P50082 · AMA1_YEAST
- ProteinMeiosis-specific APC/C activator protein AMA1
- GeneAMA1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids593 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). Required for the ubiquitination and subsequent degradation of the B-type cyclin CLB1 by the APC/C complex during meiosis. Required for meiosis I, late meiotic gene expression and spore wall assembly.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | anaphase-promoting complex | |
Molecular Function | anaphase-promoting complex binding | |
Molecular Function | ubiquitin ligase activator activity | |
Biological Process | anaphase-promoting complex-dependent catabolic process | |
Biological Process | ascospore formation | |
Biological Process | ascospore wall assembly | |
Biological Process | cell division | |
Biological Process | meiosis I | |
Biological Process | meiotic cell cycle | |
Biological Process | meiotic chromosome segregation | |
Biological Process | meiotic DNA integrity checkpoint signaling | |
Biological Process | positive regulation of anaphase-promoting complex-dependent catabolic process | |
Biological Process | positive regulation of ascospore-type prospore membrane formation | |
Biological Process | positive regulation of protein catabolic process | |
Biological Process | protein ubiquitination | |
Biological Process | regulation of meiotic cell cycle | |
Biological Process | synaptonemal complex assembly |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMeiosis-specific APC/C activator protein AMA1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP50082
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000050842 | 1-593 | Meiosis-specific APC/C activator protein AMA1 | |||
Sequence: MATPHLYHRYNSKSSNKNINSSGNSTEVDRFIPKSVSRNAYKSIPMLNGFDISYSELCEKSPSPERLSSPEFFNELRNTGHYESISTTNEFSMSSISSSSESQVTRSGSARASRNDYSKLTKEQKDHRKNIAHSLGFQLPDRVFTFETTSAEILEKNKAIKNCFGPGSCAEIRSTFDFSTLSPDVARYYIANSNARSASPQRQIQRPAKRVKSHIPYRVLDAPCLRNDFYSNLISWSRTTNNVLVGLGCSVYIWSEKEGAVSILDHQYLSEKRDLVTCVSFCPYNTYFIVGTKFGRILLYDQKEFFHSSNTNEKEPVFVFQTESFKGICCLEWFKPGEICKFYVGEENGNVSLFEIKSLHFPIKNWSKRQKLEDENLIGLKLHSTYQAQAQQVCGISLNEHANLLAVGGNDNSCSLWDISDLDKPIKKFVLPHKAAVKAIAFCPWSKSLLATGGGSKDRCIKFWHTSTGTLLDEICTSGQVTSLIWSLRHKQIVATFGFGDTKNPVLITLYSYPKLSKLLEVRSPNPLRVLSAVISPSSMAICVATNDETIRFYELWNDKEEIINEIQESGIYGSNIIEYMEGIETTHNKRIR |
Proteomic databases
Expression
Developmental stage
Expressed and spliced only during meiosis dependent on the splicing factor MER1.
Interaction
Subunit
Interacts with CDC16.
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias, motif, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-26 | Disordered | ||||
Sequence: MATPHLYHRYNSKSSNKNINSSGNST | ||||||
Compositional bias | 8-26 | Polar residues | ||||
Sequence: HRYNSKSSNKNINSSGNST | ||||||
Motif | 29-35 | C-box | ||||
Sequence: DRFIPKS | ||||||
Compositional bias | 94-115 | Polar residues | ||||
Sequence: SSISSSSESQVTRSGSARASRN | ||||||
Region | 94-125 | Disordered | ||||
Sequence: SSISSSSESQVTRSGSARASRNDYSKLTKEQK | ||||||
Repeat | 226-264 | WD 1 | ||||
Sequence: RNDFYSNLISWSRTTNNVLVGLGCSVYIWSEKEGAVSIL | ||||||
Repeat | 271-310 | WD 2 | ||||
Sequence: EKRDLVTCVSFCPYNTYFIVGTKFGRILLYDQKEFFHSSN | ||||||
Repeat | 323-364 | WD 3 | ||||
Sequence: ESFKGICCLEWFKPGEICKFYVGEENGNVSLFEIKSLHFPIK | ||||||
Repeat | 388-427 | WD 4 | ||||
Sequence: AQAQQVCGISLNEHANLLAVGGNDNSCSLWDISDLDKPIK | ||||||
Repeat | 432-474 | WD 5 | ||||
Sequence: PHKAAVKAIAFCPWSKSLLATGGGSKDRCIKFWHTSTGTLLDE | ||||||
Repeat | 525-564 | WD 6 | ||||
Sequence: PNPLRVLSAVISPSSMAICVATNDETIRFYELWNDKEEII |
Domain
The C-box is required for the association with the APC/C complex.
Sequence similarities
Belongs to the WD repeat CDC20/Fizzy family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length593
- Mass (Da)67,027
- Last updated2005-01-04 v2
- Checksum35685463E1A677CA
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 8-26 | Polar residues | ||||
Sequence: HRYNSKSSNKNINSSGNST | ||||||
Compositional bias | 94-115 | Polar residues | ||||
Sequence: SSISSSSESQVTRSGSARASRN |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY033394 EMBL· GenBank· DDBJ | AAK61800.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X87941 EMBL· GenBank· DDBJ | CAA61174.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
Z73010 EMBL· GenBank· DDBJ | CAA97253.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
BK006941 EMBL· GenBank· DDBJ | DAA08318.1 EMBL· GenBank· DDBJ | Genomic DNA |