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA polymerase I
- EC number
- Short namesPOL I
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Geobacillus
Accessions
- Primary accessionP52026
Phenotypes & Variants
Features
Showing features for mutagenesis, natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 73 | Complete loss of 5'-3' exonuclease activity. | ||||
Sequence: Y → A or P | ||||||
Natural variant | 298 | in strain: X | ||||
Sequence: G → K | ||||||
Natural variant | 300 | in strain: X | ||||
Sequence: D → A | ||||||
Natural variant | 302-303 | in strain: X | ||||
Sequence: AI → TL | ||||||
Natural variant | 306 | in strain: X | ||||
Sequence: S → R | ||||||
Natural variant | 309 | in strain: X | ||||
Sequence: D → E | ||||||
Natural variant | 320 | in strain: X | ||||
Sequence: V → L | ||||||
Natural variant | 324-325 | in strain: X | ||||
Sequence: GD → EE | ||||||
Natural variant | 329 | in strain: X | ||||
Sequence: H → D | ||||||
Natural variant | 337-338 | in strain: X | ||||
Sequence: LA → VV | ||||||
Natural variant | 341 | in strain: X | ||||
Sequence: R → H | ||||||
Natural variant | 356 | in strain: X | ||||
Sequence: K → Q | ||||||
Natural variant | 358 | in strain: X | ||||
Sequence: L → V | ||||||
Natural variant | 369 | in strain: X | ||||
Sequence: T → S | ||||||
Natural variant | 388 | in strain: X | ||||
Sequence: R → C | ||||||
Natural variant | 391 | in strain: X | ||||
Sequence: V → S | ||||||
Natural variant | 406-408 | in strain: X | ||||
Sequence: AAG → GVD | ||||||
Natural variant | 411 | in strain: X | ||||
Sequence: A → R | ||||||
Natural variant | 413 | in strain: X | ||||
Sequence: V → A | ||||||
Natural variant | 417 | in strain: X | ||||
Sequence: H → K | ||||||
Natural variant | 424 | in strain: X | ||||
Sequence: S → P | ||||||
Natural variant | 436 | in strain: X | ||||
Sequence: T → A | ||||||
Natural variant | 442 | in strain: X | ||||
Sequence: T → V | ||||||
Natural variant | 456 | in strain: X | ||||
Sequence: A → E | ||||||
Natural variant | 459 | in strain: X | ||||
Sequence: E → R | ||||||
Natural variant | 461-462 | in strain: X | ||||
Sequence: LM → FL | ||||||
Natural variant | 475 | in strain: X | ||||
Sequence: T → V | ||||||
Natural variant | 482-483 | in strain: X | ||||
Sequence: AG → SS | ||||||
Natural variant | 487 | in strain: X | ||||
Sequence: N → E | ||||||
Natural variant | 491 | in strain: X | ||||
Sequence: T → A | ||||||
Natural variant | 505 | in strain: X | ||||
Sequence: A → K | ||||||
Natural variant | 508 | in strain: X | ||||
Sequence: T → R | ||||||
Natural variant | 510 | in strain: X | ||||
Sequence: Q → K | ||||||
Natural variant | 512-513 | in strain: X | ||||
Sequence: QA → GT | ||||||
Natural variant | 516 | in strain: X | ||||
Sequence: R → Q | ||||||
Natural variant | 536-537 | in strain: X | ||||
Sequence: TV → VI | ||||||
Natural variant | 540 | in strain: X | ||||
Sequence: D → E | ||||||
Natural variant | 567 | in strain: X | ||||
Sequence: H → Y | ||||||
Natural variant | 573 | in strain: X | ||||
Sequence: H → N | ||||||
Natural variant | 596 | in strain: X | ||||
Sequence: H → R | ||||||
Natural variant | 598 | in strain: X | ||||
Sequence: V → D | ||||||
Natural variant | 600 | in strain: X | ||||
Sequence: G → K | ||||||
Natural variant | 605 | in strain: X | ||||
Sequence: M → I | ||||||
Natural variant | 619 | in strain: X | ||||
Sequence: V → T | ||||||
Natural variant | 645 | in strain: X | ||||
Sequence: P → S | ||||||
Natural variant | 672 | in strain: X | ||||
Sequence: I → M | ||||||
Natural variant | 678 | in strain: X | ||||
Sequence: G → D | ||||||
Natural variant | 691 | in strain: X | ||||
Sequence: H → Q | ||||||
Natural variant | 695-696 | in strain: X | ||||
Sequence: ED → DE | ||||||
Natural variant | 699 | in strain: X | ||||
Sequence: A → P | ||||||
Natural variant | 728 | in strain: X | ||||
Sequence: T → S | ||||||
Natural variant | 741 | in strain: X | ||||
Sequence: A → E | ||||||
Natural variant | 748 | in strain: X | ||||
Sequence: Q → R | ||||||
Natural variant | 751 | in strain: X | ||||
Sequence: D → E | ||||||
Natural variant | 790 | in strain: X | ||||
Sequence: T → M | ||||||
Natural variant | 812-813 | in strain: X | ||||
Sequence: SV → NA | ||||||
Natural variant | 816 | in strain: X | ||||
Sequence: R → K | ||||||
Natural variant | 841 | in strain: X | ||||
Sequence: I → M | ||||||
Natural variant | 870 | in strain: X | ||||
Sequence: P → S |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 58 variants from UniProt as well as other sources including ClinVar and dbSNP.
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000101234 | 1-876 | DNA polymerase I | |||
Sequence: MKNKLVLIDGNSVAYRAFFALPLLHNDKGIHTNAVYGFTMMLNKILAEEQPTHILVAFDAGKTTFRHETFQDYKGGRQQTPPELSEQFPLLRELLKAYRIPAYELDHYEADDIIGTMAARAEREGFAVKVISGDRDLTQLASPQVTVEITKKGITDIESYTPETVVEKYGLTPEQIVDLKGLMGDKSDNIPGVPGIGEKTAVKLLKQFGTVENVLASIDEIKGEKLKENLRQYRDLALLSKQLAAICRDAPVELTLDDIVYKGEDREKVVALFQELGFQSFLDKMAVQTDEGEKPLAGMDFAIADSVTDEMLADKAALVVEVVGDNYHHAPIVGIALANERGRFFLRPETALADPKFLAWLGDETKKKTMFDSKRAAVALKWKGIELRGVVFDLLLAAYLLDPAQAAGDVAAVAKMHQYEAVRSDEAVYGKGAKRTVPDEPTLAEHLVRKAAAIWALEEPLMDELRRNEQDRLLTELEQPLAGILANMEFTGVKVDTKRLEQMGAELTEQLQAVERRIYELAGQEFNINSPKQLGTVLFDKLQLPVLKKTKTGYSTSADVLEKLAPHHEIVEHILHYRQLGKLQSTYIEGLLKVVHPVTGKVHTMFNQALTQTGRLSSVEPNLQNIPIRLEEGRKIRQAFVPSEPDWLIFAADYSQIELRVLAHIAEDDNLIEAFRRGLDIHTKTAMDIFHVSEEDVTANMRRQAKAVNFGIVYGISDYGLAQNLNITRKEAAEFIERYFASFPGVKQYMDNIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERTAMNTPIQGSAADIIKKAMIDLSVRLREERLQARLLLQVHDELILEAPKEEIERLCRLVPEVMEQAVTLRVPLKVDYHYGPTWYDAK |
Interaction
Subunit
Single-chain monomer with multiple functions.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-310 | 5'-3' exonuclease | ||||
Sequence: MKNKLVLIDGNSVAYRAFFALPLLHNDKGIHTNAVYGFTMMLNKILAEEQPTHILVAFDAGKTTFRHETFQDYKGGRQQTPPELSEQFPLLRELLKAYRIPAYELDHYEADDIIGTMAARAEREGFAVKVISGDRDLTQLASPQVTVEITKKGITDIESYTPETVVEKYGLTPEQIVDLKGLMGDKSDNIPGVPGIGEKTAVKLLKQFGTVENVLASIDEIKGEKLKENLRQYRDLALLSKQLAAICRDAPVELTLDDIVYKGEDREKVVALFQELGFQSFLDKMAVQTDEGEKPLAGMDFAIADSVTDE | ||||||
Region | 289-876 | Subtilisin large fragment | ||||
Sequence: TDEGEKPLAGMDFAIADSVTDEMLADKAALVVEVVGDNYHHAPIVGIALANERGRFFLRPETALADPKFLAWLGDETKKKTMFDSKRAAVALKWKGIELRGVVFDLLLAAYLLDPAQAAGDVAAVAKMHQYEAVRSDEAVYGKGAKRTVPDEPTLAEHLVRKAAAIWALEEPLMDELRRNEQDRLLTELEQPLAGILANMEFTGVKVDTKRLEQMGAELTEQLQAVERRIYELAGQEFNINSPKQLGTVLFDKLQLPVLKKTKTGYSTSADVLEKLAPHHEIVEHILHYRQLGKLQSTYIEGLLKVVHPVTGKVHTMFNQALTQTGRLSSVEPNLQNIPIRLEEGRKIRQAFVPSEPDWLIFAADYSQIELRVLAHIAEDDNLIEAFRRGLDIHTKTAMDIFHVSEEDVTANMRRQAKAVNFGIVYGISDYGLAQNLNITRKEAAEFIERYFASFPGVKQYMDNIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERTAMNTPIQGSAADIIKKAMIDLSVRLREERLQARLLLQVHDELILEAPKEEIERLCRLVPEVMEQAVTLRVPLKVDYHYGPTWYDAK | ||||||
Region | 469-876 | Polymerase | ||||
Sequence: EQDRLLTELEQPLAGILANMEFTGVKVDTKRLEQMGAELTEQLQAVERRIYELAGQEFNINSPKQLGTVLFDKLQLPVLKKTKTGYSTSADVLEKLAPHHEIVEHILHYRQLGKLQSTYIEGLLKVVHPVTGKVHTMFNQALTQTGRLSSVEPNLQNIPIRLEEGRKIRQAFVPSEPDWLIFAADYSQIELRVLAHIAEDDNLIEAFRRGLDIHTKTAMDIFHVSEEDVTANMRRQAKAVNFGIVYGISDYGLAQNLNITRKEAAEFIERYFASFPGVKQYMDNIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERTAMNTPIQGSAADIIKKAMIDLSVRLREERLQARLLLQVHDELILEAPKEEIERLCRLVPEVMEQAVTLRVPLKVDYHYGPTWYDAK |
Sequence similarities
Belongs to the DNA polymerase type-A family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length876
- Mass (Da)98,670
- Last updated1998-07-15 v2
- Checksum689167A801D543E4
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 91 | in Ref. 2; AAA85558 | ||||
Sequence: L → V | ||||||
Sequence conflict | 198 | in Ref. 2; AAA85558 | ||||
Sequence: E → K | ||||||
Sequence conflict | 352 | in Ref. 2; AAA85558 | ||||
Sequence: L → V | ||||||
Sequence conflict | 381-382 | in Ref. 2; AAA85558 | ||||
Sequence: KW → NG | ||||||
Sequence conflict | 388 | in Ref. 2; AAA85558 | ||||
Sequence: R → AGV | ||||||
Sequence conflict | 446 | in Ref. 2; AAA85558 | ||||
Sequence: H → Q | ||||||
Sequence conflict | 448 | in Ref. 1; AAC37139 | ||||
Sequence: V → A | ||||||
Sequence conflict | 479-480 | in Ref. 2; AAA85558 | ||||
Sequence: QP → HA | ||||||
Sequence conflict | 678 | in Ref. 2; AAA85558 | ||||
Sequence: G → W | ||||||
Sequence conflict | 785 | in Ref. 2; AAA85558 | ||||
Sequence: S → T | ||||||
Sequence conflict | 814-815 | in Ref. 2; AAA85558 | ||||
Sequence: RL → SV | ||||||
Sequence conflict | 828 | in Ref. 2; AAA85558 | ||||
Sequence: V → G | ||||||
Sequence conflict | 842 | in Ref. 2; AAA85558 | ||||
Sequence: E → G | ||||||
Sequence conflict | 857 | in Ref. 1; AAC37139 | ||||
Sequence: T → A |
Keywords
- Technical term