P50527 · STE20_SCHPO

Function

function

MAP4K component of the MAPK pathway required for the mating pheromone response. Phosphorylates histone H2B to form H2BS10ph (By similarity).
Phosphorylates tea1. Required for skb1-dependent mitotic inhibitory function. Regulates microtubule dynamics and cell polarity

Catalytic activity

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site392-400ATP (UniProtKB | ChEBI)
Binding site415ATP (UniProtKB | ChEBI)
Active site505Proton acceptor

GO annotations

AspectTerm
Cellular ComponentCdc42 GTPase complex
Cellular Componentcell cortex of cell tip
Cellular Componentcell division site
Cellular Componentcell tip
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentmitotic actomyosin contractile ring
Cellular Componentmitotic actomyosin contractile ring, intermediate layer
Cellular Componentspindle microtubule
Molecular FunctionATP binding
Molecular Functionprotein serine kinase activity
Molecular Functionprotein serine/threonine kinase activity
Biological Processactin filament organization
Biological Processintracellular signal transduction
Biological Processnegative regulation of mitotic actomyosin contractile ring contraction
Biological Processpositive regulation of establishment of bipolar cell polarity
Biological Processpositive regulation of establishment or maintenance of bipolar cell polarity regulating cell shape
Biological Processpositive regulation of mitotic cytokinesis, division site positioning
Biological Processpositive regulation of pheromone response MAPK cascade
Biological Processregulation of MAPK cascade
Biological Processregulation of microtubule cytoskeleton organization

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Serine/threonine-protein kinase shk1/pak1
  • EC number

Gene names

    • Name
      shk1
    • Synonyms
      orb2, pak1, ste20
    • ORF names
      SPBC1604.14c

Organism names

Accessions

  • Primary accession
    P50527

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000866511-658Serine/threonine-protein kinase shk1/pak1
Modified residue301Phosphoserine
Modified residue303Phosphoserine

Post-translational modification

Autophosphorylated on serine residues.

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Forms an activated complex with GTP-bound ras-like cdc42. Interacts with skb1 and the SH3 domain of skb5 via its amino-terminal regulatory domain. Skb1, cdc42 and shk1 are able to form a ternary complex in vivo. Interacts with rga8 and may interact with byr2.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region1-21Disordered
Region39-104Disordered
Compositional bias56-96Polar residues
Region126-147Disordered
Domain147-160CRIB
Region213-365Disordered
Compositional bias227-253Polar residues
Compositional bias264-341Polar residues
Domain386-637Protein kinase

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    658
  • Mass (Da)
    72,358
  • Last updated
    2000-05-30 v2
  • Checksum
    69D72E5C925021E5
MERGTLQPRKKAPNGYGITPIVAHKTGEPVRYEVEDDLRKLKPSRTAPKPPAINTNLAEDTFSGFPLSQSRTTVSRVSLGSRQHSSSSIRKLQTNVSDVRSYDERNQKKSAFENFVSSMSSFLTGGGSSPTSSYGSGSASPRKSTVISSPFDPKHVTHVGFNYDTGEFTGMPTEWQALLKVSGITKSEQVQHPQAVLDAMAFYSQSKKYLEEGAKPPFPRESTEKPLLSVSALSSSSHLQPTSATSSSSRLYPSRPAPTPPASSSSSPLLSSQTVKTTTSNASRQPSPLVSSKSTDNIIRSHSPVLLTPQTLSTSETKHIRPNNSTPYQRRAETSTKPKAVATPQKVEAPSAPRLQKRAPRQQSNDSAVLAKLQSICNPKNPTLLYRNFVKIGQGASGDVYSARQVGTNLSVAIKKMNINQQPKKEFIVNEILVMKSHHHKNIVNFIDTFFYKSELWMVMEYMRGGSLTEVVTNNTLSEGQIAAICKETLEGLQHLHENGIVHRDIKSDNILLSLQGDIKLTDFGFCAQIDSNMTKRTTMVGTPYWMAPEVVTRKEYGFKVDVWSLGIMAIEMVEGEPPYLNENPLRALYLIATIGTPKISRPELLSSVFHDFLSKSLTVNPKQRPSSGELLRHPFLKQAVPVSSLIPLIKSIHHSGK

Features

Showing features for compositional bias, sequence conflict.

TypeIDPosition(s)Description
Compositional bias56-96Polar residues
Compositional bias227-253Polar residues
Compositional bias264-341Polar residues
Sequence conflict492-495in Ref. 1; AAC49125
Sequence conflict537in Ref. 1; AAC49125

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U22371
EMBL· GenBank· DDBJ
AAC49125.1
EMBL· GenBank· DDBJ
mRNA
L41552
EMBL· GenBank· DDBJ
AAB52609.1
EMBL· GenBank· DDBJ
Genomic DNA
CU329671
EMBL· GenBank· DDBJ
CAA22347.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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