P51996 · YPT32_YEAST
- ProteinGTP-binding protein YPT32/YPT11
- GeneYPT32
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids222 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Required for protein transport through the secretory pathway. Probably involved in regulation of secretory vesicle formation at the trans-Golgi compartment. Mediates the proper polarized localization of SEC2, a GEF for SEC4, but does not alter the exchange activity of SEC2 on SEC4. Plays a role in autophagy.
Miscellaneous
Present with 4298 molecules/cell in log phase SD medium.
Activity regulation
Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) TRAPP complex, and inactivated by GTPase-activating protein (GAP) GYP3.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cellular bud neck | |
Cellular Component | cytosol | |
Cellular Component | endosome | |
Cellular Component | Golgi apparatus | |
Cellular Component | Golgi membrane | |
Cellular Component | mitochondrial outer membrane | |
Cellular Component | recycling endosome | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Biological Process | autophagy | |
Biological Process | early endosome to Golgi transport | |
Biological Process | exocytosis | |
Biological Process | protein transport | |
Biological Process | vesicle-mediated transport |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGTP-binding protein YPT32/YPT11
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP51996
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 27 | Binds preferentially GDP. | ||||
Sequence: S → N | ||||||
Mutagenesis | 49 | Cold sensitive. | ||||
Sequence: E → Q | ||||||
Mutagenesis | 72 | Reduces GTPase activity. | ||||
Sequence: Q → L | ||||||
Mutagenesis | 126 | Blocks nucleotide binding. | ||||
Sequence: N → I | ||||||
Mutagenesis | 141 | Loss of function at 37 degrees Celsius. | ||||
Sequence: A → D |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain, lipidation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylserine | ||||
Sequence: S | ||||||
Chain | PRO_0000121324 | 2-222 | GTP-binding protein YPT32/YPT11 | |||
Sequence: SNEDYGYDYDYLFKIVLIGDSGVGKSNLLSRFTTDEFNIESKSTIGVEFATRTIEVENKKIKAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKSSSYENCNHWLTELRENADDNVAVGLIGNKSDLAHLRAVPTDEAKNFAMENQMLFTETSALNSDNVDKAFRELIVAIFQMVSKHQVDLSGSGTNNMGSNGAPKGPTISLTPAPKEDKKKKSSNCC | ||||||
Lipidation | 221 | S-geranylgeranyl cysteine | ||||
Sequence: C | ||||||
Lipidation | 222 | S-geranylgeranyl cysteine | ||||
Sequence: C |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Interacts with YIF1, YIP3, YIP4 and YIP5. Interacts with the GEF SEC2. Interacts with TRS130.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P51996 | GDI1 P39958 | 4 | EBI-29384, EBI-7517 | |
BINARY | P51996 | GYP1 Q08484 | 3 | EBI-29384, EBI-8005 | |
BINARY | P51996 | YIF1 P53845 | 2 | EBI-29384, EBI-28230 | |
BINARY | P51996 | YIP3 P53633 | 2 | EBI-29384, EBI-25301 | |
BINARY | P51996 | YIP4 P53093 | 2 | EBI-29384, EBI-24124 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for motif, compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 42-50 | Effector region | ||||
Sequence: SKSTIGVEF | ||||||
Compositional bias | 186-202 | Polar residues | ||||
Sequence: SGSGTNNMGSNGAPKGP | ||||||
Region | 186-222 | Disordered | ||||
Sequence: SGSGTNNMGSNGAPKGPTISLTPAPKEDKKKKSSNCC |
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length222
- Mass (Da)24,520
- Last updated2007-01-23 v3
- Checksum621217A296161964
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 186-202 | Polar residues | ||||
Sequence: SGSGTNNMGSNGAPKGP |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X72834 EMBL· GenBank· DDBJ | CAA51355.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U33754 EMBL· GenBank· DDBJ | AAC49495.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z72732 EMBL· GenBank· DDBJ | CAA96926.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY558506 EMBL· GenBank· DDBJ | AAS56832.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006941 EMBL· GenBank· DDBJ | DAA07906.1 EMBL· GenBank· DDBJ | Genomic DNA |