P49598 · P2C37_ARATH

Function

function

Major negative regulator of abscisic acid (ABA) responses during seed germination and cold acclimation. Confers insensitivity to ABA. Modulates negatively the AKT2/3 activity, which mediates K+ transport and membrane polarization during stress situations, probably by dephosphorylation. Prevents stomata closure by inactivating the S-type anion efflux channel SLAC1 and its activator SRK2E. Represses KIN10 activity by the specific dephosphorylation of its T-loop Thr-198, leading to a poststress inactivation of SnRK1 signaling (PubMed:24179127).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 magnesium or manganese ions per subunit.

Activity regulation

Repressed by PYR/PYL/RCAR ABA receptors in an ABA-dependent manner.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site142Mn2+ 1 (UniProtKB | ChEBI)
Binding site142Mn2+ 2 (UniProtKB | ChEBI)
Binding site143Mn2+ 1 (UniProtKB | ChEBI)
Binding site146Zn2+ (UniProtKB | ChEBI)
Binding site148Zn2+ (UniProtKB | ChEBI)
Binding site208Zn2+ (UniProtKB | ChEBI)
Binding site210Zn2+ (UniProtKB | ChEBI)
Site280Lock
Binding site327Mn2+ 1 (UniProtKB | ChEBI)
Binding site327Mn2+ 2 (UniProtKB | ChEBI)
Binding site331Mn2+ 1 (UniProtKB | ChEBI)
Binding site380Mn2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentnucleus
Molecular Functionkinase binding
Molecular Functionmetal ion binding
Molecular Functionmyosin phosphatase activity
Molecular Functionphosphatase activity
Molecular Functionphosphoprotein phosphatase activity
Molecular Functionprotein kinase binding
Molecular Functionprotein serine/threonine phosphatase activity
Biological Processabscisic acid-activated signaling pathway
Biological Processdefense response to virus
Biological Processnegative regulation of abscisic acid-activated signaling pathway
Biological Processnegative regulation of anion channel activity
Biological Processregulation of stomatal movement
Biological Processresponse to abscisic acid
Biological Processresponse to cold
Biological Processresponse to water deprivation

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Protein phosphatase 2C 37
  • EC number
  • Short names
    AtPP2C37
  • Alternative names
    • Protein ABA-HYPERSENSITIVE GERMINATION 3
    • Protein phosphatase 2C A (PP2CA)

Gene names

    • Name
      PP2CA
    • Synonyms
      AHG3
    • ORF names
      F24K9.8
    • Ordered locus names
      At3g11410

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    P49598

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis139Loss of phosphatase activity. Loss of kinase activity but intact binding and repression of SRK2E; when associated with D-145.
Mutagenesis145Insensitive to ABA and loss of phosphatase activity. Loss of kinase activity but intact binding and repression of SRK2E; when associated with D-139.
Mutagenesis287In ahg3-1; hypersensitivity to ABA during seed germination, and loss of phosphatase activity.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 49 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000577691-399Protein phosphatase 2C 37

Post-translational modification

Ubiquitinated by RGLG1 and RGLG5 in response to abscisic acid (ABA). Ubiquitination of PP2CA leads to its degradation by the proteasome.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Mostly expressed in seeds and leaves, and, to a lower extent, in roots, stems, and flowers, particularly in siliques. Essentially found in the phloem.

Induction

Repressed by MYB44. Induced by cold stress, drought, high salt, and ABA.

Gene expression databases

Interaction

Subunit

Interacts with AKT2/AKT3. Interacts with ABA-bounded PYR1, PYL1, PYL2, PYL3, PYL4, PYL9 and PYL12, and with free PYL2, PYL3, PYL4 and PYL13. Binds to and inactivates SLAC1 and SRK2E. The inactivation of SRK2E does not require phosphatase activity. Interacts with CBL1, CBL2, CBL3, CBL5, and CBL7, but not CBL4, CBL6, and CBL9 (PubMed:11181729, PubMed:12034902, PubMed:19407142, PubMed:19955427, PubMed:21596690, PubMed:24165892).
Interacts with RGLG1 and RGLG5 (PubMed:27577789).
Interacts with KIN10 (PubMed:24179127).

Binary interactions

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain104-389PPM-type phosphatase

Domain

The 'lock' site stabilizes the complex made of PP2C, ABA and PYR/PYL/RCAR receptor by keeping receptor 'gate' and 'latch' loops in closed positions.

Sequence similarities

Belongs to the PP2C family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    399
  • Mass (Da)
    43,350
  • Last updated
    1996-02-01 v1
  • Checksum
    83B82E32FEC71D4D
MAGICCGVVGETEPAAPVDSTSRASLRRRLDLLPSIKIVADSAVAPPLENCRKRQKRETVVLSTLPGNLDLDSNVRSENKKARSAVTNSNSVTEAESFFSDVPKIGTTSVCGRRRDMEDAVSIHPSFLQRNSENHHFYGVFDGHGCSHVAEKCRERLHDIVKKEVEVMASDEWTETMVKSFQKMDKEVSQRECNLVVNGATRSMKNSCRCELQSPQCDAVGSTAVVSVVTPEKIIVSNCGDSRAVLCRNGVAIPLSVDHKPDRPDELIRIQQAGGRVIYWDGARVLGVLAMSRAIGDNYLKPYVIPDPEVTVTDRTDEDECLILASDGLWDVVPNETACGVARMCLRGAGAGDDSDAAHNACSDAALLLTKLALARQSSDNVSVVVVDLRKRRNNQASS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D38109
EMBL· GenBank· DDBJ
BAA07287.1
EMBL· GenBank· DDBJ
mRNA
AC008153
EMBL· GenBank· DDBJ
AAG51448.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002686
EMBL· GenBank· DDBJ
AEE75044.1
EMBL· GenBank· DDBJ
Genomic DNA
AY074368
EMBL· GenBank· DDBJ
AAL67064.1
EMBL· GenBank· DDBJ
mRNA
AY091391
EMBL· GenBank· DDBJ
AAM14330.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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