P49866 · HNF4_DROME

Function

function

Transcriptionally controlled transcription factor. Important for the differentiation of various specialized cell types that arise from both endoderm and mesoderm. May have a role in early gut formation. Plays an essential role in lipid catabolism, regulating lipid mobilization and beta-oxidation in response to nutrient deprivation.

Features

Showing features for dna binding.

170450100150200250300350400450500550600650700
TypeIDPosition(s)Description
DNA binding139-214Nuclear receptor

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentnucleus
Molecular Functionnuclear receptor activity
Molecular Functionnuclear steroid receptor activity
Molecular FunctionRNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Functionzinc ion binding
Biological Processcell differentiation
Biological Processcellular response to insulin stimulus
Biological Processglucose homeostasis
Biological Processlipid catabolic process
Biological Processlipid oxidation
Biological Processregulation of transcription by RNA polymerase II

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Transcription factor HNF-4 homolog
  • Short names
    dHNF4
  • Alternative names
    • Nuclear receptor subfamily 2 group A member 4

Gene names

    • Name
      Hnf4
    • Synonyms
      NR2A4
    • ORF names
      CG9310

Organism names

  • Taxonomic identifier
  • Strain
    • Berkeley
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora

Accessions

  • Primary accession
    P49866
  • Secondary accessions
    • A8DYX8
    • C5WLN3
    • Q8IGG2
    • Q8IPF2
    • Q9VLI7
    • Q9VLI8

Proteomes

Organism-specific databases

Subcellular Location

Nucleus

Keywords

Phenotypes & Variants

Disruption phenotype

Under normal culture conditions, the flies progress through development until they die during or shortly after adult eclosion. Under low density culture conditions, many flies survive and develop into morphologically normal adults. The mutant flies are, however, very sensitive to starvation, displaying inability to efficiently mobilize stored lipid in the midgut and fat body, increased levels of long-chain fatty acids and triglycerides, and premature death.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000535651-704Transcription factor HNF-4 homolog

Proteomic databases

Expression

Tissue specificity

In third instar larvae, expressed at high levels in midgut and attached gastric caeca, fat body, Malpighian tubules and oenocytes, and at lower levels in proventriculus, salivary glands, epidermis, brain and ring gland. Not detected in imaginal disks and the median neurosecretory cells that produce insulin-like peptides (at protein level). In developing embryos, expressed in mid-gut, fat bodies and the distal region of Malpighian tubules.

Gene expression databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, zinc finger, domain, compositional bias.

TypeIDPosition(s)Description
Region93-133Disordered
Zinc finger142-162NR C4-type
Zinc finger178-197NR C4-type
Domain232-470NR LBD
Region474-520Disordered
Compositional bias478-516Polar residues
Compositional bias563-600Polar residues
Region563-624Disordered
Region684-704Disordered

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete

This entry describes 3 isoforms produced by Alternative splicing.

P49866-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    704
  • Mass (Da)
    76,388
  • Last updated
    2014-05-14 v3
  • Checksum
    5868280AFE7A00CC
MMKHPQDLSVTDDQQLMKVNKVEKMEQELHDPESESHIMHADALASAYPAASQPHSPIGLALSPNGGGLGLSNSSNQSSENFALCNGNGNAGSAGGGSASSGSNNNNSMFSPNNNLSGSGSGTNSSQQQLQQQQQQQSPTVCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHQYTCRFARNCVVDKDKRNQCRYCRLRKCFKAGMKKEAVQNERDRISCRRTSNDDPDPGNGLSVISLVKAENESRQSKAGAAMEPNINEDLSNKQFASINDVCESMKQQLLTLVEWAKQIPAFNELQLDDQVALLRAHAGEHLLLGLSRRSMHLKDVLLLSNNCVITRHCPDPLVSPNLDISRIGARIIDELVTVMKDVGIDDTEFACIKALVFFDPNAKGLNEPHRIKSLRHQILNNLEDYISDRQYESRGRFGEILLILPVLQSITWQMIEQIQFAKIFGVAHIDSLLQEMLLGGELADNPLPLSPPNQSNDYQSPTHTGNMEGGNQVNSSLDSLATSGGPGSHSLDLEVQHIQALIEANSADDSFRAYAASTAAAAAAAVSSSSSAPASVAPASISPPLNSPKSQHQHQQHATHQQQQESSYLDMPVKHYNGSRSGPLPTQHSPQRMHPYQRAVASPVEVSSGGGGLGLRNPADITLNEYNRSEGSSAEELLRRTPLKIRAPEMLTAPAGYGTEPCRMTLKQEPETGY

P49866-2

  • Name
    D
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-32: MMKHPQDLSVTDDQQLMKVNKVEKMEQELHDP → MYASMLPSLLNMKTENLTSSSYDDAFLLEENLLHIM

P49866-3

  • Name
    B
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-32: MMKHPQDLSVTDDQQLMKVNKVEKMEQELHDP → MVRKSGRVKISSRDRVAVGNILLRGKVGGGRVAVAAAEEAEAGRRRRRRDSSASRTASSD

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
X2J9X1X2J9X1_DROMEHnf4666

Sequence caution

The sequence AAB09592.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.
The sequence AAN71555.1 differs from that shown. Reason: Erroneous initiation Extended N-terminus.
The sequence ACS68165.1 differs from that shown. Reason: Erroneous initiation Extended N-terminus.

Features

Showing features for alternative sequence, sequence conflict, compositional bias.

TypeIDPosition(s)Description
Alternative sequenceVSP_0545781-32in isoform B
Alternative sequenceVSP_0545771-32in isoform D
Sequence conflict113in Ref. 1; AAB09592
Sequence conflict117in Ref. 1; AAB09592
Sequence conflict159in Ref. 4; AAN71555
Compositional bias478-516Polar residues
Compositional bias563-600Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U70874
EMBL· GenBank· DDBJ
AAB09592.1
EMBL· GenBank· DDBJ
mRNA Different initiation
AE014134
EMBL· GenBank· DDBJ
AAF52702.2
EMBL· GenBank· DDBJ
Genomic DNA
AE014134
EMBL· GenBank· DDBJ
AAF52703.2
EMBL· GenBank· DDBJ
Genomic DNA
AE014134
EMBL· GenBank· DDBJ
AAN10680.1
EMBL· GenBank· DDBJ
Genomic DNA
AE014134
EMBL· GenBank· DDBJ
ABV53653.1
EMBL· GenBank· DDBJ
Genomic DNA
BT001800
EMBL· GenBank· DDBJ
AAN71555.1
EMBL· GenBank· DDBJ
mRNA Different initiation
BT003533
EMBL· GenBank· DDBJ
AAO39537.1
EMBL· GenBank· DDBJ
mRNA
BT088848
EMBL· GenBank· DDBJ
ACS68165.1
EMBL· GenBank· DDBJ
mRNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp