P48421 · C83A1_ARATH
- ProteinCytochrome P450 83A1
- GeneCYP83A1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids502 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the metabolism of aliphatic and aromatic oximes (PubMed:12970475).
Involved in the biosynthesis of both short-chain and long-chain aliphatic glucosinolates (PubMed:12509530).
Involved in the biosynthesis of both short-chain and long-chain aliphatic glucosinolates (PubMed:12509530).
Catalytic activity
- an (E)-omega-(methylsulfanyl)-alkanal oxime + glutathione + O2 + reduced [NADPH--hemoprotein reductase] = an S-[(1E)-1-(hydroxyimino)-omega-(methylsulfanyl)alkyl]-L-glutathione + H+ + 2 H2O + oxidized [NADPH--hemoprotein reductase]
an (E)-ω-(methylsulfanyl)-alkanal oxime RHEA-COMP:13114 + CHEBI:57925 + CHEBI:15379 + RHEA-COMP:11964 = an S-[(1E)-1-(hydroxyimino)-ω-(methylsulfanyl)alkyl]-L-glutathione RHEA-COMP:13138 + CHEBI:15378 + 2 CHEBI:15377 + RHEA-COMP:11965
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
150 μM | indole-3-acetaldoxime | |||||
156 μM | p-hydroxyphenylacetaldoxime | |||||
556 μM | phenylacetaldoxime |
kcat is 140 min-1 with indole-3-acetaldoxime as substrate. kcat is 26 min-1 with p-hydroxyphenylacetaldoxime as substrate. kcat is 25 min-1 with phenylacetaldoxime as substrate.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen | |
Biological Process | glucosinolate biosynthetic process | |
Biological Process | response to insect |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytochrome P450 83A1
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionP48421
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 1-21 | Helical | ||||
Sequence: MEDIIIGVVALAAVLLFFLYQ |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Reduced levels of all aliphatic glucosinolates and increased levels of indole-derived glucosinolates in leaves.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 15 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000052166 | 1-502 | Cytochrome P450 83A1 | |||
Sequence: MEDIIIGVVALAAVLLFFLYQKPKTKRYKLPPGPSPLPVIGNLLQLQKLNPQRFFAGWAKKYGPILSYRIGSRTMVVISSAELAKELLKTQDVNFADRPPHRGHEFISYGRRDMALNHYTPYYREIRKMGMNHLFSPTRVATFKHVREEEARRMMDKINKAADKSEVVDISELMLTFTNSVVCRQAFGKKYNEDGEEMKRFIKILYGTQSVLGKIFFSDFFPYCGFLDDLSGLTAYMKECFERQDTYIQEVVNETLDPKRVKPETESMIDLLMGIYKEQPFASEFTVDNVKAVILDIVVAGTDTAAAAVVWGMTYLMKYPQVLKKAQAEVREYMKEKGSTFVTEDDVKNLPYFRALVKETLRIEPVIPLLIPRACIQDTKIAGYDIPAGTTVNVNAWAVSRDEKEWGPNPDEFRPERFLEKEVDFKGTDYEFIPFGSGRRMCPGMRLGAAMLEVPYANLLLSFNFKLPNGMKPDDINMDVMTGLAMHKSQHLKLVPEKVNKY |
Proteomic databases
Expression
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Length502
- Mass (Da)57,449
- Last updated1998-12-15 v2
- Checksum7A0F6F36FD5D7577
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 277 | in Ref. 1; AAA79982 | ||||
Sequence: K → T |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U18929 EMBL· GenBank· DDBJ | AAA79982.1 EMBL· GenBank· DDBJ | mRNA | ||
D78599 EMBL· GenBank· DDBJ | BAA28532.1 EMBL· GenBank· DDBJ | mRNA | ||
U69134 EMBL· GenBank· DDBJ | AAB71623.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL035528 EMBL· GenBank· DDBJ | CAB36841.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL161537 EMBL· GenBank· DDBJ | CAB78419.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002687 EMBL· GenBank· DDBJ | AEE83323.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF428469 EMBL· GenBank· DDBJ | AAL16238.1 EMBL· GenBank· DDBJ | mRNA | ||
AY075697 EMBL· GenBank· DDBJ | AAL77703.1 EMBL· GenBank· DDBJ | mRNA | ||
AY102146 EMBL· GenBank· DDBJ | AAM26713.1 EMBL· GenBank· DDBJ | mRNA |