P49866 · HNF4_DROME
- ProteinTranscription factor HNF-4 homolog
- GeneHnf4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids704 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcriptionally controlled transcription factor. Important for the differentiation of various specialized cell types that arise from both endoderm and mesoderm. May have a role in early gut formation. Plays an essential role in lipid catabolism, regulating lipid mobilization and beta-oxidation in response to nutrient deprivation.
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 139-214 | Nuclear receptor | ||||
Sequence: PTVCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHQYTCRFARNCVVDKDKRNQCRYCRLRKCFKAGMKKEAVQN |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | nuclear receptor activity | |
Molecular Function | nuclear steroid receptor activity | |
Molecular Function | RNA polymerase II cis-regulatory region sequence-specific DNA binding | |
Molecular Function | zinc ion binding | |
Biological Process | cell differentiation | |
Biological Process | cellular response to insulin stimulus | |
Biological Process | glucose homeostasis | |
Biological Process | lipid catabolic process | |
Biological Process | lipid oxidation | |
Biological Process | regulation of transcription by RNA polymerase II |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTranscription factor HNF-4 homolog
- Short namesdHNF4
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionP49866
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Under normal culture conditions, the flies progress through development until they die during or shortly after adult eclosion. Under low density culture conditions, many flies survive and develop into morphologically normal adults. The mutant flies are, however, very sensitive to starvation, displaying inability to efficiently mobilize stored lipid in the midgut and fat body, increased levels of long-chain fatty acids and triglycerides, and premature death.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000053565 | 1-704 | Transcription factor HNF-4 homolog | |||
Sequence: MMKHPQDLSVTDDQQLMKVNKVEKMEQELHDPESESHIMHADALASAYPAASQPHSPIGLALSPNGGGLGLSNSSNQSSENFALCNGNGNAGSAGGGSASSGSNNNNSMFSPNNNLSGSGSGTNSSQQQLQQQQQQQSPTVCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHQYTCRFARNCVVDKDKRNQCRYCRLRKCFKAGMKKEAVQNERDRISCRRTSNDDPDPGNGLSVISLVKAENESRQSKAGAAMEPNINEDLSNKQFASINDVCESMKQQLLTLVEWAKQIPAFNELQLDDQVALLRAHAGEHLLLGLSRRSMHLKDVLLLSNNCVITRHCPDPLVSPNLDISRIGARIIDELVTVMKDVGIDDTEFACIKALVFFDPNAKGLNEPHRIKSLRHQILNNLEDYISDRQYESRGRFGEILLILPVLQSITWQMIEQIQFAKIFGVAHIDSLLQEMLLGGELADNPLPLSPPNQSNDYQSPTHTGNMEGGNQVNSSLDSLATSGGPGSHSLDLEVQHIQALIEANSADDSFRAYAASTAAAAAAAVSSSSSAPASVAPASISPPLNSPKSQHQHQQHATHQQQQESSYLDMPVKHYNGSRSGPLPTQHSPQRMHPYQRAVASPVEVSSGGGGLGLRNPADITLNEYNRSEGSSAEELLRRTPLKIRAPEMLTAPAGYGTEPCRMTLKQEPETGY |
Proteomic databases
Expression
Tissue specificity
In third instar larvae, expressed at high levels in midgut and attached gastric caeca, fat body, Malpighian tubules and oenocytes, and at lower levels in proventriculus, salivary glands, epidermis, brain and ring gland. Not detected in imaginal disks and the median neurosecretory cells that produce insulin-like peptides (at protein level). In developing embryos, expressed in mid-gut, fat bodies and the distal region of Malpighian tubules.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, zinc finger, domain, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 93-133 | Disordered | ||||
Sequence: SAGGGSASSGSNNNNSMFSPNNNLSGSGSGTNSSQQQLQQQ | ||||||
Zinc finger | 142-162 | NR C4-type | ||||
Sequence: CAICGDRATGKHYGASSCDGC | ||||||
Zinc finger | 178-197 | NR C4-type | ||||
Sequence: CRFARNCVVDKDKRNQCRYC | ||||||
Domain | 232-470 | NR LBD | ||||
Sequence: GNGLSVISLVKAENESRQSKAGAAMEPNINEDLSNKQFASINDVCESMKQQLLTLVEWAKQIPAFNELQLDDQVALLRAHAGEHLLLGLSRRSMHLKDVLLLSNNCVITRHCPDPLVSPNLDISRIGARIIDELVTVMKDVGIDDTEFACIKALVFFDPNAKGLNEPHRIKSLRHQILNNLEDYISDRQYESRGRFGEILLILPVLQSITWQMIEQIQFAKIFGVAHIDSLLQEMLLGG | ||||||
Region | 474-520 | Disordered | ||||
Sequence: DNPLPLSPPNQSNDYQSPTHTGNMEGGNQVNSSLDSLATSGGPGSHS | ||||||
Compositional bias | 478-516 | Polar residues | ||||
Sequence: PLSPPNQSNDYQSPTHTGNMEGGNQVNSSLDSLATSGGP | ||||||
Compositional bias | 563-600 | Polar residues | ||||
Sequence: PASVAPASISPPLNSPKSQHQHQQHATHQQQQESSYLD | ||||||
Region | 563-624 | Disordered | ||||
Sequence: PASVAPASISPPLNSPKSQHQHQQHATHQQQQESSYLDMPVKHYNGSRSGPLPTQHSPQRMH | ||||||
Region | 684-704 | Disordered | ||||
Sequence: PAGYGTEPCRMTLKQEPETGY |
Sequence similarities
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing.
P49866-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameA
- Length704
- Mass (Da)76,388
- Last updated2014-05-14 v3
- Checksum5868280AFE7A00CC
P49866-2
- NameD
- Differences from canonical
- 1-32: MMKHPQDLSVTDDQQLMKVNKVEKMEQELHDP → MYASMLPSLLNMKTENLTSSSYDDAFLLEENLLHIM
P49866-3
- NameB
- Differences from canonical
- 1-32: MMKHPQDLSVTDDQQLMKVNKVEKMEQELHDP → MVRKSGRVKISSRDRVAVGNILLRGKVGGGRVAVAAAEEAEAGRRRRRRDSSASRTASSD
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
X2J9X1 | X2J9X1_DROME | Hnf4 | 666 |
Sequence caution
Features
Showing features for alternative sequence, sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_054578 | 1-32 | in isoform B | |||
Sequence: MMKHPQDLSVTDDQQLMKVNKVEKMEQELHDP → MVRKSGRVKISSRDRVAVGNILLRGKVGGGRVAVAAAEEAEAGRRRRRRDSSASRTASSD | ||||||
Alternative sequence | VSP_054577 | 1-32 | in isoform D | |||
Sequence: MMKHPQDLSVTDDQQLMKVNKVEKMEQELHDP → MYASMLPSLLNMKTENLTSSSYDDAFLLEENLLHIM | ||||||
Sequence conflict | 113 | in Ref. 1; AAB09592 | ||||
Sequence: N → Y | ||||||
Sequence conflict | 117 | in Ref. 1; AAB09592 | ||||
Sequence: S → N | ||||||
Sequence conflict | 159 | in Ref. 4; AAN71555 | ||||
Sequence: C → F | ||||||
Compositional bias | 478-516 | Polar residues | ||||
Sequence: PLSPPNQSNDYQSPTHTGNMEGGNQVNSSLDSLATSGGP | ||||||
Compositional bias | 563-600 | Polar residues | ||||
Sequence: PASVAPASISPPLNSPKSQHQHQQHATHQQQQESSYLD |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U70874 EMBL· GenBank· DDBJ | AAB09592.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AE014134 EMBL· GenBank· DDBJ | AAF52702.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014134 EMBL· GenBank· DDBJ | AAF52703.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014134 EMBL· GenBank· DDBJ | AAN10680.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014134 EMBL· GenBank· DDBJ | ABV53653.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT001800 EMBL· GenBank· DDBJ | AAN71555.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
BT003533 EMBL· GenBank· DDBJ | AAO39537.1 EMBL· GenBank· DDBJ | mRNA | ||
BT088848 EMBL· GenBank· DDBJ | ACS68165.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |