P49723 · RIR4_YEAST
- ProteinRibonucleoside-diphosphate reductase small chain 2
- GeneRNR4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids345 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. RNR4 is required for proper folding of RNR2 and assembly with the large subunits.
Miscellaneous
Present with 88884 molecules/cell in log phase SD medium.
Lacks 3 iron-binding residues conserved in all other R2 subunits.
Catalytic activity
- [thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate
RHEA-COMP:10700 CHEBI:50058 Position: n/n+3+ CHEBI:73316 + CHEBI:15377 = RHEA-COMP:10698 CHEBI:29950 Position: nCHEBI:29950 Position: n+3+ CHEBI:57930
Kinetics
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
2250 nmol/min/mg | for cytidine 5'-diphosphate |
Temperature Dependence
Optimum temperature is 30 degrees Celsius.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 131 | |||||
Sequence: Y |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Cellular Component | ribonucleoside-diphosphate reductase complex | |
Molecular Function | protein heterodimerization activity | |
Molecular Function | ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor | |
Biological Process | deoxyribonucleotide biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibonucleoside-diphosphate reductase small chain 2
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP49723
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Found predominantly in the nucleus under normal growth conditions and is redistributed to the cytoplasm in damaged cells in a DNA replication and damage checkpoint-dependent manner. Nuclear localization is mediated by DIF1.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for modified residue, chain, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 1 | N-acetylmethionine | ||||
Sequence: M | ||||||
Chain | PRO_0000190465 | 1-345 | Ribonucleoside-diphosphate reductase small chain 2 | |||
Sequence: MEAHNQFLKTFQKERHDMKEAEKDEILLMENSRRFVMFPIKYHEIWAAYKKVEASFWTAEEIELAKDTEDFQKLTDDQKTYIGNLLALSISSDNLVNKYLIENFSAQLQNPEGKSFYGFQIMMENIYSEVYSMMVDAFFKDPKNIPLFKEIANLPEVKHKAAFIERWISNDDSLYAERLVAFAAKEGIFQAGNYASMFWLTDKKIMPGLAMANRNICRDRGAYTDFSCLLFAHLRTKPNPKIIEKIITEAVEIEKEYYSNSLPVEKFGMDLKSIHTYIEFVADGLLQGFGNEKYYNAVNPFEFMEDVATAGKTTFFEKKVSDYQKASDMSKSATPSKEINFDDDF | ||||||
Modified residue | 169 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 332 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 334 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 336 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 337 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Induction
Induced by DNA-damage.
Interaction
Subunit
Heterotetramer of two large (R1) and two small (R2) subunits. S.cerevisiae has two different R1 subunits (RNR1 and RNR3) and two different R2 subunits (RNR2 and RNR4). The functional form of the small subunits is a RNR2-RNR4 heterodimer, where RNR2 provides the iron-radical center and RNR4 is required for proper folding of RNR2 and assembly with the large subunits. Under normal growth conditions, the active form of the large subunits is a homodimer of the constitutively expressed RNR1. In damaged cells or cells arrested for DNA synthesis, the reductase consists of multiple species because of the association of the small subunits (RNR2-RNR4) with either the RNR1 homodimer or a heterodimer of RNR1 and the damage-inducible RNR3. Interacts with DIF1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P49723 | RNR1 P21524 | 6 | EBI-15251, EBI-15234 | |
BINARY | P49723 | RNR2 P09938 | 9 | EBI-15251, EBI-15240 | |
BINARY | P49723 | WTM1 Q12363 | 6 | EBI-15251, EBI-20563 | |
BINARY | P49723 | WTM2 Q12206 | 3 | EBI-15251, EBI-20571 |
Protein-protein interaction databases
Miscellaneous
Structure
Sequence
- Sequence statusComplete
- Length345
- Mass (Da)40,055
- Last updated1996-10-01 v1
- Checksum712853F2D87D3972
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 127 | in Ref. 4; AAU09736 | ||||
Sequence: Y → H |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U30385 EMBL· GenBank· DDBJ | AAB72236.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z72965 EMBL· GenBank· DDBJ | CAA97206.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY723819 EMBL· GenBank· DDBJ | AAU09736.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006941 EMBL· GenBank· DDBJ | DAA08275.1 EMBL· GenBank· DDBJ | Genomic DNA |