P51150 · RAB7A_MOUSE

  • Protein
    Ras-related protein Rab-7a
  • Gene
    Rab7a
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transporter-mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and Mycobacteria. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation (By similarity).
Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (PubMed:23708524).
Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).
Required for vesicular trafficking and cell surface expression of ACE2 (By similarity).
May play a role in PRPH neuronal intermediate filament assembly (PubMed:23179371).

Catalytic activity

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site15-22GTP (UniProtKB | ChEBI)
Binding site34-40GTP (UniProtKB | ChEBI)
Binding site63-67GTP (UniProtKB | ChEBI)
Binding site125-128GTP (UniProtKB | ChEBI)
Binding site156-157GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentalveolar lamellar body
Cellular Componentautophagosome membrane
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentendosome
Cellular Componentglutamatergic synapse
Cellular ComponentGolgi apparatus
Cellular Componentlate endosome
Cellular Componentlate endosome membrane
Cellular Componentlipid droplet
Cellular Componentlysosomal membrane
Cellular Componentlysosome
Cellular Componentmelanosome membrane
Cellular Componentmitochondrial membrane
Cellular Componentmitochondrion
Cellular Componentphagocytic vesicle
Cellular Componentphagocytic vesicle membrane
Cellular Componentphagophore assembly site membrane
Cellular Componentretromer complex
Cellular Componentsynaptic vesicle membrane
Molecular FunctionG protein activity
Molecular FunctionGDP binding
Molecular FunctionGTP binding
Molecular Functionretromer complex binding
Molecular Functionsmall GTPase binding
Biological Processautophagosome assembly
Biological Processbone resorption
Biological Processearly endosome to late endosome transport
Biological Processendosome to lysosome transport
Biological Processendosome to plasma membrane protein transport
Biological Processepidermal growth factor catabolic process
Biological Processestablishment of vesicle localization
Biological Processintracellular protein transport
Biological Processlipid catabolic process
Biological Processlipophagy
Biological Processnegative regulation of exosomal secretion
Biological Processneurotransmitter receptor transport, postsynaptic endosome to lysosome
Biological Processphagosome acidification
Biological Processphagosome-lysosome fusion
Biological Processpositive regulation of exosomal secretion
Biological Processpositive regulation of protein catabolic process
Biological Processpositive regulation of viral process
Biological Processprotein targeting to lysosome
Biological Processprotein to membrane docking
Biological Processresponse to bacterium
Biological Processretrograde transport, endosome to Golgi
Biological Processvesicle-mediated transport in synapse
Biological Processviral release from host cell

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ras-related protein Rab-7a
  • EC number

Gene names

    • Name
      Rab7a
    • Synonyms
      Rab7

Organism names

  • Taxonomic identifier
  • Strains
    • C57BL/6J
    • FVB/N
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P51150

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasmic vesicle, phagosome membrane
; Peripheral membrane protein
Late endosome membrane
; Peripheral membrane protein
Lysosome membrane
; Peripheral membrane protein
Melanosome membrane
; Peripheral membrane protein
Cytoplasmic vesicle, autophagosome membrane
; Peripheral membrane protein
Lipid droplet
Endosome membrane
Cytoplasmic vesicle
Mitochondrion membrane
; Peripheral membrane protein
Note: Colocalizes with OSBPL1A at the late endosome (By similarity).
Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts (By similarity).
Recruited to phagosomes containing S.aureus or Mycobacterium (By similarity).
Lipid droplet localization is increased upon ADRB2 stimulation (PubMed:23708524).
Recruited to damaged mitochondria during mitophagy in a RIMOC1-dependent manner (By similarity).

Keywords

Phenotypes & Variants

Disruption phenotype

Reduces both the basal and, to a greater degree, agonist-stimulated glycerol releases. the ADRB2-stimulated liposlysis.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain, cross-link, lipidation.

TypeIDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylthreonine
ChainPRO_00001211222-207
Modified residue72Phosphoserine
Cross-link191Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Cross-link194Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Lipidation205S-geranylgeranyl cysteine
Modified residue207Cysteine methyl ester
Lipidation207S-geranylgeranyl cysteine

Post-translational modification

Deubiquitination at Lys-191 and Lys-194 by USP32.
Phosphorylated at Ser-72 by LRRK1; phosphorylation is dependent on protein kinase C (PKC) activation of LRRK1.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Widely expressed. High expression in liver, heart and kidney. Found in sensory and motor neurons.

Developmental stage

Expressed in the dorsal root ganglia and the sciatic nerve at 13.5 dpc (at protein level).

Gene expression databases

Interaction

Subunit

Interacts with NTRK1/TRKA (By similarity), RILP (By similarity), PSMA7 (By similarity), RNF115 (PubMed:12972561) and FYCO1 (By similarity).
Interacts with the PIK3C3/VPS34-PIK3R4 complex (By similarity).
The GTP-bound form interacts with OSBPL1A and RAC1 (By similarity).
Interacts with CLN3 (By similarity).
Interacts with CHM, the substrate-binding subunit of the Rab geranylgeranyltransferase complex (By similarity).
Interacts with C9orf72 (PubMed:24549040).
Does not interact with HPS4 and the BLOC-3 complex (heterodimer of HPS1 and HPS4) (PubMed:20048159).
Interacts with CLN5 (By similarity).
Interacts with PLEKHM1 (via N- and C-terminus) (PubMed:27777970).
Interacts with RUFY4 (By similarity).
Interacts with PRPH; the interaction is direct (PubMed:23179371).
Interacts with VPS13A (By similarity).
The GDP-bound form interacts with RIMOC1 (By similarity).
Interacts with the MON1A-CCZ1B complex and this interaction is enhanced in the presence of RIMOC1 (By similarity).
Interacts with VPS39 and VPS41 (PubMed:28063257).

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif37-45Effector region

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    207
  • Mass (Da)
    23,490
  • Last updated
    2004-04-13 v2
  • Checksum
    A2AF33B16A672971
MTSRKKVLLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVMVDDRLVTMQIWDTAGQERFQSLGVAFYRGADCCVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAKASAESCSC

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0N4SVG9A0A0N4SVG9_MOUSERab798
A0A0N4SVR6A0A0N4SVR6_MOUSERab788

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict145in Ref. 1; CAA61797

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X89650
EMBL· GenBank· DDBJ
CAA61797.1
EMBL· GenBank· DDBJ
mRNA
AK005004
EMBL· GenBank· DDBJ
BAB23738.1
EMBL· GenBank· DDBJ
mRNA
BC004597
EMBL· GenBank· DDBJ
AAH04597.1
EMBL· GenBank· DDBJ
mRNA
BC086793
EMBL· GenBank· DDBJ
AAH86793.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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