P51150 · RAB7A_MOUSE
- ProteinRas-related protein Rab-7a
- GeneRab7a
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids207 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transporter-mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and Mycobacteria. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation (By similarity).
Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (PubMed:23708524).
Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).
Required for vesicular trafficking and cell surface expression of ACE2 (By similarity).
May play a role in PRPH neuronal intermediate filament assembly (PubMed:23179371).
Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (PubMed:23708524).
Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).
Required for vesicular trafficking and cell surface expression of ACE2 (By similarity).
May play a role in PRPH neuronal intermediate filament assembly (PubMed:23179371).
Catalytic activity
- GTP + H2O = GDP + H+ + phosphateThis reaction proceeds in the forward direction.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 15-22 | GTP (UniProtKB | ChEBI) | ||||
Sequence: GDSGVGKT | ||||||
Binding site | 34-40 | GTP (UniProtKB | ChEBI) | ||||
Sequence: SNQYKAT | ||||||
Binding site | 63-67 | GTP (UniProtKB | ChEBI) | ||||
Sequence: DTAGQ | ||||||
Binding site | 125-128 | GTP (UniProtKB | ChEBI) | ||||
Sequence: NKID | ||||||
Binding site | 156-157 | GTP (UniProtKB | ChEBI) | ||||
Sequence: AK |
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRas-related protein Rab-7a
- EC number
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP51150
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cytoplasmic vesicle, phagosome membrane ; Peripheral membrane protein
Late endosome membrane ; Peripheral membrane protein
Lysosome membrane ; Peripheral membrane protein
Melanosome membrane ; Peripheral membrane protein
Cytoplasmic vesicle, autophagosome membrane ; Peripheral membrane protein
Mitochondrion membrane ; Peripheral membrane protein
Note: Colocalizes with OSBPL1A at the late endosome (By similarity).
Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts (By similarity).
Recruited to phagosomes containing S.aureus or Mycobacterium (By similarity).
Lipid droplet localization is increased upon ADRB2 stimulation (PubMed:23708524).
Recruited to damaged mitochondria during mitophagy in a RIMOC1-dependent manner (By similarity).
Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts (By similarity).
Recruited to phagosomes containing S.aureus or Mycobacterium (By similarity).
Lipid droplet localization is increased upon ADRB2 stimulation (PubMed:23708524).
Recruited to damaged mitochondria during mitophagy in a RIMOC1-dependent manner (By similarity).
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Reduces both the basal and, to a greater degree, agonist-stimulated glycerol releases. the ADRB2-stimulated liposlysis.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain, cross-link, lipidation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylthreonine | ||||
Sequence: T | ||||||
Chain | PRO_0000121122 | 2-207 | Ras-related protein Rab-7a | |||
Sequence: TSRKKVLLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVMVDDRLVTMQIWDTAGQERFQSLGVAFYRGADCCVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAKASAESCSC | ||||||
Modified residue | 72 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 191 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K | ||||||
Cross-link | 194 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K | ||||||
Lipidation | 205 | S-geranylgeranyl cysteine | ||||
Sequence: C | ||||||
Modified residue | 207 | Cysteine methyl ester | ||||
Sequence: C | ||||||
Lipidation | 207 | S-geranylgeranyl cysteine | ||||
Sequence: C |
Post-translational modification
Deubiquitination at Lys-191 and Lys-194 by USP32.
Phosphorylated at Ser-72 by LRRK1; phosphorylation is dependent on protein kinase C (PKC) activation of LRRK1.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Widely expressed. High expression in liver, heart and kidney. Found in sensory and motor neurons.
Developmental stage
Expressed in the dorsal root ganglia and the sciatic nerve at 13.5 dpc (at protein level).
Gene expression databases
Interaction
Subunit
Interacts with NTRK1/TRKA (By similarity), RILP (By similarity), PSMA7 (By similarity), RNF115 (PubMed:12972561) and FYCO1 (By similarity).
Interacts with the PIK3C3/VPS34-PIK3R4 complex (By similarity).
The GTP-bound form interacts with OSBPL1A and RAC1 (By similarity).
Interacts with CLN3 (By similarity).
Interacts with CHM, the substrate-binding subunit of the Rab geranylgeranyltransferase complex (By similarity).
Interacts with C9orf72 (PubMed:24549040).
Does not interact with HPS4 and the BLOC-3 complex (heterodimer of HPS1 and HPS4) (PubMed:20048159).
Interacts with CLN5 (By similarity).
Interacts with PLEKHM1 (via N- and C-terminus) (PubMed:27777970).
Interacts with RUFY4 (By similarity).
Interacts with PRPH; the interaction is direct (PubMed:23179371).
Interacts with VPS13A (By similarity).
The GDP-bound form interacts with RIMOC1 (By similarity).
Interacts with the MON1A-CCZ1B complex and this interaction is enhanced in the presence of RIMOC1 (By similarity).
Interacts with VPS39 and VPS41 (PubMed:28063257).
Interacts with the PIK3C3/VPS34-PIK3R4 complex (By similarity).
The GTP-bound form interacts with OSBPL1A and RAC1 (By similarity).
Interacts with CLN3 (By similarity).
Interacts with CHM, the substrate-binding subunit of the Rab geranylgeranyltransferase complex (By similarity).
Interacts with C9orf72 (PubMed:24549040).
Does not interact with HPS4 and the BLOC-3 complex (heterodimer of HPS1 and HPS4) (PubMed:20048159).
Interacts with CLN5 (By similarity).
Interacts with PLEKHM1 (via N- and C-terminus) (PubMed:27777970).
Interacts with RUFY4 (By similarity).
Interacts with PRPH; the interaction is direct (PubMed:23179371).
Interacts with VPS13A (By similarity).
The GDP-bound form interacts with RIMOC1 (By similarity).
Interacts with the MON1A-CCZ1B complex and this interaction is enhanced in the presence of RIMOC1 (By similarity).
Interacts with VPS39 and VPS41 (PubMed:28063257).
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 37-45 | Effector region | ||||
Sequence: YKATIGADF |
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length207
- Mass (Da)23,490
- Last updated2004-04-13 v2
- ChecksumA2AF33B16A672971
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0N4SVG9 | A0A0N4SVG9_MOUSE | Rab7 | 98 | ||
A0A0N4SVR6 | A0A0N4SVR6_MOUSE | Rab7 | 88 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 145 | in Ref. 1; CAA61797 | ||||
Sequence: S → R |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X89650 EMBL· GenBank· DDBJ | CAA61797.1 EMBL· GenBank· DDBJ | mRNA | ||
AK005004 EMBL· GenBank· DDBJ | BAB23738.1 EMBL· GenBank· DDBJ | mRNA | ||
BC004597 EMBL· GenBank· DDBJ | AAH04597.1 EMBL· GenBank· DDBJ | mRNA | ||
BC086793 EMBL· GenBank· DDBJ | AAH86793.1 EMBL· GenBank· DDBJ | mRNA |