P49050 · NIA_PICAN

Function

function

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
FAD (UniProtKB | Rhea| CHEBI:57692 )

Note: Binds 1 FAD.
heme (UniProtKB | Rhea| CHEBI:30413 )

Note: Binds 1 heme group. The heme group is called cytochrome b-557.
Mo-molybdopterin (UniProtKB | Rhea| CHEBI:71302 )

Note: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site137Mo (UniProtKB | ChEBI) of Mo-molybdopterin (UniProtKB | ChEBI)
Binding site538Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue
Binding site561Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue
Binding site655-658FAD (UniProtKB | ChEBI)
Binding site672-676FAD (UniProtKB | ChEBI)
Binding site677FAD (UniProtKB | ChEBI)
Binding site687-689FAD (UniProtKB | ChEBI)
Binding site737FAD (UniProtKB | ChEBI)
Binding site740FAD (UniProtKB | ChEBI)
Binding site829-838NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionFAD binding
Molecular Functionheme binding
Molecular Functionmolybdenum ion binding
Molecular Functionmolybdopterin cofactor binding
Molecular Functionnitrate reductase (NADPH) activity
Molecular Functionsulfite oxidase activity
Biological Processnitrate assimilation
Biological Processnitric oxide biosynthetic process
Biological Processsulfur compound metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nitrate reductase [NADPH]
  • EC number
  • Short names
    NR

Gene names

    • Name
      YNR1

Organism names

  • Taxonomic identifier
  • Strain
    • ATCC 14754 / CBS 1976 / JCM 3620 / NBRC 0799 / NCYC 495 / NRRL Y-1798 / VKM Y-1397
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Pichiaceae > Ogataea

Accessions

  • Primary accession
    P49050

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00001660441-859Nitrate reductase [NADPH]
Disulfide bond383Interchain

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain502-578Cytochrome b5 heme-binding
Domain602-713FAD-binding FR-type

Sequence similarities

Belongs to the nitrate reductase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    859
  • Mass (Da)
    98,534
  • Last updated
    1996-02-01 v1
  • Checksum
    79569977B01E3967
MDSIVTEVTYGLEIKKIKDITELPFPVRQDSPLTEVLPTDLKTKDNFVARDPDLLRLTGSHPFNSEPPLTKLYDSGFLTPVSLHFVRNHGPVPYVPDENILDWEVSIEGMVETPYKIKLSDIMEQFDIYSTPVTMVCAGNRRKEQNMVKKGAGFNWGAAGTSTSLWTGCMLGDVIGKARPSKRARFVWMEGADNPANGAYRTCIRLSWCMDPERCIMIAYQQNGEWLHPDHGKPLRVVIPGVIGGRSVKWLKKLVVSDRPSENWYHYFDNRVLPTMVTPEMAKSDDRWWKDERYAIYDLNLQTIICKPENQQVIKISEDEYEIAGFGYNGGGVRIGRIEVSLDKGKSWKLADIDYPEDRYREAGYFRLFGGLVNVCDRMSCLCWCFWKLKVPLSELARSKDILIRGMDERMMVQPRTMYWNVTSMLNNWWYRVAIIREGESLRFEHPVVANKPGGWMDRVKAEGGDILDNNWGEVDDTVKQAERRPHIDEDLEMMCNREKMDVVIKYSEFEAHKDSETEPWFAVKGQVFDGSSYLEDHPGGAQSILMVSGEDATDDFIAIHSSFAKKLLPSMHLGRLEEVSSVTKVKSVEQNVKREVLLDPRKWHKITLAEKEVISSDSRIFKFDLEHSEQLSGLPTGKHLFLRLKDSSGKYVMRAYTPKSSNSLRGRLEILIKVYFPNREYPNGGIMTNLIENLQVGNQIEVKGPVGEFEYVKCGHCSFNNKPYQMKHFVMISGGSGITPTYQVLQAIFSDPEDRTSVQLFFGNKKVDDILLREELDHIQEKYPEQFKVDYSLSDLDHLPENWSGVRGRLTFDILDTYVRGKKMGEYMLLVCGPPGMNGVVENWCNARKLDKQYVVYF

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z49110
EMBL· GenBank· DDBJ
CAA88925.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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