P48813 · GNP1_YEAST
- ProteinHigh-affinity glutamine permease
- GeneGNP1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids663 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
High affinity transport of glutamine. Also transports Leu, Ser, Thr, Cys, Met and Asn.
Miscellaneous
Present with 10600 molecules/cell in log phase SD medium.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cell periphery | |
Cellular Component | cellular bud neck | |
Cellular Component | endoplasmic reticulum | |
Cellular Component | membrane | |
Cellular Component | mitochondrial membrane | |
Cellular Component | mitochondrion | |
Molecular Function | amino acid transmembrane transporter activity | |
Molecular Function | L-proline transmembrane transporter activity | |
Biological Process | amino acid transmembrane transport | |
Biological Process | amino acid transport | |
Biological Process | intracellular sphingolipid homeostasis | |
Biological Process | serine import across plasma membrane | |
Biological Process | transmembrane transport |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameHigh-affinity glutamine permease
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP48813
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Mitochondrion membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-154 | Cytoplasmic | ||||
Sequence: MTLGNRRHGRNNEGSSNMNMNRNDLDDVSHYEMKEIQPKEKQIGSIEPENEVEYFEKTVEKTIENMEYEGEHHASYLRRFIDSFRRAEGSHANSPDSSNSNGTTPISTKDSSSQLDNELNRKSSYITVDGIKQSPQEQEQKQENLKKSIKPRHT | ||||||
Transmembrane | 155-175 | Helical | ||||
Sequence: VMMSLGTGIGTGLLVGNSKVL | ||||||
Topological domain | 176-177 | Mitochondrial intermembrane | ||||
Sequence: NN | ||||||
Transmembrane | 178-198 | Helical | ||||
Sequence: AGPGGLIIGYAIMGSCVYCII | ||||||
Topological domain | 199-219 | Cytoplasmic | ||||
Sequence: QACGELAVIYSDLIGGFNTYP | ||||||
Transmembrane | 220-240 | Helical | ||||
Sequence: LFLVDPALGFSVAWLFCLQWL | ||||||
Topological domain | 241-264 | Mitochondrial intermembrane | ||||
Sequence: CVCPLELVTASMTIKYWTTSVNPD | ||||||
Transmembrane | 265-285 | Helical | ||||
Sequence: VFVVIFYVLIVVINVFGAKGY | ||||||
Topological domain | 286-289 | Cytoplasmic | ||||
Sequence: AEAD | ||||||
Transmembrane | 290-310 | Helical | ||||
Sequence: FFFNCCKILMIVGFFILAIII | ||||||
Topological domain | 311-342 | Mitochondrial intermembrane | ||||
Sequence: DCGGAGTDGYIGSKYWRDPGAFRGDTPIQRFK | ||||||
Transmembrane | 343-363 | Helical | ||||
Sequence: GVVATFVTAAFAFGMSEQLAM | ||||||
Topological domain | 364-381 | Cytoplasmic | ||||
Sequence: TASEQSNPRKAIPSAAKK | ||||||
Transmembrane | 382-402 | Helical | ||||
Sequence: MIYRILFVFLASLTLVGFLVP | ||||||
Topological domain | 403-432 | Mitochondrial intermembrane | ||||
Sequence: YTSDQLLGAAGSATKASPYVIAVSSHGVRV | ||||||
Transmembrane | 433-453 | Helical | ||||
Sequence: VPHFINAVILLSVLSVANGAF | ||||||
Topological domain | 454-481 | Cytoplasmic | ||||
Sequence: YTSSRILMSLAKQGNAPKCFDYIDREGR | ||||||
Transmembrane | 482-502 | Helical | ||||
Sequence: PAAAMLVSALFGVIAFCASSK | ||||||
Topological domain | 503-506 | Mitochondrial intermembrane | ||||
Sequence: KEED | ||||||
Transmembrane | 507-527 | Helical | ||||
Sequence: VFTWLLAISGLSQLFTWITIC | ||||||
Topological domain | 528-560 | Cytoplasmic | ||||
Sequence: LSHIRFRRAMKVQGRSLGEVGYKSQVGVWGSAY | ||||||
Transmembrane | 561-581 | Helical | ||||
Sequence: AVLMMVLALIAQFWVAIAPIG | ||||||
Topological domain | 582-590 | Mitochondrial intermembrane | ||||
Sequence: GGGKLSAQS | ||||||
Transmembrane | 591-611 | Helical | ||||
Sequence: FFENYLAMPIWIALYIFYKVW | ||||||
Topological domain | 612-663 | Cytoplasmic | ||||
Sequence: KKDWSLFIPADKVDLVSHRNIFDEELLKQEDEEYKERLRNGPYWKRVLDFWC |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000054152 | 1-663 | High-affinity glutamine permease | |||
Sequence: MTLGNRRHGRNNEGSSNMNMNRNDLDDVSHYEMKEIQPKEKQIGSIEPENEVEYFEKTVEKTIENMEYEGEHHASYLRRFIDSFRRAEGSHANSPDSSNSNGTTPISTKDSSSQLDNELNRKSSYITVDGIKQSPQEQEQKQENLKKSIKPRHTVMMSLGTGIGTGLLVGNSKVLNNAGPGGLIIGYAIMGSCVYCIIQACGELAVIYSDLIGGFNTYPLFLVDPALGFSVAWLFCLQWLCVCPLELVTASMTIKYWTTSVNPDVFVVIFYVLIVVINVFGAKGYAEADFFFNCCKILMIVGFFILAIIIDCGGAGTDGYIGSKYWRDPGAFRGDTPIQRFKGVVATFVTAAFAFGMSEQLAMTASEQSNPRKAIPSAAKKMIYRILFVFLASLTLVGFLVPYTSDQLLGAAGSATKASPYVIAVSSHGVRVVPHFINAVILLSVLSVANGAFYTSSRILMSLAKQGNAPKCFDYIDREGRPAAAMLVSALFGVIAFCASSKKEEDVFTWLLAISGLSQLFTWITICLSHIRFRRAMKVQGRSLGEVGYKSQVGVWGSAYAVLMMVLALIAQFWVAIAPIGGGGKLSAQSFFENYLAMPIWIALYIFYKVWKKDWSLFIPADKVDLVSHRNIFDEELLKQEDEEYKERLRNGPYWKRVLDFWC | ||||||
Modified residue | 29 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 34 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K | ||||||
Cross-link | 39 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K | ||||||
Cross-link | 41 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K | ||||||
Cross-link | 61 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K | ||||||
Modified residue | 113 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 124 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 127 | Phosphothreonine | ||||
Sequence: T | ||||||
Cross-link | 132 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P48813 | SHR3 Q02774 | 3 | EBI-7758, EBI-17099 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-24 | Disordered | ||||
Sequence: MTLGNRRHGRNNEGSSNMNMNRND | ||||||
Region | 87-117 | Disordered | ||||
Sequence: AEGSHANSPDSSNSNGTTPISTKDSSSQLDN | ||||||
Compositional bias | 90-117 | Polar residues | ||||
Sequence: SHANSPDSSNSNGTTPISTKDSSSQLDN |
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length663
- Mass (Da)73,598
- Last updated1996-10-01 v2
- Checksum9A5ED9CF7C1DD977
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 90-117 | Polar residues | ||||
Sequence: SHANSPDSSNSNGTTPISTKDSSSQLDN | ||||||
Sequence conflict | 319 | in Ref. 1; AAB48002 | ||||
Sequence: G → S |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U21643 EMBL· GenBank· DDBJ | AAB48002.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U33057 EMBL· GenBank· DDBJ | AAB64950.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006938 EMBL· GenBank· DDBJ | DAA12340.1 EMBL· GenBank· DDBJ | Genomic DNA |