P48445 · BPL1_YEAST
- ProteinBiotin--protein ligase
- GeneBPL1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids690 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Post-translational modification of specific protein by attachment of biotin. Acts on various carboxylases such as acetyl-CoA-carboxylase, pyruvate carboxylase, propionyl CoA carboxylase, and 3-methylcrotonyl CoA carboxylase.
Miscellaneous
Present with 1970 molecules/cell in log phase SD medium.
Catalytic activity
- apo-[methylmalonyl-CoA:pyruvate carboxytransferase] + ATP + biotin = AMP + diphosphate + H+ + holo-[methylmalonyl-CoA:pyruvate carboxytransferase]
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | biotin-[acetyl-CoA-carboxylase] ligase activity | |
Molecular Function | biotin-[methylcrotonoyl-CoA-carboxylase] ligase activity | |
Molecular Function | biotin-[methylmalonyl-CoA-carboxytransferase] ligase activity | |
Molecular Function | biotin-[propionyl-CoA-carboxylase (ATP-hydrolyzing)] ligase activity | |
Molecular Function | biotin-[pyruvate-carboxylase] ligase activity | |
Biological Process | protein modification process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBiotin--protein ligase
- EC number
- Alternative names
Including 4 domains:
- Recommended nameBiotin--[methylmalonyl-CoA-carboxytransferase] ligase
- EC number
- Recommended nameBiotin--[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase
- EC number
- Alternative names
- Recommended nameBiotin--[methylcrotonoyl-CoA-carboxylase] ligase
- EC number
- Recommended nameBiotin--[acetyl-CoA-carboxylase] ligase
- EC number
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP48445
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000064981 | 1-690 | Biotin--protein ligase | |||
Sequence: MNVLVYNGPGTTPGSVKHAVESLRDFLEPYYAVSTVNVKVLQTEPWMSKTSAVVFPGGADLPYVQACQPIISRLKHFVSKQGGVFIGFCAGGYFGTSRVEFAQGDPTMEVSGSRDLRFFPGTSRGPAYNGFQYNSEAGARAVKLNLPDGSQFSTYFNGGAVFVDADKFDNVEILATYAEHPDVPSSDSGKGQSENPAAVVLCTVGRGKVLLTGPHPEFNVRFMRKSTDKHFLETVVENLKAQEIMRLKFMRTVLTKTGLNCNNDFNYVRAPNLTPLFMASAPNKRNYLQEMENNLAHHGMHANNVELCSELNAETDSFQFYRGYRASYDAASSSLLHKEPDEVPKTVIFPGVDEDIPPFQYTPNFDMKEYFKYLNVQNTIGSLLLYGEVVTSTSTILNNNKSLLSSIPESTLLHVGTIQVSGRGRGGNTWINPKGVCASTAVVTMPLQSPVTNRNISVVFVQYLSMLAYCKAILSYAPGFSDIPVRIKWPNDLYALSPTYYKRKNLKLVNTGFEHTKLPLGDIEPAYLKISGLLVNTHFINNKYCLLLGCGINLTSDGPTTSLQTWIDILNEERQQLHLDLLPAIKAEKLQALYMNNLEVILKQFINYGAAEILPSYYELWLHSNQIVTLPDHGNTQAMITGITEDYGLLIAKELVSGSSTQFTGNVYNLQPDGNTFDIFKSLIAKKVQS |
Proteomic databases
PTM databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 378-606 | BPL/LPL catalytic | ||||
Sequence: NTIGSLLLYGEVVTSTSTILNNNKSLLSSIPESTLLHVGTIQVSGRGRGGNTWINPKGVCASTAVVTMPLQSPVTNRNISVVFVQYLSMLAYCKAILSYAPGFSDIPVRIKWPNDLYALSPTYYKRKNLKLVNTGFEHTKLPLGDIEPAYLKISGLLVNTHFINNKYCLLLGCGINLTSDGPTTSLQTWIDILNEERQQLHLDLLPAIKAEKLQALYMNNLEVILKQFI |
Sequence similarities
Belongs to the biotin--protein ligase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length690
- Mass (Da)76,363
- Last updated1996-02-01 v1
- ChecksumADA6BFE411C656AB
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U27182 EMBL· GenBank· DDBJ | AAC49057.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X96876 EMBL· GenBank· DDBJ | CAA65617.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z74189 EMBL· GenBank· DDBJ | CAA98714.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006938 EMBL· GenBank· DDBJ | DAA11717.1 EMBL· GenBank· DDBJ | Genomic DNA |