P48269 · CCSA_CHLRE
- ProteinCytochrome c biogenesis protein CcsA
- GeneccsA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids353 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Required during biogenesis of c-type cytochromes (cytochrome c6 and cytochrome f) at the step of heme attachment.
Miscellaneous
Met-1 is the initiator; however when Met-1 is mutated a low amount of protein may be produced, probably from Met-20.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chloroplast thylakoid membrane | |
Molecular Function | heme binding | |
Biological Process | cytochrome complex assembly |
Keywords
- Biological process
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameCytochrome c biogenesis protein CcsA
Gene names
Encoded on
- Chloroplast
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Chlorophyta > core chlorophytes > Chlorophyceae > CS clade > Chlamydomonadales > Chlamydomonadaceae > Chlamydomonas
Accessions
- Primary accessionP48269
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Plastid, chloroplast thylakoid membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane, topological domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 15-32 | Helical | ||||
Sequence: NATFCMLFLTTFLYWFYT | ||||||
Topological domain | 33-208 | Lumenal | ||||
Sequence: AFYSTNPQQIINPLSLTNIKTNFTYPDGFQANNINVSTTYLPINPVLNTEREEQPEANGTNGLLGVSSLVVNLKSIAIPRIMMGVSNLLLVLLLLVRWEKSGHFPLSNLYESLMFLAWCCTFLYLLYCTSFTLLVEKMLGSLIAPCSLLMNAFATFSLPKEMQQASPLVPALQSNW | ||||||
Transmembrane | 209-233 | Helical | ||||
Sequence: LMMHVTVMIISYATLIIGSLLSILF | ||||||
Topological domain | 234-260 | Stromal | ||||
Sequence: LILFKHKKGTPKKYDNFINNLDALSYR | ||||||
Transmembrane | 261-278 | Helical | ||||
Sequence: IIGLGFPFLTIGILSGAV | ||||||
Topological domain | 279-295 | Lumenal | ||||
Sequence: WANEAWGSYWSWDPKET | ||||||
Transmembrane | 296-313 | Helical | ||||
Sequence: WALLTWLVFAIYLHTRLT | ||||||
Topological domain | 314-323 | Stromal | ||||
Sequence: KGWEGEKPAI | ||||||
Transmembrane | 324-342 | Helical | ||||
Sequence: IAAVGFLVVWFCYLGVNLI | ||||||
Topological domain | 343-353 | Lumenal | ||||
Sequence: GEGLHSYGFFN |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 1 | Almost no c-type holocytochrome formation. | ||||
Sequence: M → D | ||||||
Mutagenesis | 20 | No effect on c-type holocytochrome formation. | ||||
Sequence: M → A | ||||||
Mutagenesis | 212 | Abrogates production of c-type holocytochrome. | ||||
Sequence: H → D | ||||||
Mutagenesis | 279 | Abrogates production of c-type holocytochrome. | ||||
Sequence: W → A | ||||||
Mutagenesis | 284 | Severely reduces amounts of c-type holocytochrome produced. | ||||
Sequence: W → A | ||||||
Mutagenesis | 288 | Reduced amounts of c-type holocytochromes produced. | ||||
Sequence: W → A | ||||||
Mutagenesis | 288-290 | Reduced amounts of c-type holocytochromes produced; a more severe reduction than seen with either single mutation. | ||||
Sequence: WSW → ASA | ||||||
Mutagenesis | 290 | Reduced amounts of c-type holocytochromes produced. | ||||
Sequence: W → A | ||||||
Mutagenesis | 291 | Abrogates production of c-type holocytochrome. No assembly of cytochrome c6 occurs. | ||||
Sequence: D → A | ||||||
Mutagenesis | 291 | Abrogates production of c-type holocytochrome. Permits small amounts of cytochrome c6 assembly. | ||||
Sequence: D → E or N | ||||||
Mutagenesis | 296 | No effect on activity. | ||||
Sequence: W → A | ||||||
Mutagenesis | 301 | No effect on activity. | ||||
Sequence: W → A | ||||||
Mutagenesis | 309 | Abrogates production of c-type holocytochrome. | ||||
Sequence: H → E | ||||||
Mutagenesis | 347 | Abrogates production of c-type holocytochrome. | ||||
Sequence: H → A or E |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000201600 | 1-353 | Cytochrome c biogenesis protein CcsA | |||
Sequence: MNFVNLEQIENSLRNATFCMLFLTTFLYWFYTAFYSTNPQQIINPLSLTNIKTNFTYPDGFQANNINVSTTYLPINPVLNTEREEQPEANGTNGLLGVSSLVVNLKSIAIPRIMMGVSNLLLVLLLLVRWEKSGHFPLSNLYESLMFLAWCCTFLYLLYCTSFTLLVEKMLGSLIAPCSLLMNAFATFSLPKEMQQASPLVPALQSNWLMMHVTVMIISYATLIIGSLLSILFLILFKHKKGTPKKYDNFINNLDALSYRIIGLGFPFLTIGILSGAVWANEAWGSYWSWDPKETWALLTWLVFAIYLHTRLTKGWEGEKPAIIAAVGFLVVWFCYLGVNLIGEGLHSYGFFN |
Proteomic databases
Interaction
Structure
Family & Domains
Sequence similarities
Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length353
- Mass (Da)39,983
- Last updated2003-07-11 v2
- Checksum3D46ABDF51044C4B
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 342 | in Ref. 1; AAA76600 | ||||
Sequence: I → T |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U15556 EMBL· GenBank· DDBJ | AAA76600.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U09190 EMBL· GenBank· DDBJ | AAB03815.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
FJ423446 EMBL· GenBank· DDBJ | ACJ50117.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK000554 EMBL· GenBank· DDBJ | DAA00929.1 EMBL· GenBank· DDBJ | Genomic DNA |