P48841 · GANA_CELJU

Function

Catalytic activity

  • The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.
    EC:3.2.1.89 (UniProtKB | ENZYME | Rhea)

Cofactor

Ca2+ (UniProtKB | Rhea| CHEBI:29108 )

Note: Binds 1 Ca2+ ion per subunit.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
2.3 mM2,4-dinitrophenyl-beta-galactobioside737

pH Dependence

Optimum pH is 7.5.

Temperature Dependence

Optimum temperature is 60 degrees Celsius.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site161Proton donor
Active site270Nucleophile
Binding site281Ca2+ (UniProtKB | ChEBI)
Binding site285Ca2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionarabinogalactan endo-1,4-beta-galactosidase activity
Molecular Functionglucosidase activity
Molecular Functionmetal ion binding

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Arabinogalactan endo-beta-1,4-galactanase
  • EC number
  • Alternative names
    • Endo-1,4-beta-galactanase (Galactanase)

Gene names

    • Name
      ganB
    • Synonyms
      gal53A-2, galA, ganA
    • Ordered locus names
      CJA_0497

Organism names

Accessions

  • Primary accession
    P48841
  • Secondary accessions
    • B3PIY6

Proteomes

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis161Partial loss of activity.
Mutagenesis270Complete loss of activity.

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-17
ChainPRO_000001222018-376Arabinogalactan endo-beta-1,4-galactanase

Expression

Induction

By galactan.

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the glycosyl hydrolase 53 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    376
  • Mass (Da)
    42,315
  • Last updated
    1996-02-01 v1
  • Checksum
    BEC4F7243C6CAE10
MKKKILAATAILLAAIANTGVADNTPFYVGADLSYVNEMESCGATYRDQGKKVDPFQLFADKGADLVRVRLWHNATWTKYSDLKDVSKTLKRAKNAGMKTLLDFHYSDTWTDPEKQFIPKAWAHITDTKELAKALYDYTTDTLASLDQQQLLPNLVQVGNETNIEILQAEDTLVHGIPNWQRNATLLNSGVNAVRDYSKKTGKPIQVVLHIAQPENALWWFKQAKENGVIDYDVIGLSYYPQWSEYSLPQLPDAIAELQNTYHKPVMIVETAYPWTLHNFDQAGNVLGEKAVQPEFPASPRGQLTYLLTLTQLVKSAGGMGVIYWEPAWVSTRCRTLWGKGSHWENASFFDATRKNNALPAFLFFKADYQASAQAE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X91885
EMBL· GenBank· DDBJ
CAA62990.1
EMBL· GenBank· DDBJ
Genomic DNA
CP000934
EMBL· GenBank· DDBJ
ACE83887.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp