P51796 · CLCN5_RAT
- ProteinH(+)/Cl(-) exchange transporter 5
- GeneClcn5
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids816 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function (By similarity).
The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels (Probable)
The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels (Probable)
Catalytic activity
- 2 chloride(in) + H+(out) = 2 chloride(out) + H+(in)
2 chloride (in)CHEBI:17996+ H+ (out)CHEBI:15378= 2 chloride (out)CHEBI:17996+ H+ (in)CHEBI:15378
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 238 | chloride (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Site | 281 | Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H+ and Cl- transport | ||||
Sequence: E | ||||||
Site | 338 | Mediates proton transfer from the protein to the inner aqueous phase | ||||
Sequence: E | ||||||
Binding site | 525 | chloride (UniProtKB | ChEBI) | ||||
Sequence: F | ||||||
Binding site | 628 | chloride (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 666 | ATP (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 687-689 | ATP (UniProtKB | ChEBI) | ||||
Sequence: YSG | ||||||
Binding site | 794-797 | ATP (UniProtKB | ChEBI) | ||||
Sequence: TKKD |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | apical part of cell | |
Cellular Component | cytosol | |
Cellular Component | early endosome | |
Cellular Component | endosome | |
Cellular Component | endosome membrane | |
Cellular Component | Golgi apparatus | |
Cellular Component | Golgi membrane | |
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Cellular Component | synaptic vesicle | |
Molecular Function | antiporter activity | |
Molecular Function | ATP binding | |
Molecular Function | identical protein binding | |
Molecular Function | voltage-gated chloride channel activity | |
Biological Process | chloride transport | |
Biological Process | endocytosis | |
Biological Process | renal system process |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameH(+)/Cl(-) exchange transporter 5
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionP51796
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Multi-pass membrane protein
Endosome membrane ; Multi-pass membrane protein
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane, intramembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-124 | Cytoplasmic | ||||
Sequence: MAMWQGAMDNRGFHQGSFSSFQSSSSDEDLMDIPGTAMDFSMRDDVPPLDREIEGNKSYNGGGIGSSNRIMDFLEEPIPGVGTYDDFNTIDWVREKSRDRDRHREITNKSKESTWALIHSVSDA | ||||||
Transmembrane | 125-162 | Helical | ||||
Sequence: FSGWLLMLLIGLLSGSLAGLIDISAHWMTDLKEGICTG | ||||||
Transmembrane | 208-231 | Helical | ||||
Sequence: VNYFMYVLWALLFAFLAVSLVKAF | ||||||
Intramembrane | 240-247 | Helical | ||||
Sequence: IPEIKTIL | ||||||
Transmembrane | 256-275 | Helical | ||||
Sequence: LGKWTLVIKTITLVLAVSSG | ||||||
Transmembrane | 281-300 | Helical | ||||
Sequence: EGPLVHVACCCGNILCHCFN | ||||||
Intramembrane | 312-324 | Helical | ||||
Sequence: VLSAAAAAGVSVA | ||||||
Intramembrane | 328-336 | Helical | ||||
Sequence: PIGGVLFSL | ||||||
Transmembrane | 348-366 | Helical | ||||
Sequence: LWRSFFAALVAAFTLRSIN | ||||||
Transmembrane | 389-414 | Helical | ||||
Sequence: LVPFIVLGIFGGLWGALFIRTNIAWC | ||||||
Transmembrane | 422-442 | Helical | ||||
Sequence: LGKYPVVEVLIVTAITAILAF | ||||||
Transmembrane | 498-518 | Helical | ||||
Sequence: MWQLALTLILKIVITIFTFGM | ||||||
Transmembrane | 523-542 | Helical | ||||
Sequence: GLFIPSMAVGAIAGRLLGVG | ||||||
Intramembrane | 570-584 | Helical | ||||
Sequence: GLYAMVGAAACLGGV | ||||||
Intramembrane | 585-587 | Note=Loop between two helices | ||||
Sequence: TRM | ||||||
Intramembrane | 588-599 | Helical | ||||
Sequence: TVSLVVIMFELT | ||||||
Intramembrane | 600-604 | Note=Loop between two helices | ||||
Sequence: GGLEY | ||||||
Transmembrane | 605-622 | Helical | ||||
Sequence: IVPLMAAAMTSKWVADAL | ||||||
Topological domain | 623-816 | Cytoplasmic | ||||
Sequence: GREGIYDAHIRLNGYPFLEAKEEFAHKTLAMDVMKPRRNDPLLTVLTQDSMTVEDVETIISETTYSGFPVVVSRESQRLVGFVLRRDLIISIENARKKQDGVVSTSIIYFTEHSPPMPPYTPPTLKLRNILDLSPFTVTDLTPMEIVVDIFRKLGLRQCLVTHNGRLLGIITKKDVLKHIAQMANQDPESILFN |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000094448 | 1-816 | H+/Cl- exchange transporter 5 | |||
Sequence: MAMWQGAMDNRGFHQGSFSSFQSSSSDEDLMDIPGTAMDFSMRDDVPPLDREIEGNKSYNGGGIGSSNRIMDFLEEPIPGVGTYDDFNTIDWVREKSRDRDRHREITNKSKESTWALIHSVSDAFSGWLLMLLIGLLSGSLAGLIDISAHWMTDLKEGICTGGFWFNHEHCCWNSEHVTFEDRDKCPEWNSWSQLIISTDQGAFAYIVNYFMYVLWALLFAFLAVSLVKAFAPYACGSGIPEIKTILSGFIIRGYLGKWTLVIKTITLVLAVSSGLSLGKEGPLVHVACCCGNILCHCFNKYRKNEAKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEFHTPWHLFELVPFIVLGIFGGLWGALFIRTNIAWCRKRKTTQLGKYPVVEVLIVTAITAILAFPNEYTRMSTSELISELFNDCGLLDSSKLCDYENHFNTSKGGELPDRPAGVGVYSAMWQLALTLILKIVITIFTFGMKIPSGLFIPSMAVGAIAGRLLGVGMEQLAYYHHDWGIFNSWCSQGADCITPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAMTSKWVADALGREGIYDAHIRLNGYPFLEAKEEFAHKTLAMDVMKPRRNDPLLTVLTQDSMTVEDVETIISETTYSGFPVVVSRESQRLVGFVLRRDLIISIENARKKQDGVVSTSIIYFTEHSPPMPPYTPPTLKLRNILDLSPFTVTDLTPMEIVVDIFRKLGLRQCLVTHNGRLLGIITKKDVLKHIAQMANQDPESILFN |
Post-translational modification
Ubiquitinated by NEDD4L in the presence of albumin; which promotes endocytosis and proteasomal degradation.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Interaction
Structure
Family & Domains
Features
Showing features for motif, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 237-241 | Selectivity filter part_1 | ||||
Sequence: GSGIP | ||||||
Motif | 279-283 | Selectivity filter part_2 | ||||
Sequence: GKEGP | ||||||
Motif | 523-527 | Selectivity filter part_3 | ||||
Sequence: GLFIP | ||||||
Domain | 656-720 | CBS 1 | ||||
Sequence: MKPRRNDPLLTVLTQDSMTVEDVETIISETTYSGFPVVVSRESQRLVGFVLRRDLIISIENARKK | ||||||
Domain | 752-811 | CBS 2 | ||||
Sequence: ILDLSPFTVTDLTPMEIVVDIFRKLGLRQCLVTHNGRLLGIITKKDVLKHIAQMANQDPE |
Sequence similarities
Belongs to the chloride channel (TC 2.A.49) family. ClC-5/CLCN5 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
P51796-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length816
- Mass (Da)90,687
- Last updated2020-08-12 v2
- Checksum68AE3812E820141D
P51796-2
- Name2
- Differences from canonical
- 1-70: Missing
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8I5ZVV0 | A0A8I5ZVV0_RAT | Clcn5 | 813 |
Features
Showing features for alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_060659 | 1-70 | in isoform 2 | |||
Sequence: Missing | ||||||
Sequence conflict | 385 | in Ref. 2; BAA09091 | ||||
Sequence: H → Y |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z56277 EMBL· GenBank· DDBJ | CAA91216.1 EMBL· GenBank· DDBJ | mRNA | ||
D50497 EMBL· GenBank· DDBJ | BAA09091.1 EMBL· GenBank· DDBJ | mRNA | ||
AABR07037207 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AABR07037208 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |