P50220 · NKX21_MOUSE

  • Protein
    Homeobox protein Nkx-2.1
  • Gene
    Nkx2-1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    5/5

Function

function

Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation (PubMed:22955271).
Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (PubMed:22356123).

Features

Showing features for dna binding.

137250100150200250300350
TypeIDPosition(s)Description
DNA binding161-220Homeobox

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentnucleoplasm
Cellular Componentnucleus
Cellular Componenttranscription regulator complex
Molecular Functioncore promoter sequence-specific DNA binding
Molecular FunctionDNA binding
Molecular FunctionDNA-binding transcription activator activity, RNA polymerase II-specific
Molecular FunctionDNA-binding transcription factor activity
Molecular FunctionDNA-binding transcription factor activity, RNA polymerase II-specific
Molecular Functionenzyme binding
Molecular Functionidentical protein binding
Molecular Functionintronic transcription regulatory region sequence-specific DNA binding
Molecular FunctionRNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular FunctionRNA polymerase II transcription regulatory region sequence-specific DNA binding
Molecular FunctionRNA polymerase II-specific DNA-binding transcription factor binding
Molecular Functionsequence-specific DNA binding
Molecular FunctionTBP-class protein binding
Molecular Functiontranscription cis-regulatory region binding
Molecular Functiontranscription coactivator activity
Molecular Functiontranscription coregulator binding
Biological Processanatomical structure formation involved in morphogenesis
Biological Processanimal organ morphogenesis
Biological Processaxon guidance
Biological Processbrain development
Biological Processcell differentiation
Biological Processcerebral cortex cell migration
Biological Processcerebral cortex GABAergic interneuron differentiation
Biological Processcerebral cortex neuron differentiation
Biological Processclub cell differentiation
Biological Processdevelopmental induction
Biological Processendoderm development
Biological Processepithelial tube branching involved in lung morphogenesis
Biological Processfeeding behavior
Biological Processforebrain dorsal/ventral pattern formation
Biological Processforebrain neuron differentiation
Biological Processforebrain neuron fate commitment
Biological ProcessGABAergic neuron differentiation
Biological Processgene expression
Biological Processglobus pallidus development
Biological Processhippocampus development
Biological Processhypothalamus development
Biological Processinterneuron migration
Biological ProcessLeydig cell differentiation
Biological Processlocomotory behavior
Biological Processlung development
Biological Processlung saccule development
Biological Processnegative regulation of cell migration
Biological Processnegative regulation of DNA-templated transcription
Biological Processnegative regulation of epithelial to mesenchymal transition
Biological Processnegative regulation of transcription by RNA polymerase II
Biological Processnegative regulation of transforming growth factor beta receptor signaling pathway
Biological Processneuron fate commitment
Biological Processneuron migration
Biological Processoligodendrocyte differentiation
Biological Processpattern specification process
Biological Processphospholipid metabolic process
Biological Processpituitary gland development
Biological Processpositive regulation of circadian rhythm
Biological Processpositive regulation of DNA-templated transcription
Biological Processpositive regulation of gene expression
Biological Processpositive regulation of transcription by RNA polymerase II
Biological Processregulation of blood volume by renin-angiotensin
Biological Processregulation of DNA-templated transcription
Biological Processregulation of transcription by RNA polymerase II
Biological Processresponse to hormone
Biological Processresponse to lipopolysaccharide
Biological Processrhythmic process
Biological Processtelencephalon cell migration
Biological Processtelencephalon development
Biological Processthyroid gland development
Biological Processtype II pneumocyte differentiation

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Homeobox protein Nkx-2.1
  • Alternative names
    • Thyroid nuclear factor 1
    • Thyroid transcription factor 1 (TTF-1)
    • Thyroid-specific enhancer-binding protein (T/EBP)

Gene names

    • Name
      Nkx2-1
    • Synonyms
      Nkx-2.1, Titf1, Ttf1

Organism names

  • Taxonomic identifier
  • Strains
    • 129/Sv
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P50220

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000493441-372Homeobox protein Nkx-2.1
Modified residue255Phosphoserine

Post-translational modification

Phosphorylated on serine residues by STK3/MST2.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Thyroid, lung and brain.

Developmental stage

Expressed in lung at least from to 9.5 dpc to adulthood.

Gene expression databases

Interaction

Subunit

Interacts with WWTR1.

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region219-258Disordered
Region269-288Disordered
Region312-340Disordered

Sequence similarities

Belongs to the NK-2 homeobox family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    372
  • Mass (Da)
    38,570
  • Last updated
    1996-10-01 v1
  • Checksum
    377B5CE9B709412F
MSMSPKHTTPFSVSDILSPLEESYKKVGMEGGGLGAPLAAYRQGQAAPPAAAMQQHAVGHHGAVTAAYHMTAAGVPQLSHSAVGGYCNGNLGNMSELPPYQDTMRNSASGPGWYGANPDPRFPAISRFMGPASGMNMSGMGGLGSLGDVSKNMAPLPSAPRRKRRVLFSQAQVYELERRFKQQKYLSAPEREHLASMIHLTPTQVKIWFQNHRYKMKRQAKDKAAQQQLQQDSGGGGGGGGGAGCPQQQQAQQQSPRRVAVPVLVKDGKPCQAGAPAPGAASLQSHAQQQAQQQAQAAQAAAAAISVGSGGAGLGAHPGHQPGSAGQSPDLAHHAASPAGLQGQVSSLSHLNSSGSDYGAMSCSTLLYGRTW

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U19755
EMBL· GenBank· DDBJ
AAA86100.1
EMBL· GenBank· DDBJ
Genomic DNA
BC080868
EMBL· GenBank· DDBJ
AAH80868.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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