P48421 · C83A1_ARATH

Function

function

Involved in the metabolism of aliphatic and aromatic oximes (PubMed:12970475).
Involved in the biosynthesis of both short-chain and long-chain aliphatic glucosinolates (PubMed:12509530).

Catalytic activity

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
150 μMindole-3-acetaldoxime
156 μMp-hydroxyphenylacetaldoxime
556 μMphenylacetaldoxime
kcat is 140 min-1 with indole-3-acetaldoxime as substrate. kcat is 26 min-1 with p-hydroxyphenylacetaldoxime as substrate. kcat is 25 min-1 with phenylacetaldoxime as substrate.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site442Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentendoplasmic reticulum membrane
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
Biological Processglucosinolate biosynthetic process
Biological Processresponse to insect

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytochrome P450 83A1
  • EC number
  • Alternative names
    • CYPLXXXIII
    • Protein REDUCED EPIDERMAL FLUORESCENCE 2

Gene names

    • Name
      CYP83A1
    • Synonyms
      CYP83, REF2
    • ORF names
      F18A5.160
    • Ordered locus names
      At4g13770

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Columbia
    • cv. C24
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    P48421
  • Secondary accessions
    • O24429

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1-21Helical

Keywords

Phenotypes & Variants

Disruption phenotype

Reduced levels of all aliphatic glucosinolates and increased levels of indole-derived glucosinolates in leaves.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 15 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000521661-502Cytochrome P450 83A1

Proteomic databases

Expression

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    502
  • Mass (Da)
    57,449
  • Last updated
    1998-12-15 v2
  • Checksum
    7A0F6F36FD5D7577
MEDIIIGVVALAAVLLFFLYQKPKTKRYKLPPGPSPLPVIGNLLQLQKLNPQRFFAGWAKKYGPILSYRIGSRTMVVISSAELAKELLKTQDVNFADRPPHRGHEFISYGRRDMALNHYTPYYREIRKMGMNHLFSPTRVATFKHVREEEARRMMDKINKAADKSEVVDISELMLTFTNSVVCRQAFGKKYNEDGEEMKRFIKILYGTQSVLGKIFFSDFFPYCGFLDDLSGLTAYMKECFERQDTYIQEVVNETLDPKRVKPETESMIDLLMGIYKEQPFASEFTVDNVKAVILDIVVAGTDTAAAAVVWGMTYLMKYPQVLKKAQAEVREYMKEKGSTFVTEDDVKNLPYFRALVKETLRIEPVIPLLIPRACIQDTKIAGYDIPAGTTVNVNAWAVSRDEKEWGPNPDEFRPERFLEKEVDFKGTDYEFIPFGSGRRMCPGMRLGAAMLEVPYANLLLSFNFKLPNGMKPDDINMDVMTGLAMHKSQHLKLVPEKVNKY

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict277in Ref. 1; AAA79982

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U18929
EMBL· GenBank· DDBJ
AAA79982.1
EMBL· GenBank· DDBJ
mRNA
D78599
EMBL· GenBank· DDBJ
BAA28532.1
EMBL· GenBank· DDBJ
mRNA
U69134
EMBL· GenBank· DDBJ
AAB71623.1
EMBL· GenBank· DDBJ
Genomic DNA
AL035528
EMBL· GenBank· DDBJ
CAB36841.1
EMBL· GenBank· DDBJ
Genomic DNA
AL161537
EMBL· GenBank· DDBJ
CAB78419.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002687
EMBL· GenBank· DDBJ
AEE83323.1
EMBL· GenBank· DDBJ
Genomic DNA
AF428469
EMBL· GenBank· DDBJ
AAL16238.1
EMBL· GenBank· DDBJ
mRNA
AY075697
EMBL· GenBank· DDBJ
AAL77703.1
EMBL· GenBank· DDBJ
mRNA
AY102146
EMBL· GenBank· DDBJ
AAM26713.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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