P49641 · MA2A2_HUMAN
- ProteinAlpha-mannosidase 2x
- GeneMAN2A2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1150 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
Catalytic activity
- 2 H2O + N4-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] = 2 alpha-D-mannopyranose + N4-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein]
Cofactor
Note: Binds 1 zinc ion per subunit.
Pathway
Protein modification; protein glycosylation.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Golgi membrane | |
Molecular Function | alpha-mannosidase activity | |
Molecular Function | carbohydrate binding | |
Molecular Function | hydrolase activity, hydrolyzing N-glycosyl compounds | |
Molecular Function | mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity | |
Molecular Function | metal ion binding | |
Biological Process | mannose metabolic process | |
Biological Process | N-glycan processing | |
Biological Process | protein glycosylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameAlpha-mannosidase 2x
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionP49641
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-5 | Cytoplasmic | ||||
Sequence: MKLKK | ||||||
Transmembrane | 6-26 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: QVTVCGAAIFCVAVFSLYLML | ||||||
Topological domain | 27-796 | Lumenal | ||||
Sequence: DRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPAMLPYYTVNGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTVSEELPFDNVDGGVWRQGFDISYDPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNINVQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLERNLGATPRSGWAVDPFGYSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSQLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFFNSRPNLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEVLYSLAAAHARRSGLAGRYPLSDFTLLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVNLKQVIIHAAHYLVLGDKETYHFDPEAPFLQVDDTRLSHDALPERTVIQLDSSPRFVVLFNPLEQERFSMVSLLVNSPRVRVLSEEGQPLAVQISAHWSSATEAVPDVYQVSVPVRLPALGLGVLQLQLGLDGHRTLPSSVRIYLHGRQLSVSRHEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEHEQQVD |
Keywords
- Cellular component
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_047912 | 412 | in dbSNP:rs2106673 | |||
Sequence: Q → R | ||||||
Natural variant | VAR_047913 | 665 | in dbSNP:rs1266494 | |||
Sequence: S → F |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 1,402 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000206905 | 1-1150 | Alpha-mannosidase 2x | |||
Sequence: MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPAMLPYYTVNGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTVSEELPFDNVDGGVWRQGFDISYDPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNINVQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLERNLGATPRSGWAVDPFGYSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSQLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFFNSRPNLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEVLYSLAAAHARRSGLAGRYPLSDFTLLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVNLKQVIIHAAHYLVLGDKETYHFDPEAPFLQVDDTRLSHDALPERTVIQLDSSPRFVVLFNPLEQERFSMVSLLVNSPRVRVLSEEGQPLAVQISAHWSSATEAVPDVYQVSVPVRLPALGLGVLQLQLGLDGHRTLPSSVRIYLHGRQLSVSRHEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEHEQQVDMQVLVYGTRTSKDKSGAYLFLPDGEAKPYVPKEPPVLRVTEGPFFSEVVAYYEHIHQAVRLYNLPGVEGLSLDISSLVDIRDYVNKELALHIHTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQKRLTLHTAQALGVSSLKDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNRFRLLLERRTVGSEVQDSHSTSYPSLLSHLTSMYLNAPALALPVARMQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEEDTLPSAETALILHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYLEPMEIATFRLRLG | ||||||
Glycosylation | 95 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 305 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 1093 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 1131 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Interaction
Subunit
Homodimer; disulfide-linked (By similarity).
Interacts with MGAT4D (By similarity).
Interacts with MGAT4D (By similarity).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P49641 | B4GALT6 Q9UBX8 | 2 | EBI-713521, EBI-21514152 |
Protein-protein interaction databases
Miscellaneous
Structure
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing. Additional isoforms seem to exist.
P49641-3
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name3
- Length1,150
- Mass (Da)130,539
- Last updated2011-09-21 v3
- ChecksumBFB3D763A6A55444
P49641-1
- Name1
- SynonymsLong
P49641-2
- Name2
- SynonymsShort
- NoteMay be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
- Differences from canonical
- 797-1150: Missing
Computationally mapped potential isoform sequences
There are 14 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
H0YMU0 | H0YMU0_HUMAN | MAN2A2 | 72 | ||
H0YML7 | H0YML7_HUMAN | MAN2A2 | 58 | ||
H0YNG5 | H0YNG5_HUMAN | MAN2A2 | 938 | ||
H0YNC8 | H0YNC8_HUMAN | MAN2A2 | 76 | ||
H0YLU3 | H0YLU3_HUMAN | MAN2A2 | 319 | ||
H0YLL4 | H0YLL4_HUMAN | MAN2A2 | 164 | ||
H0YL67 | H0YL67_HUMAN | MAN2A2 | 120 | ||
H0YKM7 | H0YKM7_HUMAN | MAN2A2 | 329 | ||
H0YKQ2 | H0YKQ2_HUMAN | MAN2A2 | 195 | ||
H0YKT0 | H0YKT0_HUMAN | MAN2A2 | 264 | ||
H0YKH9 | H0YKH9_HUMAN | MAN2A2 | 360 | ||
H0YLB9 | H0YLB9_HUMAN | MAN2A2 | 793 | ||
H0YK77 | H0YK77_HUMAN | MAN2A2 | 50 | ||
A0A0C4DGL1 | A0A0C4DGL1_HUMAN | MAN2A2 | 796 |
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_041732 | 783-796 | in isoform 1 | |||
Sequence: SIRRVDEEHEQQVD → GSGLCFLAEHPKGG | ||||||
Alternative sequence | VSP_041733 | 797-1150 | in isoform 2 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_041734 | 823-906 | in isoform 1 | |||
Sequence: KPYVPKEPPVLRVTEGPFFSEVVAYYEHIHQAVRLYNLPGVEGLSLDISSLVDIRDYVNKELALHIHTDIDSQGIFFTDLNGFQ → SPTSPRSPPCCVSLKALSSQRWLRTMSTFTRRSGFTICQGWRGCLWTYHPWWTSGTTSTRSWPCTSIQTSTAR |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D55649 EMBL· GenBank· DDBJ | BAA09510.1 EMBL· GenBank· DDBJ | mRNA | ||
L28821 EMBL· GenBank· DDBJ | AAA92022.1 EMBL· GenBank· DDBJ | mRNA | ||
AK289863 EMBL· GenBank· DDBJ | BAF82552.1 EMBL· GenBank· DDBJ | mRNA | ||
AC067986 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AC068831 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC136448 EMBL· GenBank· DDBJ | AAI36449.1 EMBL· GenBank· DDBJ | mRNA |