P48419 · C75A3_PETHY
- ProteinFlavonoid 3',5'-hydroxylase 2
- GeneCYP75A3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids508 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Catalyzes the 3'5'-hydroxylation of naringenin and eriodictyol to form 5,7,3,'4',5'-pentahydroxyflavanone and 3',5'-hydroxylation of dihydrokaempferol and dihydroquercetin to form dihydromyricetin.
Catalytic activity
- a 3',5'-unsubstituted flavanone + 2 reduced [NADPH--hemoprotein reductase] + 2 O2 = a 3',5'-dihydroxyflavanone + 2 oxidized [NADPH--hemoprotein reductase] + 2 H2O + 2 H+
Cofactor
Pathway
Pigment biosynthesis; anthocyanin biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 443 | Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Molecular Function | flavonoid 3',5'-hydroxylase activity | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Biological Process | anthocyanin-containing compound biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFlavonoid 3',5'-hydroxylase 2
- EC number
- Short namesF3'5'H
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Petunioideae > Petunia
Accessions
- Primary accessionP48419
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000052130 | 1-508 | Flavonoid 3',5'-hydroxylase 2 | ||
Expression
Tissue specificity
Flowers.
Structure
Sequence
- Sequence statusComplete
- Length508
- Mass (Da)56,721
- Last updated1996-02-01 v1
- Checksum70989FF2B3AD4FD8
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z22544 EMBL· GenBank· DDBJ | CAA80265.1 EMBL· GenBank· DDBJ | mRNA |