P49279 · NRAM1_HUMAN
- ProteinNatural resistance-associated macrophage protein 1
- GeneSLC11A1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids550 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Macrophage-specific antiporter that fluxes metal ions in either direction against a proton gradient. Localized to late endosomal lysosomal membranes, delivers bivalent cations from the cytosol into these acidic compartments where they may directly affect antimicrobial activity (PubMed:11237855).
Involved in iron metabolism and host natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe2+ and Mn2+, cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes (Probable).
Involved in iron metabolism and host natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe2+ and Mn2+, cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes (Probable).
Catalytic activity
- H+(out) + Zn2+(in) = H+(in) + Zn2+(out)
- Fe2+(in) + H+(out) = Fe2+(out) + H+(in)
- H+(out) + Mn2+(in) = H+(in) + Mn2+(out)
GO annotations
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameNatural resistance-associated macrophage protein 1
- Short namesNRAMP 1
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionP49279
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Late endosome membrane ; Multi-pass membrane protein
Lysosome membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-58 | Cytoplasmic | ||||
Sequence: MTGDKGPQRLSGSSYGSISSPTSPTSPGPQQAPPRETYLSEKIPIPDTKPGTFSLRKL | ||||||
Transmembrane | 59-76 | Helical | ||||
Sequence: WAFTGPGFLMSIAFLDPG | ||||||
Topological domain | 77-85 | Extracellular | ||||
Sequence: NIESDLQAG | ||||||
Transmembrane | 86-105 | Helical | ||||
Sequence: AVAGFKLLWVLLWATVLGLL | ||||||
Topological domain | 106-142 | Cytoplasmic | ||||
Sequence: CQRLAARLGVVTGKDLGEVCHLYYPKVPRTVLWLTIE | ||||||
Transmembrane | 143-163 | Helical | ||||
Sequence: LAIVGSDMQEVIGTAIAFNLL | ||||||
Topological domain | 164-167 | Extracellular | ||||
Sequence: SAGR | ||||||
Transmembrane | 168-187 | Helical | ||||
Sequence: IPLWGGVLITIVDTFFFLFL | ||||||
Topological domain | 188-196 | Cytoplasmic | ||||
Sequence: DNYGLRKLE | ||||||
Transmembrane | 197-217 | Helical | ||||
Sequence: AFFGLLITIMALTFGYEYVVA | ||||||
Topological domain | 218-240 | Extracellular | ||||
Sequence: RPEQGALLRGLFLPSCPGCGHPE | ||||||
Transmembrane | 241-259 | Helical | ||||
Sequence: LLQAVGIVGAIIMPHNIYL | ||||||
Topological domain | 260-287 | Cytoplasmic | ||||
Sequence: HSALVKSREIDRARRADIREANMYFLIE | ||||||
Transmembrane | 288-307 | Helical | ||||
Sequence: ATIALSVSFIINLFVMAVFG | ||||||
Topological domain | 308-349 | Extracellular | ||||
Sequence: QAFYQKTNQAAFNICANSSLHDYAKIFPMNNATVAVDIYQGG | ||||||
Transmembrane | 350-369 | Helical | ||||
Sequence: VILGCLFGPAALYIWAIGLL | ||||||
Topological domain | 370-400 | Cytoplasmic | ||||
Sequence: AAGQSSTMTGTYAGQFVMEGFLRLRWSRFAR | ||||||
Transmembrane | 401-418 | Helical | ||||
Sequence: VLLTRSCAILPTVLVAVF | ||||||
Topological domain | 419-429 | Extracellular | ||||
Sequence: RDLRDLSGLND | ||||||
Transmembrane | 430-450 | Helical | ||||
Sequence: LLNVLQSLLLPFAVLPILTFT | ||||||
Topological domain | 451-466 | Cytoplasmic | ||||
Sequence: SMPTLMQEFANGLLNK | ||||||
Transmembrane | 467-488 | Helical | ||||
Sequence: VVTSSIMVLVCAINLYFVVSYL | ||||||
Topological domain | 489-496 | Extracellular | ||||
Sequence: PSLPHPAY | ||||||
Transmembrane | 497-516 | Helical | ||||
Sequence: FGLAALLAAAYLGLSTYLVW | ||||||
Topological domain | 517-550 | Cytoplasmic | ||||
Sequence: TCCLAHGATFLAHSSHHHFLYGLLEEDQKGETSG |
Keywords
- Cellular component
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_004629 | 30 | in dbSNP:rs751872662 | |||
Sequence: Q → R | ||||||
Natural variant | VAR_004630 | 318 | in dbSNP:rs201565523 | |||
Sequence: A → V | ||||||
Natural variant | VAR_004631 | 543 | risk factor for infection with Mycobacterium ulcerans; dbSNP:rs17235409 | |||
Sequence: D → N |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 676 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000212588 | 1-550 | Natural resistance-associated macrophage protein 1 | |||
Sequence: MTGDKGPQRLSGSSYGSISSPTSPTSPGPQQAPPRETYLSEKIPIPDTKPGTFSLRKLWAFTGPGFLMSIAFLDPGNIESDLQAGAVAGFKLLWVLLWATVLGLLCQRLAARLGVVTGKDLGEVCHLYYPKVPRTVLWLTIELAIVGSDMQEVIGTAIAFNLLSAGRIPLWGGVLITIVDTFFFLFLDNYGLRKLEAFFGLLITIMALTFGYEYVVARPEQGALLRGLFLPSCPGCGHPELLQAVGIVGAIIMPHNIYLHSALVKSREIDRARRADIREANMYFLIEATIALSVSFIINLFVMAVFGQAFYQKTNQAAFNICANSSLHDYAKIFPMNNATVAVDIYQGGVILGCLFGPAALYIWAIGLLAAGQSSTMTGTYAGQFVMEGFLRLRWSRFARVLLTRSCAILPTVLVAVFRDLRDLSGLNDLLNVLQSLLLPFAVLPILTFTSMPTLMQEFANGLLNKVVTSSIMVLVCAINLYFVVSYLPSLPHPAYFGLAALLAAAYLGLSTYLVWTCCLAHGATFLAHSSHHHFLYGLLEEDQKGETSG | ||||||
Glycosylation | 324 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 338 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Macrophages; peripheral blood leukocytes, lung, spleen and liver.
Induction
In response to lymphokine or bacterial products.
Gene expression databases
Organism-specific databases
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-25 | Polar residues | ||||
Sequence: MTGDKGPQRLSGSSYGSISSPTSPT | ||||||
Region | 1-45 | Disordered | ||||
Sequence: MTGDKGPQRLSGSSYGSISSPTSPTSPGPQQAPPRETYLSEKIPI |
Sequence similarities
Belongs to the NRAMP family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
P49279-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length550
- Mass (Da)59,872
- Last updated1996-02-01 v1
- ChecksumAA670763D52EEECC
P49279-2
- Name2
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q9HBK0 | Q9HBK0_HUMAN | SLC11A1 | 163 | ||
A0A0A0MTL3 | A0A0A0MTL3_HUMAN | SLC11A1 | 82 | ||
A0A087X181 | A0A087X181_HUMAN | SLC11A1 | 41 | ||
A0A087X2D6 | A0A087X2D6_HUMAN | SLC11A1 | 90 |
Features
Showing features for alternative sequence, compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_047875 | 1-13 | in isoform 2 | |||
Sequence: MTGDKGPQRLSGS → MVRCQLTATSTSQ | ||||||
Compositional bias | 1-25 | Polar residues | ||||
Sequence: MTGDKGPQRLSGSSYGSISSPTSPT | ||||||
Alternative sequence | VSP_047876 | 14-131 | in isoform 2 | |||
Sequence: Missing | ||||||
Sequence conflict | 54 | in Ref. 8; CAA57541 | ||||
Sequence: S → R | ||||||
Sequence conflict | 84 | in Ref. 8 | ||||
Sequence: A → L | ||||||
Sequence conflict | 86 | in Ref. 8 | ||||
Sequence: A → P | ||||||
Sequence conflict | 275 | in Ref. 8; CAA57541 | ||||
Sequence: A → V | ||||||
Sequence conflict | 305 | in Ref. 8; CAA57541 | ||||
Sequence: V → A | ||||||
Sequence conflict | 315 | in Ref. 8; CAA57541 | ||||
Sequence: N → K | ||||||
Sequence conflict | 397 | in Ref. 8; CAA57541 | ||||
Sequence: R → S | ||||||
Sequence conflict | 441 | in Ref. 8; CAA57541 | ||||
Sequence: F → V | ||||||
Sequence conflict | 478 | in Ref. 8; CAA57541 | ||||
Sequence: A → T | ||||||
Sequence conflict | 544 | in Ref. 8; CAA57541 | ||||
Sequence: Q → H |
Polymorphism
Genetic variation in SLC11A1 is associated with susceptibility to infection with Mycobacterium ulcerans [MIM:610446].
Genetic variations in SLC11A1 determine Mycobacterium tuberculosis susceptibility [MIM:607948].
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L32185 EMBL· GenBank· DDBJ | AAA57521.1 EMBL· GenBank· DDBJ | mRNA | ||
L38593 EMBL· GenBank· DDBJ | AAA57557.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
L38592 EMBL· GenBank· DDBJ | AAA57557.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF229163 EMBL· GenBank· DDBJ | AAG15405.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AC021016 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH471063 EMBL· GenBank· DDBJ | EAW70611.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC071165 EMBL· GenBank· DDBJ | AAH71165.1 EMBL· GenBank· DDBJ | mRNA | ||
D50402 EMBL· GenBank· DDBJ | BAA08907.1 EMBL· GenBank· DDBJ | mRNA | ||
D50403 EMBL· GenBank· DDBJ | BAA08908.1 EMBL· GenBank· DDBJ | mRNA | ||
D38171 EMBL· GenBank· DDBJ | BAA07370.1 EMBL· GenBank· DDBJ | mRNA | ||
X82015 EMBL· GenBank· DDBJ | CAA57541.1 EMBL· GenBank· DDBJ | Genomic DNA |