P48636 · LOGH_PSEAE
- ProteinCytokinin riboside 5'-monophosphate phosphoribohydrolase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids195 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the hydrolytic removal of ribose 5'-monophosphate from nitrogen N6-modified adenosines, the final step of bioactive cytokinin synthesis (Probable). Exhibits phosphoribohydrolase activity against AMP in vitro (PubMed:29901273).
Catalytic activity
- H2O + N6-(dimethylallyl)adenosine 5'-phosphate = D-ribose 5-phosphate + N6-dimethylallyladenine
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 74 | substrate | ||||
Sequence: E | ||||||
Binding site | 92-93 | substrate | ||||
Sequence: RK | ||||||
Binding site | 109-115 | substrate | ||||
Sequence: GLGTLEE | ||||||
Binding site | 121 | substrate | ||||
Sequence: T |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | AMP nucleosidase activity | |
Molecular Function | cytokinin riboside 5'-monophosphate phosphoribohydrolase activity | |
Molecular Function | hydrolase activity, hydrolyzing N-glycosyl compounds | |
Biological Process | cytokinin biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytokinin riboside 5'-monophosphate phosphoribohydrolase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionP48636
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 89 | Almost complete loss of activity. | ||||
Sequence: M → A | ||||||
Mutagenesis | 92 | Almost complete loss of activity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 112 | Almost complete loss of activity. | ||||
Sequence: T → A | ||||||
Mutagenesis | 114 | Almost complete loss of activity. | ||||
Sequence: E → A | ||||||
Mutagenesis | 115 | Almost complete loss of activity. | ||||
Sequence: E → A | ||||||
Mutagenesis | 118 | Retains 8% of wild-type activity. | ||||
Sequence: E → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000206257 | 1-195 | Cytokinin riboside 5'-monophosphate phosphoribohydrolase | |||
Sequence: MTLRSVCVFCGASPGASPVYQEAAVALGRHLAERGLTLVYGGGAVGLMGTVADAALAAGGEVIGIIPQSLQEAEIGHKGLTRLEVVDGMHARKARMAELADAFIALPGGLGTLEELFEVWTWGQLGYHAKPLGLLEVNGFYDPLLTFLDHLVDERFVRAEHRGMLQRGASPEALLDALAAWTPSVAPKWVDRTPQ |
Proteomic databases
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length195
- Mass (Da)20,725
- Last updated2001-01-11 v2
- ChecksumA5784C919D80361C
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 60 | in Ref. 2; AAA25729 | ||||
Sequence: G → S |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE004091 EMBL· GenBank· DDBJ | AAG08308.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M30389 EMBL· GenBank· DDBJ | AAA25729.2 EMBL· GenBank· DDBJ | Genomic DNA |