P48841 · GANA_CELJU
- ProteinArabinogalactan endo-beta-1,4-galactanase
- GeneganB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids376 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
Catalytic activity
Cofactor
Note: Binds 1 Ca2+ ion per subunit.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
2.3 mM | 2,4-dinitrophenyl-beta-galactobioside | 7 | 37 |
pH Dependence
Optimum pH is 7.5.
Temperature Dependence
Optimum temperature is 60 degrees Celsius.
Features
Showing features for active site, binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | arabinogalactan endo-1,4-beta-galactosidase activity | |
Molecular Function | glucosidase activity | |
Molecular Function | metal ion binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameArabinogalactan endo-beta-1,4-galactanase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Cellvibrionales > Cellvibrionaceae > Cellvibrio
Accessions
- Primary accessionP48841
- Secondary accessions
Proteomes
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 161 | Partial loss of activity. | ||||
Sequence: E → A | ||||||
Mutagenesis | 270 | Complete loss of activity. | ||||
Sequence: E → A |
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-17 | |||||
Sequence: MKKKILAATAILLAAIA | ||||||
Chain | PRO_0000012220 | 18-376 | Arabinogalactan endo-beta-1,4-galactanase | |||
Sequence: NTGVADNTPFYVGADLSYVNEMESCGATYRDQGKKVDPFQLFADKGADLVRVRLWHNATWTKYSDLKDVSKTLKRAKNAGMKTLLDFHYSDTWTDPEKQFIPKAWAHITDTKELAKALYDYTTDTLASLDQQQLLPNLVQVGNETNIEILQAEDTLVHGIPNWQRNATLLNSGVNAVRDYSKKTGKPIQVVLHIAQPENALWWFKQAKENGVIDYDVIGLSYYPQWSEYSLPQLPDAIAELQNTYHKPVMIVETAYPWTLHNFDQAGNVLGEKAVQPEFPASPRGQLTYLLTLTQLVKSAGGMGVIYWEPAWVSTRCRTLWGKGSHWENASFFDATRKNNALPAFLFFKADYQASAQAE |
Expression
Induction
By galactan.
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length376
- Mass (Da)42,315
- Last updated1996-02-01 v1
- ChecksumBEC4F7243C6CAE10
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X91885 EMBL· GenBank· DDBJ | CAA62990.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP000934 EMBL· GenBank· DDBJ | ACE83887.1 EMBL· GenBank· DDBJ | Genomic DNA |